A Review of Bovine Urothelial Tumours and Tumour-Like Lesions of the Urinary Bladder

Roperto, Sante; Borzacchiello, Giuseppe; Brun, R.; Leonardi, Leonardo; Maiolino, Paola; Martano, Manuela; Paciello, Orlando; Papparella, Serenella; Restucci, Brunella; Russo, Valeria; Salvatore, Giuliana; Urraro, Chiara; Roperto, F.

doi:10.1016/j.jcpa.2009.08.156

Cited by 69 publications

(89 citation statements)

References 76 publications

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“…Many of HPV E5 functions have been first elucidated in BPV models, while some BPV E5 activities are not even shared by HPV E5, such as the activation of PDGFR and the tyrosine kinase c-src in bovine bladder carcinomas. Bovine papillomavirus type 2 and, less frequently, BPV-1 cause a non-productive infection of the urothelium in cattle, which has been associated with the development of urinary bladder tumours [22][23]. A similar pathology showing identical features was recently described in Turkish water buffaloes [24].…”

Section: Bpv-induced Cell Transformationmentioning

confidence: 69%

Bovine papillomavirus: opening new trends for comparative pathology

Costa

Medeiros

2013

Arch Virol

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Section: Bpv-induced Cell Transformationmentioning

confidence: 69%

Bovine papillomavirus: opening new trends for comparative pathology

Costa

Medeiros

2013

Arch Virol

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“…In .90 % of cases, tumours of the urinary bladder are responsible for a severe clinical syndrome known as chronic enzootic haematuria (Maxie, 1993). The incidence of bladder tumours varies among cattle grazing on pastures contaminated with bracken fern but may be .90 % in adult animals (Pamukcu et al, 1976;Roperto et al, 2010a, b).…”

Section: Introductionmentioning

confidence: 99%

“…In this context, cattle may serve as an animal model and may continue to lead the way in studying the comparative biology of PVs. Much of our understanding of PVs, including their life cycle and other relevant aspects of pathogenesis, has benefited and may continue to do so from investigations on animal models Roperto et al, 2010a).Finally, the presence of structural L1 capsid proteins in PBMCs should be regarded as an marker of productive BPV-2 infection, thus opening novel scenarios in the epidemiology and pathophysiology of these challenging virus infections. …”

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confidence: 99%

“…Tissues fixed in 10 % neutral buffered formalin were routinely processed for paraffin embedding. Histological diagnosis was assessed on 5 mm haematoxylin and eosin-stained sections using the morphological criteria suggested in the recent report on the new histological classification of urothelial tumours of the urinary bladder of cattle (Roperto et al, 2010a).Cell isolation and sorting. Peripheral blood (50 ml) was collected in EDTA-containing tubes and submitted to centrifugation for 40 min at 1000 g. PMNs were isolated according to the method of Carlson & Kaneko (1973) after removal of the buffy coat and erythrocyte lysis.…”

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confidence: 99%

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PBMCs are additional sites of productive infection of bovine papillomavirus type 2

Roperto

Comazzi

Ciusani

et al. 2011

Journal of General Virology

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Bovine papillomavirus type 2 (BPV-2) is an oncogenic virus infecting both epithelial and mesenchymal cells. Its life cycle, similar to other papillomaviruses (PVs), appears to be linked to epithelial differentiation. Human and bovine PVs have been known to reside in a latent, episomal form in PBMCs; therefore, it is believed that blood cells, like all mesenchymal cells, function as non-permissive carriers. Here, for the first time in veterinary and comparative medicine, the BPV-2 E5 oncoprotein and the major structural L1 capsid protein, known to be expressed only in productive infections, were shown to occur in defined subsets of PBMCs. E5 oncoprotein was detected in sorted T-and B-cells as well as in monocytes by flow cytometry and Western blot analysis. However, CD4 + and CD8 + lymphocytes appeared to be the main circulating targets of the virus, thus possibly representing the most important reservoir of active BPV-2 in blood. L1 protein was identified by flow cytometry in a population of blood cells recognized as lymphocytes by morphological scatter properties. Western blot analysis was performed on lysates obtained from the sorted subpopulations of PBMCs and detected L1 protein in CD4 + and CD8 + cells only. Thus, this study showed that CD4 + and CD8 + lymphocytes are permissive for BPV-2 and are new, hitherto unknown sites of productive PV infection. In light of these observations, the life cycle of PVs needs to be revisited to gain novel insights into the epidemiology of BPV infection and the pathogenesis of related diseases. INTRODUCTIONBovine papillomaviruses (BPVs) are a heterogeneous group of viruses responsible for tumours of the skin, genital and paragenital area, eye, upper gastrointestinal tract and urinary bladder (IARC, 2007). BPVs, like all other papillomaviruses (PVs), are usually strictly species specific. However, cases of cross-species infection are known to occur. BPV type 1 (BPV-1) and BPV-2, belonging to the genus Deltapapillomavirus (de Villiers et al., 2004), are responsible for infections in equids resulting in sarcoids (Chambers et al., 2003;Trenfield et al., 1985), as well as in bison and water buffaloes leading to warts (Literák et al., 2006;Silvestre et al., 2009). In addition, a variant of BPV-8, classified in the genus Epsilonpapillomavirus, also causes papillomas of the skin in bison (Tomita et al., 2007). More recently, it has been suggested that BPVs could be responsible for cutaneous sarcoids in cats and captive African lions (Munday & Knight, 2010;Orbell et al., 2010).BPV-1 and -2 are closely related serotypes (Shafti-Keramat et al., 2009) and can infect both epithelial and mesenchymal cells. Similar to other PVs, BPV-1/-2 replication and virion production are confined to the epithelial region of the lesions, whilst infection of mesenchymal cells appears to be non-productive (Campo, 2006;Shafti-Keramat et al., 2009).BPV-2 is known to play a central role in bladder carcinogenesis of adult cattle reared on pasturelands rich in bracken fern. In these animals, tumours of the u...

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“…4,8,11,28,30 Furthermore, a potent relationship or co-carcinogenesis has been shown to exist between bovine papillomavirus type 2 (BPV-2) and bracken fern in urinary bladder carcinogenesis in cattle, 10,14,15,28,35 including tumors associated with BEH. The goal of this study was to evaluate different types of bovine urinary bladder tumors (related to BEH) for the presence of the TP53 mutation, using polymerase chain reactionsingle-strand conformation polymorphism (PCR-SSCP) and direct sequencing methods.…”

mentioning

confidence: 99%

TP53 Intronic Mutations in Bovine Enzootic Hematuria–Associated Urinary Bladder Tumors

et al. 2012

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Tumor protein 53 (TP53) is a tumor suppressor gene that is frequently mutated in urinary bladder tumors in both humans and animals. In cattle, urinary bladder tumors have been reported as occurring spontaneously as well as in conjunction with bracken fern consumption-induced bovine enzootic hematuria (BEH). The goal of this study was to evaluate various types of bovine urinary bladder neoplasms for the presence of TP53 alterations, using the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) method. DNA was extracted from both epithelial and mesenchymal urinary bladder tumor samples in cattle, associated with the chronic consumption of bracken fern. PCR was performed using primers targeted to exons 5 to 8, following electrophoresis and isolation, and the products were assessed by SSCP. Tumors in which alterations in the electrophoresis patterns were noted included hemangiomas, papillomas, and carcinomas in situ. Exemplars of these tumor types were selected for sequencing, and although no changes were noted in the 5 to 8 exon range, on either side of the designed primers for exon 6, there was some portion of intron 6 in which sequencing demonstrated a deletion of the thyamine nucleotide at position 9332. In summary, although mutations were not observed within exons 5 to 8, this represents the first report of an intronic mutation in the TP53 gene in association with bovine urinary bladder tumors. Mutations within introns can predispose tissues to the development of cancer, and therefore, a possible association between mutations of the introns of TP53 and the development of urinary bladder tumors in cattle with BEH should be further investigated.

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A Review of Bovine Urothelial Tumours and Tumour-Like Lesions of the Urinary Bladder

Cited by 69 publications

References 76 publications

Bovine papillomavirus: opening new trends for comparative pathology

Bovine papillomavirus: opening new trends for comparative pathology

PBMCs are additional sites of productive infection of bovine papillomavirus type 2

TP53 Intronic Mutations in Bovine Enzootic Hematuria–Associated Urinary Bladder Tumors

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