A Review of CE Detection Methodologies

Swinney, Kelly; Bornhop, Darryl J.

doi:10.1080/10408340091164162

Cited by 32 publications

(13 citation statements)

References 80 publications

(160 reference statements)

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“…LIF is the most sensitive detection mode in CE, CEC, and other microanalytical systems [207,208]. With special designs of detection cells, such as liquid sheath-flow cuvettes and integrated separation and detection devices, LIF detection has a potential to achieve the detection limit of few or even of a single molecule [209][210][211].…”

Section: Lifmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Lifmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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“…Sheath liquid flow rates from 3 to 7 mL/min (3, 5 and 7 mL/min) at different sheath gas flow rates from 5 to 15 arbitrary units (5,8,12, and 15 au) were tested. Low values of sheath gas flow rates produced an unstable spray, so values higher than 8 au were needed.…”

Section: Coupling Conditions Optimizationmentioning

confidence: 99%

“…As regards detection techniques, several approaches have been reported, such as the use of bubble cells [1,2] and Z-shaped cells [3], although in these cases a reduction in resolution is observed. Alternatively, sensitivity can be enhanced with other detection systems, as is the case of charge-coupled device (CCD) array detectors [4], laser-induced fluorescence (LIF) [5,6], or mass spectrometry (MS) [6,7]. Among these, MS has the advantage of being appropriate for a wide number of analytes.…”

Section: Introductionmentioning

confidence: 97%

Determination of heterocyclic aromatic amines by capillary electrophoresis coupled to mass spectrometry using in‐line preconcentration

et al. 2003

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This paper shows the potentiality of capillary electrophoresis (CE) coupled to mass spectrometry (MS) for the analysis of heterocyclic aromatic amines obtaining good results in terms of sensitivity and precision. These compounds have a special interest since they can be carcinogenic for humans. The optimization of a CE-MS method was performed and the best conditions were obtained using a 16 mM formic acid/ammonium formate solution at pH 4.5 with 60% methanol as running electrolyte. For CE-MS coupling, a sheath liquid methanol/20 mM formic acid (75/25) solution at a flow rate of 3 microL/min and hydrodynamic injection of methanol mixtures for 10 s were used. Detection limits ranging from 18 ng/g to 360 ng/g and precisions up to 1.4% and 12% for migration time and concentration, respectively, were obtained. In order to improve sensitivity, field-amplified sample injection was applied as an in-line preconcentration method. Methanol/5 mM formic acid (50/50) as a sample solvent, 3 s hydrodynamic injection (0.5 psi) of a methanol plug, and 25 s of electrokinetic injection (10 kV) of the sample were found to be the optimum conditions. Detection limits up to 25 times lower and similar precisions than those reported for hydrodynamic injection were obtained.

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“…It is well known that UVvisible spectrophotometry, fluorescence spectrometry, electrochemistry, and refractive index detection are the most conventional CE detection methods, while radiometric detection and online NMR are unconventional CE detection methods [1][2][3][4][5]. Electrogenerated chemiluminescence (ECL) is of growing importance in many areas of analytical interest because it can reduce interferences from solution impurities and eliminate the need for complicated construction of postcolumn reactors with low background noise and high detection sensitivity [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Study on the enhancement of Ru(bpy)32+ electrochemiluminescence by nanogold and its application for pentoxyverine detection

et al. 2005

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In this work, CE separation with end-column Ru(bpy)3(2+) ECL detection for the quantitative determination of pentoxyverine was firstly performed. The experimental conditions, such as the applied potential, injection voltage, injection time, and the pH of separation buffer, were discussed in detail. Gold nanoparticles were found to enhance the ECL intensity at an appropriate volume ratio of nanogold with Ru(bpy)3(2+) but without changing their nanoproperties proved by transmission electron microscopy (TEM) and UV-vis spectra. The detection limits with or without nanogold were 6 nM and 0.6 microM, respectively. Successful separation of pentoxyverine, chlorpheniramine, and lidocaine was achieved. This method was also applied to monitor drug binding with HSA, and the binding constant for pentoxyverine was estimated as 1.8 x 10(3)/M.

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A Review of CE Detection Methodologies

Cited by 32 publications

References 80 publications

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Determination of heterocyclic aromatic amines by capillary electrophoresis coupled to mass spectrometry using in‐line preconcentration

Study on the enhancement of Ru(bpy)32+ electrochemiluminescence by nanogold and its application for pentoxyverine detection

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