A Review of Criteria Functions and Response Surface Methodology for the Optimization of Analytical Scale HPLC Separations

Klein, E.; Rivera, S. L.

doi:10.1081/jlc-100100475

Cited by 49 publications

(25 citation statements)

References 24 publications

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“…(ii) The chromatographic functions CRF [10][11][12][13], CRS [10,14], CEF [10,15,16], COF [10,17], ATR [18], Rp [19], and r [20] were calculated according to Eq. (5)- (10) within each group and used as a response in MODDE.…”

Section: Optimization Of Separationmentioning

confidence: 99%

“…Furthermore, different strategies for optimization of the separation were investigated. The compounds were divided into five subgroups and each group was optimized separately by maximizing the selectivity of the critical peak pair using response surface plots in MODDE, by calculating the chromatographic response function (CRF) [10][11][12][13], chromatographic resolution statistic (CRS) [10,14], chromatographic exponential function (CEF) [10,[15][16], chromatographic optimization function (COF) [10,17], arcs tangens resolution (ATR) [18], resolution product (Rp) [19] and relative resolution product (r) [20], and by using the software DryLab  [21][22][23][24][25][26][27]. CRF, CEF, COF, ATR, and r have all been used for optimization of separation in electrodriven techniques.…”

Section: Introductionmentioning

confidence: 99%

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Microemulsion electrokinetic chromatography of drugs varying in charge and hydrophobicity Part II: Strategies for optimization of separation

2004

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The separation of anionic, cationic, and neutral drugs in microemulsion electrokinetic chromatography (MEEKC) was studied. The concentration of sodium dodecyl sulfate (SDS; surfactant) and 2-propanol (organic solvent) was varied in a three-level full factorial design. 29 different model substances were chosen with different hydrophobicities and charges (neutral, positive, and negative). The models were calculated by means of multiple linear regression (MLR). The compounds were divided into five different subgroups, and different strategies for optimization of the separation within each group were investigated. The optimization was done by maximizing the selectivity using response surface plots in MODDE, by calculation of different chromatographic functions, and by using the software DryLab. For all the different groups, MODDE, almost all chromatographic functions and DryLab gave approximately the same settings of the factors for optimum separation. Attempts were made to fit descriptors of the compounds to the retention data from the three-level full factorial design by means of partial least squares projection to latent structures (PLS). Between 86 and 89% of all predictions of migration times were acceptable (80-120% of the observed value).

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Section: Optimization Of Separationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microemulsion electrokinetic chromatography of drugs varying in charge and hydrophobicity Part II: Strategies for optimization of separation

2004

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“…High performance liquid chromatography represents nowadays the most widespread separation technique applied commonly in pharmacology, toxicology, clinical analysis as well as various research fields [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of LC Columns Packed with 2.6 μm Core-Shell and Sub-2 μm Porous Particles for Gradient Separation of Antibiotics

et al. 2011

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The recently introduced Kinetex C18 column packed with core-shell 2.6 lm particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7 lm porous particles. Unlike Acquity BEH C18 column, Kinetex C18 column exhibited lower column backpressure making this column compatible to conventional LC systems. The performance of Kinetex C18 column (2.1 9 50 mm) and Acquity BEH C18 column (2.1 9 50 mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope. The maximal experimental peak capacities for analysis of tetracyclines were 51.8 (Acquity BEH C18 column) and 48.4 (Kinetex C18 column). This indicated that Kinetex C18 was a suitable alternative to Acquity BEH C18 column for the analysis of tetracyclines under acidic conditions. On the contrary, the maximal experimental peak capacities for analysis of macrolides on Acquity BEH C18 column was higher (46.7) than that on Kinetex C18 column (36.9). Moreover, application of Kinetex C18 column for the analysis of macrolides under alkaline conditions was limited with respect to its decreasing performance with growing number of injections on the column.

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“…Methodologies such as response surface analysis (RSA) can help to visualize which are the main experimental variables affecting separation as well as the main interactions between the variables. It models the responses, finds the optimal combination of factors, and it can also predict the most suitable experimental conditions [19][20][21][22]. In the case of HAs, mobile phase pH and buffer composition have shown to be critical parameters for retention factor (k) [23] and, experimental design has also been used for optimization purposes [24].…”

Section: Introductionmentioning

confidence: 99%

Capillary liquid chromatography with diode array and mass spectrometry detection for heterocyclic aromatic amine determination in ready-to-eat food treated with electron-beam irradiation

Gonzalo-Lumbreras

Rosales-Conrado

Pérez-Arribas

et al. 2010

Journal of Chromatography A

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A Review of Criteria Functions and Response Surface Methodology for the Optimization of Analytical Scale HPLC Separations

Cited by 49 publications

References 24 publications

Microemulsion electrokinetic chromatography of drugs varying in charge and hydrophobicity Part II: Strategies for optimization of separation

Microemulsion electrokinetic chromatography of drugs varying in charge and hydrophobicity Part II: Strategies for optimization of separation

Comparison of LC Columns Packed with 2.6 μm Core-Shell and Sub-2 μm Porous Particles for Gradient Separation of Antibiotics

Capillary liquid chromatography with diode array and mass spectrometry detection for heterocyclic aromatic amine determination in ready-to-eat food treated with electron-beam irradiation

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