A REVIEW OF <i>MICROCOCCUS</i> ENTEROTOXIN FOOD POISONING

Haynes, Wm. C.; Hucker, G. J.

doi:10.1111/j.1365-2621.1946.tb16352.x

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…Typical results obtained with some of the culture filtrates are shown in figure 4. All but one of the enterotoxigenic strains gave lines of identity with both toxins.…”

Section: Demonstration Of Antigenicitymentioning

confidence: 92%

Serologic Studies of Staphylococcal Enterotoxin

Ep¹

1958

Public Health Reports (1896-1970)

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'HE NEED for a simple and specific technique for demonstrating and assaying staphylococcal enterotoxin has been recognized for a long time. Research efforts ranging from biological tests with small and relatively cheap animals (1, 2) to extensive chemical and serologic studies of enterotoxin (3) have not attained this important objective.The many contradictory reports found in the literature (4) on staphylococcal enterotoxin are evidence of the inadequacy of the crude, difficult, and impractical tests that are available for its detection. Evidence incriminating suspected foods in outbreaks of staphylococcal food poisoning is largely circumstantial and is limited to the use of epidemiological findings and the demonstration of the presence in the suspected food of appreciable numbers of enterotoxin-producing staphylococci. The very ubiquity of the staphylococcus and, conversely, the possibility of the presence of the heatresistant enterotoxin in foods which no longer contain viable staphylococci, detract considerably from the value of such procedures.Furthermore, the demonstration of enterotoxigenicity of the isolated staphylococcus involves considerable effort. The isolated organism must be cultured on special media in order to produce the enterotoxin, and the presence of the latter is determined by the feeding of monkeys or the parenteral introduction of the culture filtrates into monkeys or cats.

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“…Typical results obtained with some of the culture filtrates are shown in figure 4. All but one of the enterotoxigenic strains gave lines of identity with both toxins.…”

Section: Demonstration Of Antigenicitymentioning

confidence: 92%

Serologic Studies of Staphylococcal Enterotoxin

Ep¹

1958

Public Health Reports (1896-1970)

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“…It can enter as food contaminant and spread its pathogenesis. 10,11 In this case, the first step is to degrade large amount of starch which inhibits bacterial growth. In order to establish itself in this high carbohydrate content S.aureus produces several key enzymes which degrade starch one of them is α-amylase.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of alpha-amylase from a clinical isolate of staphylococcus aureus resistant to vancomycin

Latha¹,

Sushma²,

Sharma³

2020

IJCBR

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Staphylococcus aureus (S. aureus) is one of the prominent gram positive human pathogen secretes many surface and secretary proteins including various enzymes and pathogenic factors that favour the colonization and infection of host tissue. Alpha-amylase is one of the enzymes secreted by S.aureus which catalyses the breakdown of complex sugars to monosaccharides, which are required for colonization and survival of this pathogen. In the present study we have cloned, sequenced, expressed and characterized α-amylase from the clinical isolate of S.aureus resistant to vancomycin (VRSA). The 25kb plasma DNA was isolated from the clinical isolate of S.aureus and a 600bp PCR amplified product was obtained. The SDS-PAGE analysis of induced and un-induced clone SB3 indicates that α-amylase has a M.W. of 22KD. The rα-amylase was eluted from the gel and enzymes assay was performed. The Km was found to be close to Km of native α-amylase identified in the extracellular and in the cytosolic fraction of S.aureus resistant to vancomycin. The current study clearly indicate that there is clear distinction between the α-amylase of human and different prokaryotes, particularly S.aureus which made this enzyme an ideal target in the development of drugs against infections of tooth and oral cavities.

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Studies With Staphylococcal Toxins: I. A Reappraisal of the Validity of the "Kitten Test" as an Indication of Staphylococcal Enterotoxin

Matheson¹,

Thatcher²

1955

Can. J. Microbiol.

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Under controlled conditions of maintenance and treatment of test cats, and with inducement of decisive vomiting as the criterion of enterotoxicity, the "Dolman kitten-test" has provided a high degree of positivity for the detection of an emetic principle in preparations from staphylococcal cultures. These conclusions are based on 315 individual cat injections. Under the conditions of these experiments and with the use of 133 control preparations, false positive reactions likely to cause confusion in normal determinative tests were not encountered.

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A REVIEW OF MICROCOCCUS ENTEROTOXIN FOOD POISONING

Cited by 3 publications

References 35 publications

Serologic Studies of Staphylococcal Enterotoxin

Serologic Studies of Staphylococcal Enterotoxin

Cloning and characterization of alpha-amylase from a clinical isolate of staphylococcus aureus resistant to vancomycin

Studies With Staphylococcal Toxins: I. A Reappraisal of the Validity of the "Kitten Test" as an Indication of Staphylococcal Enterotoxin

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