A review of manufacturing capabilities of cell spheroid generation technologies and future development

Liú, Dan; Chen, Sixun; Naing, May Win

doi:10.1002/bit.27620

Cited by 55 publications

(44 citation statements)

References 99 publications

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“…Microfluidic large-scale integration (mLSI) enables the automation of cell culture processes in a highly parallel manner, with precise spatiotemporal control of the cellular microenvironment. [1][2][3] mLSI platforms have been utilized for various cell culture processes, including high-throughput single-cell analysis, 4 standardized stem cell differentiation, 5 protein signaling analysis, 6 conditional transcriptomics, 7 and cell-to-cell interaction analysis. 8 To date, the majority of mLSI applications utilize traditional two-dimensional (2D) monolayer cell cultures, which provide robust, cost-effective, and repeatable access to cell screening assays.…”

Section: Introductionmentioning

confidence: 99%

Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip

et al. 2021

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“…Spheroids can be derived from either established cell lines or primary isolated tumor cells [ 35 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 ]. Usually, spheroids are obtained culturing tumor cells in ultra-low adherent plasticware or by hanging drop methods, forcing tumor cells to aggregate and generate tumor sphere in static or microfluidic systems [ 15 , 16 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 ].…”

Section: Tumor Cell Spheroids As a 3d Model To Study Tumor Cell Biology And Tumor-natural Killer Cross-talkmentioning

confidence: 99%

Three-Dimensional Culture Models to Study Innate Anti-Tumor Immune Response: Advantages and Disadvantages

Poggi

Villa

Fernadez

et al. 2021

Cancers

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Several approaches have shown that the immune response against tumors strongly affects patients’ clinical outcome. Thus, the study of anti-tumor immunity is critical to understand and potentiate the mechanisms underlying the elimination of tumor cells. Natural killer (NK) cells are members of innate immunity and represent powerful anti-tumor effectors, able to eliminate tumor cells without a previous sensitization. Thus, the study of their involvement in anti-tumor responses is critical for clinical translation. This analysis has been performed in vitro, co-incubating NK with tumor cells and quantifying the cytotoxic activity of NK cells. In vivo confirmation has been applied to overcome the limits of in vitro testing, however, the innate immunity of mice and humans is different, leading to discrepancies. Different activating receptors on NK cells and counter-ligands on tumor cells are involved in the antitumor response, and innate immunity is strictly dependent on the specific microenvironment where it takes place. Thus, three-dimensional (3D) culture systems, where NK and tumor cells can interact in a tissue-like architecture, have been created. For example, tumor cell spheroids and primary organoids derived from several tumor types, have been used so far to analyze innate immune response, replacing animal models. Herein, we briefly introduce NK cells and analyze and discuss in detail the properties of 3D tumor culture systems and their use for the study of tumor cell interactions with NK cells.

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“…Cells are aggregated into a spheroid and cultured in suspension. Various spheroid fabrication methods such as hanging drop, non-adherent culture plate, gel embedding, magnetic levitation, and spinner culture have been reported ( 11 , 14 - 16 ). It has been demonstrated that cell spheroids exhibit improved cellular functions in vitro as compared to a monolayer culture ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

Biomaterials-assisted spheroid engineering for regenerative therapy

Lee

Bayaraa

Zechu

et al. 2021

BMB Rep

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Cell-based therapy is a promising approach in the field of regenerative medicine. As cells are formed into spheroids, their survival, functions, and engraftment in the transplanted site are significantly improved compared to single cell transplantation. To improve the therapeutic effect of cell spheroids even further, various biomaterials (e.g., nano- or microparticles, fibers, and hydrogels) have been developed for spheroid engineering. These biomaterials not only can control the overall spheroid formation (e.g., size, shape, aggregation speed, and degree of compaction), but also can regulate cell-to-cell and cell-to-matrix interactions in spheroids. Therefore, cell spheroids in synergy with biomaterials have recently emerged for cell-based regenerative therapy. Biomaterials-assisted spheroid engineering has been extensively studied for regeneration of bone or/and cartilage defects, critical limb ischemia, and myocardial infarction. Furthermore, it has been expanded to pancreas islets and hair follicle transplantation. This paper comprehensively reviews biomaterials-assisted spheroid engineering for regenerative therapy.

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A review of manufacturing capabilities of cell spheroid generation technologies and future development

Cited by 55 publications

References 99 publications

Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip

Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip

Three-Dimensional Culture Models to Study Innate Anti-Tumor Immune Response: Advantages and Disadvantages

Biomaterials-assisted spheroid engineering for regenerative therapy

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