A review of microcystin detections in Estuarine and Marine waters: Environmental implications and human health risk

Preece, Ellen P.; Hardy, F. Joan; Moore, Barry; Bryan, Michael D.

doi:10.1016/j.hal.2016.11.006

Cited by 302 publications

(218 citation statements)

References 143 publications

Supporting

Mentioning

196

Contrasting

Unclassified

Order By: Relevance

“…The variability of the tolerance of different strains of M. aeruginosa to salinity is surprising (from 1 to 14 g L −1 , Preece et al . ). Our results showed that the strain MaAVc of M. aeruginosa can adapt up to 10 g L −1 , which is within the range of tolerance that has been observed in other strains of M. aeruginosa (Preece et al .…”

Section: Discussionmentioning

confidence: 97%

“…Our results showed that the strain MaAVc of M. aeruginosa can adapt up to 10 g L −1 , which is within the range of tolerance that has been observed in other strains of M. aeruginosa (Preece et al . ). However, in the fluctuation analysis experiment when the cells were cultured at a lethal dose of NaCl (14 g L −1 ), although they became bleached initially, after some days in these conditions the growth of resistant cells was detected.…”

Section: Discussionmentioning

confidence: 97%

“…However, several strains of M. aeruginosa can tolerate salinities ≤10 g NaCl L −1 , up to 14 g NaCl L −1 (Preece et al . ). It must be highlighted that previous studies on the effect of increased salinity on M. aeruginosa assessed the physiological or biochemical responses of cells after only a few generations under the experimental conditions (Otsuka et al .…”

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Adaptation of the toxic freshwater cyanobacterium Microcystis aeruginosa to salinity is achieved by the selection of spontaneous mutants

Melero‐Jiménez

Martín‐Clemente

García‐Sánchez

et al. 2019

Phycological Research

View full text Add to dashboard Cite

SUMMARY The cyanobacterium Microcystis aeruginosa causes most of the harmful toxic blooms in freshwater ecosystems. Some strains of M. aeruginosa tolerate low‐medium levels of salinity, and because salinization of freshwater aquatic systems is increasing worldwide it is relevant to know what adaptive mechanisms allow tolerance to salinity. The mechanisms involved in the adaptation of M. aeruginosa to salinity (acclimation vs. genetic adaptation) were tested by a fluctuation analysis design, and then the maximum capacity of adaptation to salinity was studied by a ratchet protocol experiment. Whereas a dose of 10 g NaCl L−1 completely inhibited the growth of M. aeruginosa, salinity‐resistant genetic variants, capable of tolerating up to 14 g NaCl L−1, were isolated in the fluctuation analysis experiment. The salinity‐resistant cells arose by spontaneous mutations at a rate of 7.3 × 10−7 mutants per cell division. We observed with the ratchet protocol that three independent culture populations of M. aeruginosa were able to adapt to up to 15.1 g L−1 of NaCl, suggesting that successive mutation‐selection processes can enhance the highest salinity level to which M. aeruginosa cells can initially adapt. We propose that increasing salinity in water reservoirs could lead to the selection of salinity‐resistant mutants of M. aeruginosa.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Adaptation of the toxic freshwater cyanobacterium Microcystis aeruginosa to salinity is achieved by the selection of spontaneous mutants

Melero‐Jiménez

Martín‐Clemente

García‐Sánchez

et al. 2019

Phycological Research

View full text Add to dashboard Cite

show abstract

“…For example, rainfall from urban habitats brings large concentrations of human pathogenic bacteria and viruses into coastal waters (Noble et al, 2006). Recently, freshwater microcystins have been associated with mortality in sea otter populations in central California (Miller et al, 2010), and incubation of several invertebrates in aquaria containing microcystin resulted in biological accumulation of microcystins (Preece et al, 2017). We sought to examine concentrations of microcystins in asteroid tissues experiencing SSWD from October 2013 in the Puget Sound.…”

Section: How Did Mass Mortality Correspond With Environmental Conditimentioning

confidence: 99%

Investigating the Complex Association Between Viral Ecology, Environment, and Northeast Pacific Sea Star Wasting

et al. 2018

View full text Add to dashboard Cite

Sea Star Wasting Disease (SSWD) describes a suite of disease signs that affected >20 species of asteroid since 2013 along a broad geographic range from the Alaska Peninsula to Baja California. Previous work identified the Sea Star associated Densovirus (SSaDV) as the best candidate pathogen for SSWD in three species of common asteroid (Pycnopodia helianthoides, Pisaster ochraceus, and Evasterias troscheli), and virus-sized material (<0.22 µm) elicited SSWD signs in P. helianthoides. However, the ability of virus-sized material to elicit SSWD in other species of asteroids was not known. Discordance between detection of SSaDV by qPCR and by viral metagenomics inspired the redesign of qPCR primers to encompass SSaDV and two densoviral genotypes detected in wasting asteroids. Analysis of asteroid samples collected during SSWD emergence in 2013-2014 showed an association between wasting asteroid-associated densoviruses (WAaDs) and SSWD in only one species (P. helianthoides). WAaDs were found in association with asymptomatic asteroids in contemporary (2016 and later) populations, suggesting that they may form subclinical infections at the times they were sampled. WAaDs were found in SSWD-affected P. helianthoides after being absent in asymptomatic individuals a year earlier at one location (Kodiak). Direct challenge of P. ochraceus, Pisaster brevispinus, and E. troscheli with virus-sized material from SSWD-affected individuals did not elicit SSWD in any trial. RNA viral genomes discovered in viral metagenomes and host transcriptomes had viral loads and metagenome fragment recruitment patterns that were inconsistent with SSWD. Analysis of water temperature and precipitation patterns on a regional scale suggests that SSWD occurred following dry conditions at several locations, but mostly was inconsistently associated with either parameter. Semi-continuous monitoring of SSWD subtidally at two sites in the Salish Sea from 2013 to 2017 indicated that SSWD in E. troscheli and P. ochraceus was associated with elevated water temperatures, but wasting in P. helianthoides occurred irrespective of environmental conditions. Our data therefore do not support that widespread SSWD is associated with potential viral pathogens in species other than P. helianthoides. Rather, we speculate that SSWD may represent a syndrome of heterogeneous etiologies between geographic locations, between species, or even within a species between locations.

show abstract

“…Industrialization and urbanization have led to a substantial amount of industrial and domestic wastewater being discharged into natural water resources worldwide, resulting in increasingly serious levels of eutrophication . Cyanobacteria (also known as Cyanophyceae ) are photosynthetic organisms that can proliferate in marine and freshwater environments with high loads of nutrients (e. g., nitrogen and phosphorus compounds), attributable to anthropogenic enrichment .…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

et al. 2018

View full text Add to dashboard Cite

Microcystins are potent hepatotoxins produced by cyanobacteria, which proliferate in wastewaters with high nutrient content. Due to their high toxicity and potential risk to human health, even at low concentrations, the development of a sensitive and rapid method for the monitoring of microcystin‐LR (MC‐LR) in water samples is of great importance. In this context, a new direct electrochemical nano‐immunosensor for MC‐LR detection using the liquid crystal (E)‐1‐decyl‐4‐[(4‐decyloxyphenyl)diazenyl]pyridinium bromide (Br‐Py) as a redox probe and gold nanoparticles stabilized in bovine serum albumin (AuNP‐BSA) is described herein. The microcystin‐LR antibody (anti‐MC‐LR) was covalently immobilized using N‐(3‐dimethylaminopropyl)‐N‐ethylcarbodiimide hydrochloride (EDC) and N‐hydroxysuccinimide (NHS) on an AuNP‐BSA/BrPy film. The proposed sensor response is based on the inhibition of the Br‐Py electrochemical signal after the specific interaction of MC‐LR with immobilized anti‐MC‐LR on the electrode surface. The electrochemical behavior of the immunosensor was studied by square‐wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Under optimized conditions, using SWV and an incubation time of 15 min, the immunosensor exhibits a linear response to MC‐LR concentrations of 0.05 to 500.0 ng mL−1 with a detection limit of 0.05 ng mL−1. The anti‐MC‐LR/AuNP‐BSA/Br‐Py/GCE was successfully applied in the determination of MC‐LR in spiked seawater samples.

show abstract

A review of microcystin detections in Estuarine and Marine waters: Environmental implications and human health risk

Cited by 302 publications

References 143 publications

Adaptation of the toxic freshwater cyanobacterium Microcystis aeruginosa to salinity is achieved by the selection of spontaneous mutants

Adaptation of the toxic freshwater cyanobacterium Microcystis aeruginosa to salinity is achieved by the selection of spontaneous mutants

Investigating the Complex Association Between Viral Ecology, Environment, and Northeast Pacific Sea Star Wasting

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

Contact Info

Product

Resources

About