A review of molecular biology detection methods for human adenovirus

Abstract

<abstract> <p>In humans particularly in children, adenovirus is one of the most common viruses that cause respiratory illnesses. Knowing how to detect adenovirus proficiently and rapidly will help reinforce surveillance of adenovirus infections, detect epidemic situations in real-time, and understand the trend of virus epidemics, which will allow effective actions to be taken quickly. The rapid detection of antiviral antibodies or viral antigens in clinical samples can be achieved by molecular diagnostic techniques like PCR, Real-Time PCR, LAMP, mPCR-RLB, PCR-ELISA, Tem-PCR, Gene Chip, and so on. Some of the molecular diagnostic methods are relatively economical, exceedingly sensitive and explicit. There are several commercially accessible molecular diagnostic techniques that enable their use in clinical laboratories all over the world. In this review, the principles, characteristics, and applications of molecular biology surveillance methods commonly used in labs and clinics for the detection of human adenoviruses are examined and highlighted.</p> </abstract>

Unlocking the Power of Molecular Cloning: Revolutionizing Medical Microbiology Procedures

“…It involves theuseofmultipleprimersetsthatspecificallybindtoconservedregionsofthe microbialgenome (Datta,2023).TheseprimersareusedinamultiplexPCRreaction to amplify multiple target DNA sequences simultaneously. The amplified DNAisthenhybridizedtoareverselineblotmembranecontainingprobesfor differentmicrobes.Thepresenceorabsenceofhybridizationsignalsallowsfor theidentificationofspecificmicrobialtypesinthesample.Tem-PCRcombines the specificity of PCR with target enrichment to detect and identify microbes involved in polymicrobial infections (Datta, 2023). The target enrichment step involvestheselectiveamplificationofspecificregionsofinterestfromthesample DNA using specific probes.…”