A Review of Molecular Mechanisms of the Anti-Leukemic Effects of Phenolic Compounds in Honey

Abubakar, Murtala Bello; Abdullah, Wan Zaidah; Sulaiman, Siti Amrah; Suen, Ang Boon

doi:10.3390/ijms131115054

Cited by 79 publications

(50 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The disruption of the normal cell cycle plays a vital role in the development of cancer. A large number of anticancer natural compounds have been shown to induce cell death and apoptosis in close association with cell cycle arrest at the G1 phase 9 (24)(25)(26). In this study, Jurkat and CEM-CM3 cells treated with CGE showed a significant accumulation of cells in the G1 phase; there was a concurrent reduction of the cell population in S and G2/M phase in a dose-dependent manner (Fig.…”

Section: Discussionmentioning

confidence: 52%

Centipedegrass extract induces apoptosis through the activation of caspases and the downregulation of PI3K/AKT and MAPK phosphorylation in leukemia cells

Bai

Badaboina

Park

et al. 2014

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Acute lymphoblastic leukemia (ALL), which involves the blood and bone marrow, is the most common type of cancer in children younger than 5 years of age. Previous studies have investigated the effects of centipedegrass extract (CGE), which is mainly composed of maysin and its derivatives, and have demonstrated that it has various biological activities, including antioxidant and anti‑inflammatory activities, pancreatic lipase inhibitory activity, anti-adipogenic activity and insecticidal activity. To the best of our knowledge, this study is the first to investigate the anticancer effects of CGE in ALL cell lines and to elucidate the mechanisms underlying these effects. Cell viability was measured by thiazolyl blue tetrazolium blue (MTT) assay. Apoptosis, cell cycle progression and mitochondrial membrane potential (∆Ψm) were determined by flow cytometry. The effects of CGE on the phosphatidylinositol 3‑kinase (PI3K)/Akt pathway and mitogen‑activated protein kinases (MAPKs) were assessed by immunoblotting. PI3K, MAPK and caspase inhibitors were used to further confirm the molecular mechanisms involved. Our results clearly demonstrated that the proliferation of the ALL cells was significantly inhibited by CGE in a dose‑dependent manner. Apoptosis was accompanied by the induction of significant G1 cell cycle arrest. The resulting alteration of the ∆Ψm increased the activity of caspase‑3/7. The induction of apoptosis was enhanced by the combined treatment of CGE with a PI3K inhibitor or an extracellular signal-regulated kinase (ERK) inhibitor, whereas the CGE‑induced apoptosis was inhibited in the presence of caspase inhibitors, such as z‑VAD‑fmk and z‑IETD‑fmk. Furthermore, CGE inhibited PI3K activity by decreasing the levels of phosphorylated (p‑)Akt, p‑BAD, and Bcl‑2 together with the levels of MAPKs, including p‑ERK and p‑JNK, but demonstrated no effects on p38 MAPK. Thus, our data suggest that CGE may be a novel natural compound with potential for use as an antitumor agent in ALL.

show abstract

Section: Discussionmentioning

confidence: 52%

Centipedegrass extract induces apoptosis through the activation of caspases and the downregulation of PI3K/AKT and MAPK phosphorylation in leukemia cells

Bai

Badaboina

Park

et al. 2014

International Journal of Molecular Medicine

View full text Add to dashboard Cite

show abstract

“…We have recently reviewed the origin and characteristics of HL-60, U937, MOLT-4, NALM-6, and K562 cell lines [75]. HL-205 cells are clones of cells isolated from HL-60 cells [94].…”

Section: Introductionmentioning

confidence: 99%

Polyphenols as Key Players for the Antileukaemic Effects of Propolis

Abubakar

Abdullah

Sulaiman

et al. 2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Propolis (a bee product) which has a long history of medicinal use by humans has attracted a great deal of research interest in the recent time; this is due to its widely reported biological activities such as antiviral, antifungal, antibacterial, anti-inflammatory, antioxidant, and anticarcinogenic properties. Crude form of propolis and its phenolic contents have both been reported to exhibit antileukaemic effects in various leukaemia cell lines. The ability of the polyphenols found in propolis to arrest cell cycle and induce apoptosis and differentiation in addition to inhibition of cell growth and proliferation makes them promising antileukaemic agents, and hence, they are believed to be a key to the antileukaemic effects of propolis in different types of leukaemia. This paper reviews the molecular bases of antileukaemic activity of both crude propolis and individual polyphenols on various leukaemia cell lines, and it indicates that propolis has the potential to be used in both treatment and prevention of leukaemia. This however needs further evaluation by in vitro, in vivo, and epidemiological studies as well as clinical trials.

show abstract

“…Inspite of their significant difference in their phenolic content, % T/C values show barely discernible difference between the two honey samples. Researchers had identified some phenolic components of honey that have been attributed to the antileukemic activity [ 11 ]. Hence, we assume that qualitative nature of phenolic constituents present in the honey may play a vital role against the leukemia but not the total phenolic content of honey.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Selected Honey and One of Its Phenolic Constituent Eugenol against L1210 Lymphoid Leukemia

Jaganathan

Mondhe

Wani

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

People affected with leukemia are on the rise and several strategies were employed to thwart this deadly disease. Recent decade of research focuses on phenolic constituents as a tool for combating various inflammatory, cancer, and cardiac diseases. Our research showed honey and its phenolic constituents as crusaders against cancer. In this work, we explored the antileukemic activity of selected honey and one of its phenolic constituent eugenol against L1210 leukemia animal model. Results of this experiment showed that the selected honey samples as well as eugenol after intraperitoneal injection could not increase the median survival time (MST) of animals. Further, there was only slight marginal increase in the %T/C values of honey and eugenol treated groups. The number of phenolics present in the honey may not be a prime factor to promote antileukemic effect since there was no difference in the MST of two different honeys tested. This study limits the use of selected honey and eugenol against leukemia animal model.

show abstract

A Review of Molecular Mechanisms of the Anti-Leukemic Effects of Phenolic Compounds in Honey

Cited by 79 publications

References 82 publications

Centipedegrass extract induces apoptosis through the activation of caspases and the downregulation of PI3K/AKT and MAPK phosphorylation in leukemia cells

Centipedegrass extract induces apoptosis through the activation of caspases and the downregulation of PI3K/AKT and MAPK phosphorylation in leukemia cells

Polyphenols as Key Players for the Antileukaemic Effects of Propolis

Evaluation of Selected Honey and One of Its Phenolic Constituent Eugenol against L1210 Lymphoid Leukemia

Contact Info

Product

Resources

About