A Review of Nanobioscience and Bioinformatics Initiatives in North America

Oakley, Barbara; Hanna, Darrin M.

doi:10.1109/tnb.2003.820259

Cited by 6 publications

(9 citation statements)

References 114 publications

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“…Biotin-MBP was immobilized in column 1, as a positive control, since our previous studies have shown that this protein can link MBP-QD-avidin to a NeutrAvidin surface. 28 Data in parts A and B of Figure 6 clearly show that the biotinfunctionalized CPMV HIS mutants bind MBP-QD-avidin with high specificity, as evident by the discrete bright squares obtained in patterning columns [3] and [4]. However the MBP-QD-avidin was not found to interact with the NeutrAvidin surface alone, as shown in the PBS column [2] of parts A and B of Figure 6, demonstrating the importance of the biotin-functionalized CPMV HIS in the specific immobilization of the MBP-QD-avidin.…”

Section: Resultsmentioning

confidence: 95%

“…Here, CPMV immobilization used either Scheme 2 or a combination of Scheme 1 and Scheme 2; see Scheme 3 Figure 7, on the same slide. Biotin-CPMV-EF HIS particles were immobilized either via interactions of the biotin functions with NeutrAvidin on the surface (Figure 7, column [2]) or via interactions of the HIS functions with NTA (in the biotin-X-NTA), previously attached to Neu-trAvidin on the substrates (Figure 7, column [4]). Again, the MBP-QD-avidin was not found to interact with the NeutrAvidin surface alone, as shown in the PBS column [1] Figure 7, nor was it found to interact with the biotin-X-NTA-Ni 2+ functionalized region of the slide, column [3].…”

Section: Resultsmentioning

confidence: 99%

“…These properties could be utilized for driving the signal transduction necessary for creating molecular scale diagnostic or sensing devices. One of the goals in designing these hybrids is to create new types of functional nanoscale devices, such as sensors. − Rather than using lengthy schemes for synthesizing and purifying these hybrid materials, which can be further complicated by the labile nature of biomolecules, it would be simpler to exploit the programmable properties of the biological systems along with their ability to self-assemble to form these new nanostructures. Due to its base-pairing complementarily, DNA has long been used in creating a variety of multifunctional hybrid organic/inorganic nanostructures, such as hybrid nanowires and transistors. ,, However, DNA has only four naturally occurring bases, which limits the number of possible combinations and types of available chemical interactions.…”

Section: Introductionmentioning

confidence: 99%

“…One of the goals in designing these hybrids is to create new types of functional nanoscale devices, such as sensors. [1][2][3][4] Rather than using lengthy schemes for synthesizing and purifying these hybrid materials, which can be further complicated by the labile nature of biomolecules, it would be simpler to exploit the programmable properties of the biological systems along with their ability to self-assemble to form these new nanostructures. Due to its base-pairing complementarily, DNA has long been used in creating a variety of multifunctional hybrid organic/inorganic nanostructures, such as hybrid nanowires and transistors.…”

Section: Introductionmentioning

confidence: 99%

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Decoration of Discretely Immobilized Cowpea Mosaic Virus with Luminescent Quantum Dots

et al. 2005

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This report describes two related methods for decorating cowpea mosaic virus (CPMV) with luminescent semiconductor nanocrystals (quantum dots, QDs). Variants of CPMV are immobilized on a substrate functionalized with NeutrAvidin using modifications of biotin-avidin binding chemistry in combination with metal affinity coordination. For example, using CPMV mutants expressing available 6-histidine sequences inserted at loops on the viral coat protein, we show that these virus particles can be specifically immobilized on NeutrAvidin functionalized substrates in a controlled fashion via metal-affinity coordination. To accomplish this, a hetero-bifunctional biotin-NTA moiety, activated with nickel, is used as the linker for surface immobilization of CPMV (bridging the CPMVs' histidines to the NeutrAvidin). Two linking chemistries are then employed to achieve CPMV decoration with hydrophilic CdSe-ZnS core-shell QDs; they target the histidine or lysine residues on the exterior virus surface and utilize biotin-avidin interactions. In the first scheme, QDs are immobilized on the surface-tethered CPMV via electrostatic attachment to avidin previously bound to the virus particle. In the second strategy, the lysine residues common to each viral surface asymmetric unit are chemically functionalized with biotin groups and the biotinylated CPMV is discretely immobilized onto the substrate via NeutrAvidin-biotin interactions. The biotin units on the upper exposed surface of the immobilized CPMV then serve as capture sites for QDs conjugated with a mixture of avidin and a second protein, maltose binding protein, which is also used for QD-protein conjugate purification. Characterization of the assembled CPMV and QD structures is presented, and the potential uses for protein-coated QDs functionalized onto this symmetrical virion nanoscaffold are discussed.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Decoration of Discretely Immobilized Cowpea Mosaic Virus with Luminescent Quantum Dots

et al. 2005

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“…Angel investors, venture capitalists and corporate funds are mostly offered through supportive entities such as incubators, accelerators, small business development centers and hatcheries. Problems in financing startups are often centered on the fact that many of the best and potentially useful technology applications are a decade or more away from becoming commercial products (Oakley and Hanna, 2004). But startups aim to grow rapidly.…”

Section: The Challengesmentioning

confidence: 99%

The enterprising communities and startup ecosystem in Iran

Salamzadeh

Kawamorita

2017

JEC

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Purpose Startups play a significant role in improving societies. This paper concentrates on the concept of “startups” and attempts to present a more comprehensive view of this phenomenon. Also, the focus of this paper is on investigating enterprising communities and the startup ecosystem in Iran. Design/methodology/approach The existing literature is carefully reviewed, definition and views are explored and the story of this phenomenon is told from the formation to the exit stage. The author elaborates the existing stages and challenges of startups in Iran. To do so, 65 tech startup founders are interviewed. Semi-structured interviews (SI) were based on previous studies mentioning the lifecycle of the startups, and were fully recorded. Moreover, secondary sources of data (SS) were used to support the findings. Findings The paper classifies the extant theories of startups in two levels, i.e. macro level, and micro and meso levels. Also, it defines some steps for the formation of startups and studies the Iranian startup ecosystem. Last but not the least the paper contributes to the startup ecosystem of Iran which is changing disruptively, especially in the last couple of years. Research limitations/implications Findings of this research shed light on the existing ecosystem of startups in Iran, which is rarely studied in previous research. Lack of enough evidence in the existing literature of startup ecosystem in Iran was the most significant limitation of the research which increased the need for more investigation and elaboration. Originality/value The paper contributes to understanding the startup ecosystem in Iran, and the challenges.

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Molecular Bioengineering and Nanobioscience: Data Analysis and Processing Methods

Cerutti¹,

Marchesi²

2011

Advanced Methods of Biomedical Signal Processing

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A Review of Nanobioscience and Bioinformatics Initiatives in North America

Cited by 6 publications

References 114 publications

Decoration of Discretely Immobilized Cowpea Mosaic Virus with Luminescent Quantum Dots

Decoration of Discretely Immobilized Cowpea Mosaic Virus with Luminescent Quantum Dots

The enterprising communities and startup ecosystem in Iran

Molecular Bioengineering and Nanobioscience: Data Analysis and Processing Methods

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