Decoration of Discretely Immobilized Cowpea Mosaic Virus with Luminescent Quantum Dots

Medintz, Igor L.; Sapsford, Kim E.; Konnert, J. H.; Chatterji, Anju; Lin, Tianwei; Johnson, John E.; Mattoussi, Hedi

doi:10.1021/la0468287

Cited by 99 publications

(84 citation statements)

References 44 publications

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“…1). 7,19,20 An AFM image of a CPMV film showing the pseudo-three-fold capsid geometry and its relation to CPMV molecular structure is given in Fig. 1d.…”

Section: Resultsmentioning

confidence: 99%

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Physical Controls on Directed Virus Assembly at Nanoscale Chemical Templates

et al. 2006

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Viruses are attractive building blocks for nanoscale heterostructures, but little is understood about the physical principles governing their directed assembly. In-situ force microscopy was used to investigate organization of Cowpea Mosaic Virus engineered to bind specifically and reversibly at nanoscale chemical templates with sub-30nm features. Morphological evolution and assembly kinetics were measured as virus flux and inter-viral potential were varied. The resulting morphologies were similar to those of atomic-scale epitaxial systems, but the underlying thermodynamics was analogous to that of colloidal systems in confined geometries. The 1D templates biased the location of initial cluster formation, introduced asymmetric sticking probabilities, and drove 1D and 2D condensation at subcritical volume fractions. The growth kinetics followed a t 1/2 law controlled by the slow diffusion of viruses. The lateral expansion of virus clusters that initially form on the 1D templates following introduction of polyethylene glycol (PEG) into the solution suggests a significant role for weak

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“…1). 7,19,20 An AFM image of a CPMV film showing the pseudo-three-fold capsid geometry and its relation to CPMV molecular structure is given in Fig. 1d.…”

Section: Resultsmentioning

confidence: 99%

“…1a). 20 We chose the Ni-NTA linker because it is a well-known immunolabeling compound for poly-His tagged biomolecules. [23][24][25][26][27] Its small size compared to more commonly used antigen-antibody or a streptavidin-biotin pairs makes it ideal for efficiently localizing binding sites at molecular resolution with little steric hindrance.…”

Section: Resultsmentioning

confidence: 99%

Physical Controls on Directed Virus Assembly at Nanoscale Chemical Templates

et al. 2006

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“…In another example, surface-modified CPMV capsids were patterned on glass surfaces and subsequently decorated with semiconductor quantum dots (QDs). [121] The columns on which CPMV was immobilized prior to decoration with quantum dots produce a 75 % larger fluorescence signal than those on which quantum dots are immobilized directly. These results indicate that exterior surfaces of protein cages can be exploited as scaffolds for loading large numbers of molecules because of their multivalent functional-group presentation and very large surface area.…”

Section: Icosahedral Viral Capsids: Exteriormentioning

confidence: 99%

Biological Containers: Protein Cages as Multifunctional Nanoplatforms

Uchida¹,

Klem²,

Allen³

et al. 2007

Advanced Materials

535

518

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Materials scientists increasingly draw inspiration from the study of how biological systems fabricate materials under mild synthetic conditions by using self‐assembled macromolecular templates. Containerlike protein architectures such as viral capsids and ferritin are examples of such biological templates. These protein cages have three distinct interfaces that can be synthetically exploited: the interior, the exterior, and the interface between subunits. The subunits that comprise the building blocks of these structures can be modified both chemically and genetically in order to impart designed functionality to different surfaces of the cage. Therefore, the cages possess a great deal of synthetic flexibility, which allows for the introduction of multifunctionality in a single cage. In addition, hierarchical assembly of the functionalized cages paves the way for development of a new class of materials with a wide range of applications from electronics to biomedicine.

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“…Quantum dots: Quantum dots (QD) became powerful tools to improve molecular imaging and diagnostics as a result of their unique photophysical properties [15][16][17]. The unique features of QD are resistance to photo bleaching high signal intensity, fluorescence resonance energy transfer (FRET), and enhanced binding affinity to the target.…”

Section: Liposomes and Micellementioning

confidence: 99%

Approaches in the Chemoprevention of Breast Cancer

Kelly¹

2013

J Cancer Sci Ther

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Nanotechnology in bioscience and therapeutics has advanced tremendously in this decade. Many nanoparticle formulations as well as passive and active targeted agents have been developed and proved effective preclinically in proof-of-concept models of different cancers.Many of these formulations use polymer nanoparticles, liposomes, bubkyballs, fullerenes, carbon nanotubes, dendrimers, isotope tags, and PEG compounds. In effect, nanoparticle formulations have become the norm in chemoprevention. In this paper we review nanoformulations of bioactive compounds that have been tested for their potential mechanistic antiproliferative activity in breast cancer. We also discuss the possibility of enhancing the activity of these compounds using different innovative bioconjugation methods. Citation: Dileep KV, Kelly M, Hardin E, Sadasivan C, Nair HB (2013) Approaches in the Chemoprevention of Breast Cancer. J Cancer Sci Ther 5: 282-288. doi:10.4172/1948-5956.1000217 Volume 5 number of bound reporter metal atoms per vesicle and decrease dosage without compromising signal intensity. Adding specific targeted moieties to the liposomes also could improve the targeting of contrast agents designed for breast cancer imaging. Another research group has recently reported monoclonal antibody to 2C5 (mAb2C5) that can specifically detect tumor cells, leaving the normal cells behind [9]. It is claimed that these liposomes are capable of specifically delivering diagnostic moieties to various tumors with short image acquisition time. Another group has showed PEGylated immunoliposomes labeled with 99 Tc, surface conjugated with F fragment of GAH mAb, could target human gastric cancer in a murine model [10]. Some other studies investigating immunotargeted liposomes for MR imaging enhanced their contrast using gadopentetate dimeglumine (Gd-complex) [11]. MRI signal amplification is also achieved by combining PAP and Gd moietiesin mAb2C5-modified antibodies [12]. Superior in vivo tumor accumulation of mAb2C5 in mouse models of murine Lewis lung carcinoma has been achieved by modified PAP-containing pegylated liposomes, enabling faster detection of the solid tumors [13]. Use of primary and secondary antibodies for increasing the contrast has also been tested. Typically in this inverse imaging technique, patients will receive first antitumor antibody systemically for tumor imaging. This is followed by a second antibody, encapsulated in liposomes directed against the first antibody, which enhances reticuloendothelial (RES) clearance of the first antibody, enabling distinction between normal and tumor tissues [14]. This technology has stability issues with its secondary antibody and needs to be further refined for clinical diagnostic use. Approaches in the Chemoprevention of Breast Cancer Journal ofQuantum dots: Quantum dots (QD) became powerful tools to improve molecular imaging and diagnostics as a result of their unique photophysical properties [15][16][17]. The unique features of QD are resistance to photo bleaching high signal ...

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Decoration of Discretely Immobilized Cowpea Mosaic Virus with Luminescent Quantum Dots

Cited by 99 publications

References 44 publications

Physical Controls on Directed Virus Assembly at Nanoscale Chemical Templates

Physical Controls on Directed Virus Assembly at Nanoscale Chemical Templates

Biological Containers: Protein Cages as Multifunctional Nanoplatforms

Approaches in the Chemoprevention of Breast Cancer

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