Background and aims Competitive vegetation in forest stands in uence seedling growth by changing soil nutrient availability. However, studies on the effects of different weed control methods on seedling growth of Chinese r (Cunninghamia lanceolata (Lamb.) Hook.) are rare.Methods We applied three weed control methods, comprising arti cial sickle weeding (ASW), woody disc weeding (WDW), and nonwoven cloth weeding (nWCW), to explore their effect on growth of Chinese r seedlings in a plantation in Jiangxi Province, China.
ResultsThe weed control methods affected the shoot height and root-collar diameter of the seedlings. The contents of sugar, glucose, triglycerides, total cholesterol, and free fatty acids in newly developed leaves were increased after ASW and nWCW treatment, and were consistent with the expression of genes associated with glucokinase, sucrose phosphate synthase, and sucrose synthase.Weeding method in uenced soil properties, including pH, moisture, total nitrogen (N), ammonium-N, nitrate-N, total phosphorus, available phosphorus, and dissolved organic carbon contents. Moisture content was the main factor that in uenced the soil bacterial community and leaf nutrition. High-throughput sequencing of the bacterial 16S rRNA gene revealed that the weeding methods affected bacterial community structure. Speci cally, compared with ASW and nWCW, WDW contributed to lower soil bacterial diversity, simpler bacterial interaction, and increase in pathogenic bacteria potential.Conclusions The weeding methods differ in in uence on soil bacterial community structure, soil properties, and plant growth, which are potentially useful as indices of biomass productivity to improve the growth of Chinese r seedlings.