2022
DOI: 10.1016/j.heliyon.2022.e11855
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A review of recent advances in plant-pathogen detection systems

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Cited by 34 publications
(22 citation statements)
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“…Lateral flow immunoassays (LFIAs) are yet another popular technique for the on-site detection of a wide variety of compounds including pathogens [ 176 ]. These are immunochromatographic assays comprised of metal nanoparticles, antibodies against the pathogen, and signal amplification probes functionalized in a test strip.…”
Section: Existing Optical Sensing Technologies To Monitor Plant-micro...mentioning
confidence: 99%
“…Lateral flow immunoassays (LFIAs) are yet another popular technique for the on-site detection of a wide variety of compounds including pathogens [ 176 ]. These are immunochromatographic assays comprised of metal nanoparticles, antibodies against the pathogen, and signal amplification probes functionalized in a test strip.…”
Section: Existing Optical Sensing Technologies To Monitor Plant-micro...mentioning
confidence: 99%
“…12,35 Among these, LAMP is particularly significant, as its set of primers enhances sensitivity, and intube colorimetric and fluorescent signals improve field applicability. 12,40 Isothermal amplification technologies do not require complex thermocyclers and can be integrated into simple hand-held devices, increasing their portability. 41 However, complex primer design, expensive reagents, and the need for instrumental product visualization introduce complexities in most of these methods compared to the CRISPR/Cas system.…”
Section: Current Molecular Diagnostic Tools For Plant Pathogensmentioning
confidence: 99%
“…For example, it is impossible to diagnose CLR on the stage of the masking of symptom using naked eye; and, it needs personal experiences using naked eye to identify the suspected symptoms to CLR in the coffee leaves, and sometimes, it is prone to misdiagnosis when symptoms and/or signs are not characteristic enough. Using molecular assays to detect plant pathogens is an effective way to accurately diagnose plant diseases at the early stages (Patel et al 2022;Salcedo et al 2021). Currently, several methods of molecular diagnostics have been developed for detection of H. vastatrix in coffee, including PCR using universal primers (White et al 1990) or specific primers (Wang et al 2017), and single-tube PCR (Wu et al 2022).…”
Section: Introductionmentioning
confidence: 99%
“…Currently, several methods of molecular diagnostics have been developed for detection of H. vastatrix in coffee, including PCR using universal primers (White et al 1990) or specific primers (Wang et al 2017), and single-tube PCR (Wu et al 2022). However, the disadvantages of these methods are time-consuming, laborious, high costs for reagents, specialized personnel and sophisticated equipment requirements; moreover, their sensitivity is still not enough to detect some low titer phytopathogens (Patel et al 2022;Salcedo et al 2021).…”
Section: Introductionmentioning
confidence: 99%