A review of RT-PCR technologies used in veterinary virology and disease control: Sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health

Hoffmann, Bernd; Reid, Scott M.; Mertens, Peter P. C.; Oura, C.A.L.; Rijn, Piet A. van; Slomka, Marek J.; Banks, Jill; Brown, Ian H.; Alexander, D. J.; King, Donald P.

doi:10.1016/j.vetmic.2009.04.034

Cited by 168 publications

(121 citation statements)

References 198 publications

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“…The advantages of TaqMan real-time PCR method over the conventional PCR assay are generally approved as real-time PCR is a more sensitive and specific method with a much shorter detection time. Detection and analysis of real-time PCR product is performed simultaneously during the amplification process by the software, whereas in the case of traditional PCR, the analysis of the results requires an additional step of agarose gel electrophoresis using factors such as ethidium bromide and UV light, which can be hazardous for human health (11,17). In the current research, TaqMan real-time PCR technique allowed detecting JSRV proviral DNA in 18.75% (15 out of 80) of blood samples which showed a high infection rate in apparently healthy sheep.…”

Section: Discussionmentioning

confidence: 99%

Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Bahari¹,

Ghannad²,

Dezfoulian³

et al. 2016

Journal of Veterinary Research

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Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

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Section: Discussionmentioning

confidence: 99%

Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Bahari¹,

Ghannad²,

Dezfoulian³

et al. 2016

Journal of Veterinary Research

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“…This technique allows to assess gene expression analysis, determination of viral load and detection of genetically modified organisms 68 . The primers are designed in such a way that a fluorescent signal is generated only when the primers are incorporated into an amplification product.…”

Section: Real Time Pcrmentioning

confidence: 99%

Bat Reservoirs for Rabies Virus and Epidemiology of Rabies in Colombia: a review

Jiménez

Lopéz

García

2017

Ces. Med. Vet. Zootec.

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Rabies is a fatal zoonotic disease caused by a neurotropic RNA virus part of the family Rhabdoviridae. The disease is characterized by encephalitic inflammation and is responsible for a number of annual fatal deaths of people and animals in the world. Chiropters such as insectivorous, frugivorous and hematophagous bats are the major transmitters, reservoirs, and vectors of the rabies virus. In Colombia, a total of thirty-five cases of human rabies occurred between 2000 -2014. Of the thirty-five cases twenty-two were transmitted by bats. The genetic variant V3 (hematophagous bats) were responsible for 24 human deaths, while the genetic variant V4, carried by insectivorous bats, caused three human deaths. The disease is mainly linked to infected cats that may have hunted infected bats and then transmitted the virus to humans. Diagnosis is usually made by detection of the virus from infected animals and techniques such as RT-PCR, which might be used to promote active surveillance in bat populations. Rabies is a preventable disease and vaccination of pets confers protective immunity, however, vaccination coverage of pets in Colombia is still limited. This review article collects epidemiological data of rabies virus genetic variants associated with chiropters species that have been reported to transmit the virus in the world and emphasizes on rabies cases reported in Colombia during the last decades. Keywords: Chiroptera, genetic variants, Rhabdoviridae, zoonotic diseases. ResumenLa rabia es una enfermedad zoonóticas mortal causada por un virus ARN neurotrópico de la familia Rhabdoviridae. La enfermedad se caracteriza por encefalitis y es responsable de múltiples muertes anuales de personas y animales. Quirópteros insectívoros, frugívoros y hematófagos son los principales transmisores, reservorios y vectores del virus. En Colombia, un total de 35 casos de rabia humana fueron reportados entre 2000-2014, veintidós de ellos fueron transmitidos por murciélagos. La variante genéti-ca V3 (murciélagos hematófagos) fue responsable de 24 muertes humanas, mientras que la variante genética V4 (murciélagos insectívoros) causó tres muertes humanas. La enfermedad es transmitida al humano principalmente

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“…CSFV has an unsegmented, plus-oriented, single-stranded RNA genome. Whereas the virus was traditionally detected by virus isolation on susceptible cell lines, RT-qPCR has become the method of choice for CSFV detection, based on its superior sensitivity and specificity (Hoffmann et al, 2009). Furthermore RT-qPCR still allows detecting CSFV-specific RNA in samples that do not contain any infectious virus anymore.…”

Section: Intmentioning

confidence: 99%

Comparison of various commercial products for phenol-guanidine-based classical swine fever virus RNA extraction

Hofmann¹,

Mader²

2014

Afr. J. Biotechnol.

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A review of RT-PCR technologies used in veterinary virology and disease control: Sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health

Cited by 168 publications

References 198 publications

Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Bat Reservoirs for Rabies Virus and Epidemiology of Rabies in Colombia: a review

Comparison of various commercial products for phenol-guanidine-based classical swine fever virus RNA extraction

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