Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Bahari, Aliasghar; Ghannad, Masoud Sabouri; Dezfoulian, Omid; Rezazadeh, F.; Sadeghi-Nasab, A.

doi:10.1515/jvetres-2016-0002

Cited by 6 publications

(8 citation statements)

References 25 publications

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“…The infection rate in the mediastinal lymph nodes was higher than that in lung tissues and blood; this finding was in agreement with that reported previously [6]. Furthermore, the findings of a study conducted in Iran [22] were consistent with these results, in that the detection rate in blood samples was higher than that associated with lung tissue samples. Interestingly, the gross findings of the infected lung were in line with the previous results [24]: The normal architecture of the lung was disturbed with enlargement of regional lymph nodes.…”

Section: Discussionsupporting

confidence: 92%

“…This result is in agreement with those of the previous studies in which the virus in the sheep was detected using PCR for the 1 st time in Ireland [19-21]. The finding is also supported by those of many studies in which OPA was diagnosed using PCR, such as the ones conducted in Iraq (5.55%) [10], India (8%) [12], Iran (13.75%) [22], and Northwest Iran (18%) [23]. Moreover, these findings are consistent with those of epizootic sheep diseases, reported in many countries except Australia, New Zealand, Falkland Islands, and Iceland [3].…”

Section: Discussionsupporting

confidence: 92%

“…The histopathological analyses were useful in supporting the results of PCR in this study. The analyses revealed tumor nodules infiltrating the alveoli with excessive infiltration of macrophages around a neoplastic focus in lung tissue as well as hypertrophic changes in lymph nodes; these findings were in agreement with previously reported ones [22,25,26]. Further, in the lymph nodes, neoplastic cells occupied the subcapsular and paratrabecular sinuses or showed locally extensive growth, replacing the normal cortex [27,28].…”

Section: Discussionsupporting

confidence: 90%

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Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

et al. 2019

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Aim: This study aimed to conduct a clinical-histopathological and molecular evaluation of ovine pulmonary adenocarcinoma (OPA) in Awassi sheep in various regions of Al-Qadisiyah Province, Iraq. Materials and Methods: A total of 150 sheep were clinically evaluated, and the wheelbarrow test was performed. 100 samples (35 blood, 25 lung tissue, 20 lymph node, and 20 lung fluid samples) were randomly selected from living and slaughtered sheep. All samples were subjected to polymerase chain reaction (PCR). Histopathological examinations were performed for four lung tissue and two lymph node samples. Results: A diagnosis of OPA was made based on the results of the clinical examination and the clinical signs shown by the animals, such as dyspnea, polypnea, coughing, mucous nasal discharge, moist rales on auscultation of the affected lungs, and emaciation. Interestingly, the animals tested positive for the wheelbarrow test, with frothy nares accompanied by profuse and clear lung fluid. Histopathological examination showed various lesions such as glandular transformation in the lung tissues and emphysema. Moreover, lymph nodes showed marked follicular atrophy and necrosis-associated lymphocyte infiltration in the affected tissues. PCR revealed that 25% of the samples including eight (22.8%) blood, five (20%) lung tissue, five (25%) lymph node, and seven (35%) lung fluid samples were positive for Jaagsiekte sheep retrovirus; this result was highly significant. Conclusion: The results of our study indicated that in Iraq, OPA diagnosis should be based on pathological findings and results of advanced procedures such as PCR.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

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Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

et al. 2019

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“…These recent studies were in agreement with (17)(18)(19)). In the current study, samples were collected from affected sheep based on the typical clinical features of OPA.…”

Section: Discussionsupporting

confidence: 90%

Phylogenetic analysis of Jaagsiekte sheep retrovirus (JSRV) in Iraqi Awassi sheep

Al-Husseiny¹,

Jassim²,

Mansour³

et al. 2020

IJVS

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Jaagsiekte sheep retrovirus (JSRV) infection in sheep results in contagious ovine pulmonary adenocarcinoma (OPA). The aim of this study was to detect the presence of JSRV in Iraqi Awassi sheep of Al-Qadisiyah Province using reverse transcription-PCR (RT-PCR). Lung secretion samples from 50 adult sheep (1.5 to 2-year-old) during 2016-2018 that were suffering from chronic respiratory distress with suspected clinical OPA were collected. RT-PCR assay for detection of JSRV was conducted using primers specific for its 382 bp envelope protein gene. The results showed that 22 of the 50 (44%) lung secretion samples were positive for JSRV. Phylogenetic tree analysis revealed the JSRV sequence detected in the local Iraqi sheep population was closely related to NCBI BLAST locus isolates KT279066.1 and KT279065.1. The study found that the JSRV disease is prevalent in the Iraqi Awassi sheep flocks and that the local JSRV isolate is genetically divergent from most other global isolates.

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“…For example, the use of PCR on blood from live sheep tends to yield higher JSRV prevalence estimates than do abattoir surveys using gross examination and histopathology. Lewis et al (2011) used PCR on blood samples which resulted in a JSRV prevalence of 3.6% in sheep in Scotland [ 9 ], and even higher prevalence estimates of 18.75% and 18% were obtained in studies based in the central-west of Iran and northwest Iran, respectively, using PCR on blood samples [ 32 , 33 ]. As PCR detects JSRV in circulating leukocytes, this technique can identify infection in latently and subclinically infected animals.…”

Section: Discussionmentioning

confidence: 99%

First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter

et al. 2017

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BackgroundOvine pulmonary adenocarcinoma (OPA), caused by Jaagsiekte sheep retrovirus (JSRV), is characterised by the development of invariably fatal lung tumours primarily in adult sheep. High infection rates and disease prevalence can develop during initial infection of flocks, leading to on-farm economic losses and animal welfare issues in sheep with advanced disease. The disease has been reported in Ireland and is notifiable, but the presence of JSRV has never been confirmed using molecular methods in this country. Additionally, due to the difficulties in ante-mortem diagnosis (especially of latently-infected animals, or those in the very early stages of disease), accurate information regarding national prevalence and distribution is unavailable. This study aimed to confirm the presence of JSRV in Ireland and to obtain estimates regarding prevalence and distribution by means of an abattoir survey utilising gross examination, histopathology, JSRV-specific reverse transcriptase polymerase chain reaction (RT-PCR) and SU protein specific immunohistochemistry (IHC) to examine the lungs of adult sheep.ResultsLungs from 1911 adult sheep were examined macroscopically in the abattoir and 369 were removed for further testing due to the presence of gross lesions of any kind. All 369 were subject to histopathology and RT-PCR, and 46 to IHC. Thirty-one lungs (31/1911, 1.6%) were positive for JSRV by RT-PCR and/or IHC but only ten cases of OPA were confirmed (10/1911, 0.5%) Four lung tumours not associated with JSRV were also identified. JSRV-positive sheep tended to cluster within the same flocks, and JSRV-positive sheep were identified in the counties of Donegal, Kerry, Kilkenny, Offaly, Tipperary, Waterford and Wicklow.ConclusionsThe presence of JSRV has been confirmed in the Republic of Ireland for the first time using molecular methods (PCR) and IHC. In addition, an estimate of OPA prevalence in sheep at slaughter and information regarding distribution of JSRV infection has been obtained. The prevalence estimate appears similar to that of the United Kingdom (UK). Results also indicate that the virus has a diverse geographical distribution throughout Ireland. These data highlights the need for further research to establish national control and monitoring strategies.Electronic supplementary materialThe online version of this article (10.1186/s13620-017-0111-z) contains supplementary material, which is available to authorized users.

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Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Cited by 6 publications

References 25 publications

Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

Phylogenetic analysis of Jaagsiekte sheep retrovirus (JSRV) in Iraqi Awassi sheep

First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter

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