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Jaagsiekte sheep retrovirus (JSRV) infection in sheep results in contagious ovine pulmonary adenocarcinoma (OPA). The aim of this study was to detect the presence of JSRV in Iraqi Awassi sheep of Al-Qadisiyah Province using reverse transcription-PCR (RT-PCR). Lung secretion samples from 50 adult sheep (1.5 to 2-year-old) during 2016-2018 that were suffering from chronic respiratory distress with suspected clinical OPA were collected. RT-PCR assay for detection of JSRV was conducted using primers specific for its 382 bp envelope protein gene. The results showed that 22 of the 50 (44%) lung secretion samples were positive for JSRV. Phylogenetic tree analysis revealed the JSRV sequence detected in the local Iraqi sheep population was closely related to NCBI BLAST locus isolates KT279066.1 and KT279065.1. The study found that the JSRV disease is prevalent in the Iraqi Awassi sheep flocks and that the local JSRV isolate is genetically divergent from most other global isolates.

Aim: This study aimed to conduct a clinical-histopathological and molecular evaluation of ovine pulmonary adenocarcinoma (OPA) in Awassi sheep in various regions of Al-Qadisiyah Province, Iraq. Materials and Methods: A total of 150 sheep were clinically evaluated, and the wheelbarrow test was performed. 100 samples (35 blood, 25 lung tissue, 20 lymph node, and 20 lung fluid samples) were randomly selected from living and slaughtered sheep. All samples were subjected to polymerase chain reaction (PCR). Histopathological examinations were performed for four lung tissue and two lymph node samples. Results: A diagnosis of OPA was made based on the results of the clinical examination and the clinical signs shown by the animals, such as dyspnea, polypnea, coughing, mucous nasal discharge, moist rales on auscultation of the affected lungs, and emaciation. Interestingly, the animals tested positive for the wheelbarrow test, with frothy nares accompanied by profuse and clear lung fluid. Histopathological examination showed various lesions such as glandular transformation in the lung tissues and emphysema. Moreover, lymph nodes showed marked follicular atrophy and necrosis-associated lymphocyte infiltration in the affected tissues. PCR revealed that 25% of the samples including eight (22.8%) blood, five (20%) lung tissue, five (25%) lymph node, and seven (35%) lung fluid samples were positive for Jaagsiekte sheep retrovirus; this result was highly significant. Conclusion: The results of our study indicated that in Iraq, OPA diagnosis should be based on pathological findings and results of advanced procedures such as PCR.

Objective: 1-Isolation and diagnosis of mites species in sheep. 2-To study the pathological changes in the skin. 3-To know the epidemiological distribution of Scabies in human. Materials and Methods: Scrabing was collected from infected area of skin of sheep and human. These specimens were treated with 10% KOH to remove scales and hairs, and then fixed on slides for diagnosis. Biopsies were taken from infected skin for pathological study. Results: Three species of mites were diagnosed on sheep, Sarcoptes scabiei, Psoroptes ovis and Chorioptes sarcoptes, with percents of infection 34.95%, 58.27%, 4.71% respectively. Gross pathological changes observed were, toughness, dryness, fissuring of the skin with white scales, while microscopical pathological changes revealed, hyperkeratosis, acanthosis, infiltration of chronic inflammatory cells (eosinophils, macrophages and lymphocytes) and oedema in the dermis. Age group of 11-30 years of age were highly affected (28.2%). Housewives were the highest percent among other occupation (29.1%). Conclusions: Scabies is a big public health problem and should be looked after among sheep as well as human beings.

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