First molecular diagnosis of ovine pulmonary adenocarcinoma in Awassi sheep in Iraq

Jassim, Asaad; Al-Husseiny, Saad Hashim; Mansour, Kalefa Ali; Kshash, Qassim Haleem

doi:10.29079/vol16iss1art46

Cited by 4 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is in agreement with those of the previous studies in which the virus in the sheep was detected using PCR for the 1 st time in Ireland [19-21]. The finding is also supported by those of many studies in which OPA was diagnosed using PCR, such as the ones conducted in Iraq (5.55%) [10], India (8%) [12], Iran (13.75%) [22], and Northwest Iran (18%) [23]. Moreover, these findings are consistent with those of epizootic sheep diseases, reported in many countries except Australia, New Zealand, Falkland Islands, and Iceland [3].…”

Section: Discussionsupporting

confidence: 92%

“…Moreover, these findings are consistent with those of epizootic sheep diseases, reported in many countries except Australia, New Zealand, Falkland Islands, and Iceland [3]. It was identified JSRV in all samples by PCR; these findings were similar to those reported previously [10,20], in terms of chronicity, carcinogenic character, and long duration of the disease, which allow the infection to spread to most tissues. However, it was found a higher infection rate in the lung fluid samples than in the other samples, consistent with the previous results [14].…”

Section: Discussionsupporting

confidence: 91%

“…To the best of our knowledge, thus far, only two studies on JSRV-induced OPA have been performed in Iraq: First, in Mosul province using histopathological analyses [9] and the second in Al-Qadisiyah Province using polymerase chain reaction (PCR) [10]. Thus, this study aimed to confirm the presence of this disease in sheep in Al-Qadisiyah Province using PCR in addition to clinical and histopathological evaluations.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

et al. 2019

Self Cite

View full text Add to dashboard Cite

Aim: This study aimed to conduct a clinical-histopathological and molecular evaluation of ovine pulmonary adenocarcinoma (OPA) in Awassi sheep in various regions of Al-Qadisiyah Province, Iraq. Materials and Methods: A total of 150 sheep were clinically evaluated, and the wheelbarrow test was performed. 100 samples (35 blood, 25 lung tissue, 20 lymph node, and 20 lung fluid samples) were randomly selected from living and slaughtered sheep. All samples were subjected to polymerase chain reaction (PCR). Histopathological examinations were performed for four lung tissue and two lymph node samples. Results: A diagnosis of OPA was made based on the results of the clinical examination and the clinical signs shown by the animals, such as dyspnea, polypnea, coughing, mucous nasal discharge, moist rales on auscultation of the affected lungs, and emaciation. Interestingly, the animals tested positive for the wheelbarrow test, with frothy nares accompanied by profuse and clear lung fluid. Histopathological examination showed various lesions such as glandular transformation in the lung tissues and emphysema. Moreover, lymph nodes showed marked follicular atrophy and necrosis-associated lymphocyte infiltration in the affected tissues. PCR revealed that 25% of the samples including eight (22.8%) blood, five (20%) lung tissue, five (25%) lymph node, and seven (35%) lung fluid samples were positive for Jaagsiekte sheep retrovirus; this result was highly significant. Conclusion: The results of our study indicated that in Iraq, OPA diagnosis should be based on pathological findings and results of advanced procedures such as PCR.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The prevalence of ovine pulmonary adenocarcinomas was found to be at 15.4% in the current study; this percentage was higher than that recorded by Al-Ajeli et al (6), who recorded this type of tumor in 11.2%, this may be related to the population of animals that included in the current study were they investigate this lesion during three months in one flock of sheep, another study conducted by Jassim et al (11) were they recorded 10.1% of this tumor in sheep in Al-Qadisiyah city. Ovine pulmonary adenocarcinomas have a pathognomic lesion characterized by a neoplastic cuboidal and columnar cell that project as papillary of acinar form inside alveoli and bronchioles surrounded by dense fibrous connective tissue (5).…”

Section: Discussioncontrasting

confidence: 65%

Histopathology and Immunohistochemistry of tumors in animals attending veterinary teaching hospital

Al-Mahmood¹,

Khalil²,

Edreesi³

2022

IJVS

View full text Add to dashboard Cite

We aim in the current study to investigate the pathology and incidence of tumors that are excised surgically in Veterinary Teaching Hospital. This study collected the tissue samples from animals from 1 October 2020 until 1 April 2021. These samples were collected from animals that undergo surgical procedures to remove neoplastic growths in their body. After tissue grossing, samples were collected from tumor mass, then fixed in neutral buffered 10% formalin for 72 hours, then processed to embedded in paraffin wax. A routine Harris's haematoxylin and eosin stain were also used, histochemical stains such as Masson's trichrome and PAS techniques were used as needed. In exceptional cases, IHC protocol was used for diagnostic steps to some types of tumors included in the current study. The antibodies that were used are Vimentin for canine osteosarcoma, P53 for squamous cell carcinoma, Mdm2 for ovine pulmonary adenocarcinoma, and CD10 for neoplastic lymphocytes. The result of the current study showed that the prevalence of tumor recorded was Marek's diseases 22.8%, squamous cell carcinoma 22.8%, ovine pulmonary adenocarcinoma 15.4%, third eyelid adenoma 15.4%, canine osteosarcomas 7.4%, venereal transmitting tumors 7.4%, and feline mammary gland tumor 7.4%. In conclusion, the incidence of tumor in animals that attending Veterinary Teaching Hospital belong to the College of Veterinary Medicine, the University of Mosul was in general high in comparison to other studies in Iraq and other countries, this high incidence should pay attention to the causes of these cancerous conditions and their relation to environmental aetiology.

show abstract

“…The diseases resulting from an infection of a virus belonging to a beta retrovirus called Jaagsiekte sheep retrovirus (JSRV) [1]. OPA was first recorded in South Africa in the 19th century where it was called Jaagsiekte which is derived from the Afrikaans for (chasing sicknesses) and disease has been noticed in wide types of breeds of sheep and in different countries over the world [2][3][4][5]. The characteristic of tumor cells in OPA thought to be originated from type two of alveolar epithelial cells or Clara cells, which is recorded as secretary cells and lining the epithelium of alveoli of the lung therefore, the disease is characterized by excessive secretion fluids by the tumor cells and these fluids are accumulated in the lung, which be sources of infectious virus and causes important economic and animal welfare problems [6].…”

Section: Introductionmentioning

confidence: 99%

Ovine and Caprine Pulmonary Adenomatosis, At Duhok Abattoir, Iraq, First Prevalence and Pathological Study

Abdullah

2023

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

T HE current study aimed to investigate the prevalence and pathological observation of ovine pulmonary adenocarcinoma (OPA) in sheep and goats at Duhok abattoir of Iraq. A total of 4061 lung samples from small ruminants (3667 sheep and 394 goats) were selected randomly from slaughtered sheep and goats. All samples were examined macroscopically is involving color, consistency, texture, adhesion, pattern, the number of the lesion, distribution, the nature of exudates and lobe of the lung are involved. Histopathological examinations were performed from 55 affected lungs The results showed that the prevalence of disease in the Duhok abattoir was 1.35% based on macroscopical features while results found 45.45% percentages of affected lung with OPA according to histopathological features, the gross lesions of OPA in sheep and goatsfound that the upper respiratory part was filled with excessive frothy material fluid and the lungs were heavy, enlargement, and edematous appearance. While the important histopathological observations of the affected parts was varied from small discrete nodules that appears white in color and are involves both lungs, measuring around 0.5-2 cm of the diaphragmatic and other lobes were recorded, and classified as classical and atypical form. Histologically results showed the presence of multiple proliferations foci of neoplastic cells in the epithelial cell of alveoli and bronchi, these proliferations appear either papillary or as the impression of solid growths appearance or acinar shape. Inconclusion our study gives important data about the prevalence and pathological changes of OPA in sheep and goats at the Duhok abattoir of Iraq.

show abstract

First molecular diagnosis of ovine pulmonary adenocarcinoma in Awassi sheep in Iraq

Cited by 4 publications

References 0 publications

Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

Clinical-histopathological and molecular study of ovine pulmonary adenocarcinoma in Awassi sheep in Al-Qadisiyah Province, Iraq

Histopathology and Immunohistochemistry of tumors in animals attending veterinary teaching hospital

Ovine and Caprine Pulmonary Adenomatosis, At Duhok Abattoir, Iraq, First Prevalence and Pathological Study

Contact Info

Product

Resources

About