The capability of temperature variation is essential for cooling industrial operations like transportation such as car and heavy vehicle radiators, electronics devices, petroleum industrial systems, etc. Different methods and fluids are used in the cooling process in industrial systems. The basic fluids are based on temperature, thermal stability, and the effectiveness of heat transmission. Thermal characteristics improve when nanoparticles are added to the basic fluid. Using Al2O3 nanofluid the heat transfer and variation in the temperature at the entrance side and outlet side of the microchannel pipe were studied. Through ANSYS Fluent, a well-defined method for utilizing Al2O3 nanofluids to investigate the impact of various performance optimization factors of nanofluids was performed. The temperature of the nanofluids at the inlet and outlet is found 300 K and 313.7 K, respectively during the simulation. The pressure drops from the inlet side to the outlet side as well a result that raising the temperature, heat coefficient, thermal conductivity, and viscosity of the base fluids when Al2O3 nanoparticles are added.