A Review of the Impact of Microfluidics Technology on Sperm Selection Technique

Abstract

Sperm sorting procedures depend on centrifugation processes. These processes produce oxidative stress and cell damage that are undesirable for in-vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes because they affect fertilization and implantation chances. The microfluidic sperm selection technique has shown promise in this area. It can create a platform for isolating and manipulating goodquality sperm cells using diverse triggers such as mechanical factors, chemical agents, and temperature gradients. Furthermore, microfluidic platforms can direct sperm cells for IVF or sperm sorting by utilizing an approach that is passive or active. In this review, we explain the use of microfluidics technologies for sorting and arranging sperm cells for different purposes. We also discuss the use of microfluidics technology in selecting and assessing sperm parameters and how it affects male infertility.

Separation of sperm based on rheotaxis mechanism using microfluidic device

“…Conversely, the application of micro uidic techniques can be instrumental in minimizing the occurrence of sperm with abnormal morphology, such as those with head or tail defects. By employing micro uidic technology, the presence of such anomalies can be effectively reduced, thereby enhancing the overall quality and functionality of the sperm (40,41).…”

Alginate Silver Nanoparticles and Their Effect on Sperm Parameters of the Domestic Rabbit

“…Another area of research focuses on the development of a diagnostic method to evaluate changes in the morphology and morphometry of preserved sperm and to clarify the difficulties associated with cryopreservation [18,70]. The semen parameters of all animal species, including rabbits, in addition to the semen collection technique itself, are affected by many factors, including those related to the breeding environment (such as the cage housing system), nutrition, health status, time of year, season or age of the males [71].…”

Comparison of the Effectiveness of Density Gradient Centrifugation and Microchip Sperm Preparation Methods on Pregnancy Success in Intrauterine Insemination

“…In order to eliminate these effects, microchip method has been developed in sperm preparation in recent years. In this method, healthy sperm can pass through a filter system without the need for centrifugation and sperm with smooth morphology, high motility and no DNA damage was collected (17,18).…”