A review of the use of Streptozotocin (STZ) in the  induction of diabetes in rats and subsequent ocular  tissue changes

Sithole, H. L.

doi:10.4102/aveh.v68i2.156

Cited by 8 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After treatment with low dose of STZ survival and regeneration of pancreatic β-cells is possible in type I diabetes [ 41 ]. Streptozotocin (2-deoxy-2-(N-methyl-N-nitrsourea) -1-D-glucopyranose) is a nitrosourea compound obtained from the soil microbe Streptomyces achromogenes [ 42 ] , which is the potent alkylating agent that exerts its effect on the pancreatic β-cells via glucose transporter-GLUT2, causing induction of methylation of DNA and results in damage of DNA. Damaged DNA, activate the poly (ADP-Ribose) polymerase, leading to NAD + depletion in pancreatic β-cells [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Paederia foetida Linn. leaf extract: an antihyperlipidemic, antihyperglycaemic and antioxidant activity

Kumar

Anwar²,

Ahmed

et al. 2014

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundThe primary objective of the present investigation is to evaluate the antidiabetic, antihyperlidemic and antioxidant activity of the methanolic extract of the Paederia foetida Linn. (PF) leaf extract in the streptozotocin induced diabetic rats.MethodsSingle intraperitoneal injection (IP) of streptozotocin (60 mg/kg body weight) was used for induction of diabetes is swiss albino (wistar strain) rats. The induction of diabetes was confirmed after 3 days as noticing the increase in blood sugar level of tested rats. PF at a once a daily dose of 100 mg/kg, 250 mg/kg, 500 mg/kg, p.o. along with glibenclamide 10 mg/kg, p.o. was also given for 28 days. On the 28th day rats from all the groups fasted overnight fasted and the blood was collected from the puncturing the retro orbit of the eye under mild anesthetic condition. There collected blood sample was used to determine the antihyperlipidemic, hypoglycemic and antioxidant parameters.ResultsThe oral acute toxicity studies did not show any toxic effect till the dose at 2000 mg/kg. While oral glucose tolerance test showed better glucose tolerance in tested rats. The statistical data indicated that the different dose of the PF significantly increased the body weight, hexokinase, plasma insulin, high density lipoprotein cholesterol, superoxide dismutase, catalase and glutathione peroxides. It also decreases the level of fasting blood glucose, total cholesterol, triglycerides, low density lipoprotein cholesterol, very low density lipoprotein cholesterol, malonaldehyde, glucose-6-phosphate, fructose-1-6-biphosphate and glycated hemoglobin in STZ induced diabetic rats. The histopathology of STZ induce diabetic rats, as expected the test dose of PF extract considerably modulates the pathological condition of various vital organ viz. heart, kidney, liver, pancreas as shown in the histopathology examinations.ConclusionsOur investigation has clearly indicated that the leaf extract of Paederia foetida Linn. showed remarkable antihyperglycemic activity due to its possible systematic effect involving in the pancreatic and extra pancreatic mechanism. Forever, the antihyperlipidemic activity was exerted possible by lowering the higher level of lipid profile and decreasing the intercalated disc space in the heart. The antioxidant activity of extract was due to inhibition of lipid peroxidation and increasing the SOD, GPx and CAT. It was corroborate that the extract shown the Paederia foetida Linn leaves potential to be act as antidiabetic, antihyperlipidemic and antioxidant properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Paederia foetida Linn. leaf extract: an antihyperlipidemic, antihyperglycaemic and antioxidant activity

Kumar

Anwar²,

Ahmed

et al. 2014

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…STZ is a nitrosourea compound, widely used cytotoxic agent and obtained from the soil microbe Streptomyces achromogenes, which effect on pancreatic β-cells induced the diabetes [ 29 ]. STZ affects pancreatic β-cells, secreted on endogenous insulin which arrest stating the secretion of insulin; as a result the level of insulin increase in blood, due to increasing levels of insulin it increases the level of blood glucose [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Umbelliferone β-D-galactopyranoside from Aegle marmelos (L.) corr. an ethnomedicinal plant with antidiabetic, antihyperlipidemic and antioxidative activity

Kumar

Ahmed

Verma

et al. 2013

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundAegle marmelos (L.) Corr. (Rutaceae), commonly known as bael, is used to treat fevers, abdomen pain, palpitation of the heart, urinary troubles, melancholia, anorexia, dyspepsia, diabetes and diarrhea in Indian traditional systems of medicine. The object of the present study was to evaluate the antidiabetic, antihyperlipidemic and antioxidant oxidative stress of umbelliferone β-D-galactopyranoside (UFG) from stem bark of Aegle marmelos Correa. in STZ (streptozotocin) induced diabetic rat.MethodsDiabetes was induced in rat by single intraperitoneal injection of STZ (60 mg/kg). The rat was divided into the following groups; I – normal control, II – diabetic control, III – UFG (10 mg/kg), IV – UFG (20 mg/kg), V – UFG (40 mg/kg), VI – Glibenclamide (10 mg/kg, p.o., once a daily dose). Diabetes was measured by change the level blood glucose, plasma insulin and the oxidative stress were assessed in the liver by estimation of the level of antioxidant markers i.e. superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and Malondialdehyde (MDA) and antihyperlipidemic effect was measured by estimation of total cholesterol, triglycerides, LDL (low density lipoprotein) cholesterol, HDL (high density lipoprotein) cholesterol, VLDL (very low density lipoprotein) cholesterol. However in a study, the increased body weight was observed and utilization of glucose was in the oral glucose tolerance test.ResultDaily oral administration of different dose of UFG for 28 days showed significantly (P < 0.001) decreased in fasting blood glucose level and improve plasma insulin level as compared to the diabetic control group. Also it significantly (P < 0.001) decreased the level of glycated hemoglobin, glucose-6-phosphatase, fructose-1-6-biphosphate and increased the level of hexokinase. UFG treatment decreased liver MDA and increased the level of SOD, GPx and CAT. UFG treatment of lipids it’s increased the level of cholesterol, triglycerides, VLDL, LDL cholesterol and decreased the level of HDL cholesterol. Histologically, inflammatory cell in blood vessels, intercalated disc, fat degeneration and focal necrosis observed in diabetic rat organ but was less obvious in UFG treated groups. The mechanism of action of UFG may be due to the increased level of pancreatic insulin secretion and effect on the antioxidant marker.ConclusionUFG posses an antidiabetic, antioxidant and antihyperlipidemic effect on the STZ induced diabetic rat. Hence it could be the better choice to cure the diabetes.

show abstract

“…Recently, there are increasing interest for the development and utilization of antidiabetic plants, owing to their renewable resource and possible beneficial effects besides other economic reasons (Zhao et al 2007). According to Sithole (2009), streptozotocin (STZ)-induced diabetes can be treated by plant extracts which control the blood sugar level as well as improving the lipid profile and ocular complications such as retinopathy. Among these plants, aloe vera has been fingered on that is from the family of Liliaceae and has about 360 species.…”

Section: Discussionmentioning

confidence: 99%

Histomorphometric alterations in aloe vera gel extract treatment in the diabetic rat’s retina

Gholami

Saberi

2014

Comp Clin Pathol

View full text Add to dashboard Cite

The retina is a part of the central nervous system in terms of histological and embryological aspect. It is a thin layer of neural tissue lining the inner eye. This study assessed the histomorphometric changes in the thicknesses of the retina and its layers using light microscope (LM) and retinal photoreceptor cells using transmission electron microscope (TEM) as a consequence of diabetes and effect of aloe vera gel extract in male rats. Thirty Sprague-Dawley matured male rats (175±25 g) in two age groups (12 and 16 weeks) were divided into three main groups as control, diabetic, and diabetic receiving 400 mg/kg aloe vera extract. There were two subgroups in each group. For inducing diabetes, a single dose of streptozotocin (STZ) (50 mg/kg) was injected through IP route. Their blood sugar was measured by a glucometer before STZ administration and 24 h thereafter. At the end, animals were anesthetized with sodium thiopental (40 mg/kg) via IP injection. The retina was collected for tissue processing following dissection of eyes, fixed in 4 % glutaraldehyde, postfixed in osmium tetroxide 1 %, dehydrated, and then embedded in TAAB resin. Semithin sections (1 μm) were stained with toluidine blue stain and viewed under light microscope. Ten slides were prepared from each animal. Ultrathin sections were prepared, stained, and viewed under TEM. The blood glucose levels and body weight in treated rats were reduced in comparison to diabetic groups. The neural retina thickness and its layers were also different. In the aloe vera-treated group, the thickness of the retina and its layers retained their normal histological structures. In diabetic groups, the vesicular mitochondria and degenerated cristae and dense nucleus were observed in photoreceptor cells. After treatment with aloe vera extract, cristae gained partially their arrangement and chromatin spread. It is concluded that the degenerated retina and the thickness of different layers of diabetic retina could be affected with long-term treatment of aloe vera gel extract.

show abstract

A review of the use of Streptozotocin (STZ) in the induction of diabetes in rats and subsequent ocular tissue changes

Cited by 8 publications

References 0 publications

Paederia foetida Linn. leaf extract: an antihyperlipidemic, antihyperglycaemic and antioxidant activity

Paederia foetida Linn. leaf extract: an antihyperlipidemic, antihyperglycaemic and antioxidant activity

Umbelliferone β-D-galactopyranoside from Aegle marmelos (L.) corr. an ethnomedicinal plant with antidiabetic, antihyperlipidemic and antioxidative activity

Histomorphometric alterations in aloe vera gel extract treatment in the diabetic rat’s retina

Contact Info

Product

Resources

About