A review of transcriptome studies combined with data mining reveals novel potential markers of malignant pleural mesothelioma

Melaiu, Ombretta; Cristaudo, Alfonso; Melissari, Erika; Russo, M.; Bonotti, Alessandra; Bruno, Rossella; Foddis, Rudy; Gemignani, Federica; Pellegrini, Silvia; Landi, Stefano

doi:10.1016/j.mrrev.2011.12.003

Cited by 46 publications

(47 citation statements)

References 69 publications

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“…In recent years, a number of aberrantly expressed genes were suggested, but with a poor consistency among studies. In 2012, in order to find a consensus among nine independent transcriptome studies carried out between 2000 and 2010 (1)(2)(3)(4)(5)(6)(7)(8)(9), it has been performed a review and a data mining (10) and it has been identified a group of 96 overexpressed genes in MPM compared to non-malignant tissues and cell lines. This group of genes have been further evaluated in 2015 through a gene expression study performed on an independent cohort of mesothelioma tissues compared to non-malignant tissues: the overexpression of 51 genes ( Table 1) have been confirmed (11).…”

Section: Overexpressed Genes Identified In Malignant Pleural Mesothelmentioning

confidence: 99%

“…Normal mesothelial cells show low levels of MSLN and it is undetectable in most normal tissues. On the contrary, mesothelin is overexpressed in several human cancers, including MPM (10,11). In the last years mesothelin has become an active topic of investigation in MPM.…”

Section: Clinical Implications Of Overexpressed Genes In Mpmmentioning

confidence: 99%

“…In the multicenter phase 2 randomized clinical trial, conducted between March 2011 and May 2013, has been observed that arginine deprivation with ADI-PEG20 in ASS1-negative mesothelioma patients improved the progression free survival (116). About 50% of mesothelioma appeared to be ASS1-negative (117) but ASS1 has been found overexpressed in a significant number of mesothelioma patients (10,11,82). ASS1 overexpression is linked with chloroquine and cisplatin resistance in mesothelioma: high ASS1 expression in mesothelioma cells decreased their sensitivity to chloroquine toxicity (118) and ASS1 silencing in MPM spheroids increased the apoptotic response to cisplatin plus pemetrexed (82), rather suggesting an important contribution to the onset of chemo-resistance in MPM.…”

Section: Clinical Implications Of Overexpressed Genes In Mpmmentioning

confidence: 99%

“…CDC2 codifies for a cyclin dependent kinase 1 (CDK1) and it has been found overexpressed in mesothelioma in three independent transcriptome studies (10). CDK1 knockdown could provide a novel therapeutic approach to arrest cell-cycle progression in MPM cells: in 2009 it has been observed that CDC2 silencing increased the apoptotic fraction of MPM cells (85) and in 2014, the RNA-interference screening performed on MPM cell-lines confirmed its role in cell-cycle and in the apoptosis (86).…”

Section: Clinical Implications Of Overexpressed Genes In Mpmmentioning

confidence: 99%

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Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

2018

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Malignant pleural mesothelioma (MPM) is a very aggressive cancer poorly responsive to current therapies. MPM patients have a very poor prognosis with a median survival of less than one year from the onset of symptoms. The biomarkers proposed so far do not lead to a sufficiently early diagnosis for a radical treatment of the disease. Thus, the finding of novel diagnostic and prognostic biomarkers and therapeutic targets is needed. Gene overexpression has been frequently associated with a malignant phenotype in several cancer types; therefore the identification of overexpressed genes may lead to the detection of novel prognostic or diagnostic marker and to the development of novel therapeutic approaches, based on their inhibition. In the last years, several overexpressed genes have been identified in MPM through gene expression profiling techniques: among them it has been found a group of 51 genes that resulted overexpressed in more than one independent study, revealing their consistency among studies. This article reviews the clinical implications of confirmed overexpressed genes in MPM described so far in literature.

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Section: Overexpressed Genes Identified In Malignant Pleural Mesothelmentioning

confidence: 99%

Section: Clinical Implications Of Overexpressed Genes In Mpmmentioning

confidence: 99%

Section: Clinical Implications Of Overexpressed Genes In Mpmmentioning

confidence: 99%

Section: Clinical Implications Of Overexpressed Genes In Mpmmentioning

confidence: 99%

See 2 more Smart Citations

Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

2018

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“…Very recently, we carried out a study, which through a preliminary paired genomic and proteomic approach (63,64) allowed us to test in the serum by the use of specific ELISA, a set of putative blood markers for the diagnosis of MPM. Each of these markers were singularly tested for their diagnostic performance as well as in combination with one or more of the other, using a logistic regression method (65).…”

Section: Multiple Biomarkers Panelsmentioning

confidence: 99%

Biomarkers in the prevention and follow-up of workers exposed to asbestos

Foddis¹,

Bonotti²,

Landi³

et al. 2018

J. Thorac. Dis.

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Although in most developed countries the use of asbestos is banned, there is still a consistent portion of the world where asbestos extraction, trading and manufacturing of asbestos-made products is largely diffuse. Worldwide, hundreds of millions of people are at risk of developing an asbestos caused disease because of occupational, environmental or domestic exposure. The WHO estimates that asbestos is responsible for more than 100,000 deaths yearly. This scenario has prompted the research on biomarkers potentially useful for early diagnosis, prognosis and preventive programs on exposed population as well. Here we reviewed the up-to-date literature on this field of research highlighting that along with mesothelin and osteopontin (OPN), some more recently investigated molecules, such as high mobility group box 1 (HMGB1) protein, fibulin-3 and some miRNAs showed very promising. Most of the carried-out studies showed an interesting diagnostic and prognostic performance of some biomarkers, but since they usually lack adequate either specificity or sensitivity, their use in screening or in preventive programs is still not recommended on a routine basis. However, this review suggests the need for more reliable experimental design involving larger population and preferring longitudinal screening of asbestos exposed individuals rather than a single baseline assessment investigation. In addition, given their better diagnostic accuracy, the use of panels including several biomarkers is highly recommended.

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Profiling of the silica-induced molecular events in lung epithelial cells using the RNA-Seq approach

et al. 2017

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Silicosis is a prolonged, irreversible and incurable occupational disease, and there is a significant number of newly diagnosed cases every year in Hong Kong. Due to the long latency of the disease, the diagnosis can be missed until detailed clinical examination at a later stage. For a better control of this deadly disease, detailing the pro-inflammatory and fibrotic events in the macrophage would be instrumental in understanding the pathogenesis of the disease and essential for the significant biomarkers discovery. In this in vitro study, human cell line model A549 lung epithelial cells were used. The immediate molecular events underneath the activation of quartz silica polymorphs were followed in a time course of 0, 0.5, 2, 8, 16 and 24 h. The transcriptome library was prepared and subjected to RNA-Seq analysis. Data analysis was performed by pathway analysis tools and verified by real-time PCR. The results showed that triggered genes were mainly found in the immune response and inflammatory pathways. An interesting finding was the association of the DNA-binding protein inhibitor (ID) family in the silica exposure to lung cells. The linkage of ID1, ID2 and ID3 to cancer may rationalize themselves to be the markers indicating an early response of silicosis. However, further studies are required to consolidate the roles of these genes in silicosis. Copyright © 2017 John Wiley & Sons, Ltd.

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A review of transcriptome studies combined with data mining reveals novel potential markers of malignant pleural mesothelioma

Cited by 46 publications

References 69 publications

Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

Biomarkers in the prevention and follow-up of workers exposed to asbestos

Profiling of the silica-induced molecular events in lung epithelial cells using the RNA-Seq approach

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