A Review on Barley Yellow Dwarf Virus

Shah, Syed Jawad Ahmad; Bashir, Muhammad Amjad; Manzoor, Naheed

doi:10.1007/978-94-007-4116-4_29

Cited by 6 publications

(11 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the most important viral stresses is barley yellow dwarf (BYD). This disease is widespread across the world, caused by viruses and transmitted by aphids (Shah et al . 2012), and can cause significant yield reductions in susceptible cultivars.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat

Silva

Evers

Kieffaber

et al. 2022

Preprint

View full text Add to dashboard Cite

Barley yellow dwarf (BYD) is one of the major viral diseases of cereals. Phenotyping BYD in wheat is extremely challenging due to similarities to other biotic and abiotic stresses. Breeding for resistance is additionally challenging as the wheat primary germplasm pool lacks genetic resistance, with most of the few resistance genes named to date originating from a wild relative species. The objectives of this study were to, i) evaluate the use of high-throughput phenotyping (HTP) from unmanned aerial systems to improve BYD assessment and selection, ii) identify genomic regions associated with BYD resistance, and iii) evaluate genomic prediction models ability to predict BYD resistance. Up to 107 wheat lines were phenotyped during each of five field seasons under both insecticide treated and untreated plots. Across all seasons, BYD severity was lower with the insecticide treatment and plant height (PTHTM) and grain yield (GY) showed increased values relative to untreated entries. Only 9.2% of the lines were positive for the presence of the translocated segment carrying resistance gene Bdv2 on chromosome 7DL. Despite the low frequency, this region was identified through association mapping. Furthermore, we mapped a potentially novel genomic region for resistance on chromosome 5AS. Given the variable heritability of the trait (0.211 0.806), we obtained relatively good predictive ability for BYD severity ranging between 0.06 0.26. Including Bdv2 on the predictive model had a large effect for predicting BYD but almost no effect for PTHTM and GY. This study was the first attempt to characterize BYD using field-HTP and apply GS to predict the disease severity. These methods have the potential to improve BYD characterization and identifying new sources of resistance will be crucial for delivering BYD resistant germplasm.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Barley yellow dwarf disease symptoms are highly variable depending on the crop, variety, time, and developmental stage when the infection occurs, aphid pressure, and environmental conditions (Shah et al . 2012; Choudhury et al .…”

Section: Introductionmentioning

confidence: 99%

Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat

Silva

Evers

Kieffaber

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…BYDV-caused barley yellow dwarf disease was first reported as a disease transmitted by aphid vectors in 1951 [ 3 ]. BYDV is known as a type member in the Luteovirus group, family Luteoviridae [ 4 ]. It is a phloem-limited virus transmitted by several cereal aphid species in a circulative-nonpropogative, persistent manner [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…BYDV is known to infect multiple grasses and cereal crops like barley and wheat, and are often referred to as barley yellow dwarf disease and wheat yellow dwarf disease [ 4 ]. Symptoms on the BYDV-infected plants can be similar to that caused by nutrient or water deficiency.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of monoclonal antibodies and serological assays specific for Barley yellow dwarf virus GAV strain

Chen

Liu

et al. 2015

Virol J

View full text Add to dashboard Cite

BackgroundBarley yellow dwarf virus (BYDV) is one of the most devastating plant viruses and belongs to a ubiquitous plant virus group. In China, four BYDV strains (GPV, GAV, PAV and RMV) have been identified based on their specific aphid vectors and serological properties. Among the four identified strains, the GAV is the most common BYDV strain in China. To diagnose, forecast of BYDV GAV, two reliable serological assays for BYDV GAV detection were established.MethodsWe purified virion from a confirmed BYDV GAV source and used it as the immunogen to produce monoclonal antibodies against the virus. Using the hybridoma technology, three highly specific murine monoclonal antibodies were produced and two serological assays [antigen-coated-plate enzyme-linked immunosorbent assay (ACP-ELISA) and dot enzyme-linked immunosorbent assay (dot-ELISA)] were established for the BYDV GAV detection.ResultsAll three monoclonal antibodies reacted strongly and specifically with the BYDV GAV strain in crude leaf extracts. Titers of the monoclonal antibodies in ascitic fluids were up to 10−7 by indirect-ELISA. These three monoclonal antibodies (18A1, 18A9 and 12A11) all belonged to the isotype IgG1, kappa light chain. The highest dilution points for the three antibodies during the ACP-ELISA using infected crude leaf extracts were 1:163,840, 1:81,920 and 1:81,920 (w/v, g · mL−1), respectively. Result of dot-ELISA showed a successful detection of BYDV GAV strain in 1:5,120 (w/v, g · mL−1) diluted wheat leaf crude extracts. Analysis of 22 field wheat leaf samples and 33 aphid samples from the Shaanxi Province in China, using the two newly developed assays confirmed the presence of BYDV GAV in about 80 % of the wheat samples and 18 % of the aphid samples.ConclusionsAll three monoclonal antibodies are highly sensitive and specific to the BYDV GAV. The two newly developed serological assays are simple and effective. These two assays, particularly the dot-ELISA, are useful for high throughput detection of BYDV GAV in host plants and aphid vectors.

show abstract

Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat

Silva

Evers

Kieffaber

et al. 2022

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Barley yellow dwarf (BYD) is one of the major viral diseases of cereals. Phenotyping BYD in wheat is extremely challenging due to similarities to other biotic and abiotic stresses. Breeding for resistance is additionally challenging as the wheat primary germplasm pool lacks genetic resistance, with most of the few resistance genes named to date originating from a wild relative species. The objectives of this study were to, i) evaluate the use of high-throughput phenotyping (HTP) to improve BYD assessment, ii) identify genomic regions associated with BYD resistance, and iii) evaluate the ability of genomic selection (GS) models to predict BYD resistance. Up to 107 wheat lines were phenotyped during each of five field seasons under both insecticide treated and untreated plots. Across all seasons, BYD severity was lower within the insecticide treatment along with increased plant height and grain yield compared to untreated entries. Only 9.2% of the lines were positive for the presence of the translocated segment carrying the resistance gene Bdv2. Despite the low frequency, this region was identified through association mapping. Furthermore, we mapped a potentially novel genomic region for BYD resistance on chromosome 5AS. Given the variable heritability of the trait (0.211–0.806), we obtained a predictive ability for BYD severity ranging between 0.06–0.26. Including the presence or absence of Bdv2 as a covariate in the GS models had a large effect for predicting BYD but almost no effect for other observed traits. This study was the first attempt to characterize BYD using field-HTP and apply GS to predict disease severity. These methods have the potential to improve BYD characterization, additionally identifying new sources of resistance will be crucial for delivering BYD resistant germplasm.

show abstract

A Review on Barley Yellow Dwarf Virus

Cited by 6 publications

References 102 publications

Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat

Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat

Development of monoclonal antibodies and serological assays specific for Barley yellow dwarf virus GAV strain

Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat

Contact Info

Product

Resources

About