A Review on High Performance Liquid Chromatography (HPLC)

Kamble, Sahil; Agrawal, Sahil; Pagade, Sagar; Patil, Rahul; Chaugule, Nilesh; Patil, Anuja

doi:10.52711/2231-5675.2023.00011

Cited by 5 publications

(1 citation statement)

References 47 publications

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“…Початково готують стандартні розчини для всіх цих сполук із відомою концентрацією, після чого проводять хроматографію. При цьому вводять у колонку кілька проб із різним вмістом сполуки, що визначається так, щоб встановити чутливість детектування даної сполуки в межах концентрацій, які відповідають припущеному вмісту цієї сполуки у невідомій пробі (Yang et al, 2022;Kamble et al, 2023).…”

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Quantitative determination of the components of the mixture – ear drops “Melavet” by the method of high-performance liquid chromatography

Parchenko,

Datsiuk,

Hunchak

et al. 2024

SMLNUVMB

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The pharmaceutical industry is one of the most regulated industries in the world, and most medicines remain safe and effective. The level of impurity and degradation products in active pharmaceutical ingredients (APIs) must be strictly controlled and meet the specifications required by international bodies. for many years, chromatography has been the method of choice for assessing the chemical purity of medicinal substances and products and has a wide range of applications in the pharmaceutical industry, from research and development to laboratory quality control. The ability to integrate mass spectrometry into the chromatography workflow has been a real breakthrough in the science of separation, finding many inscriptions, especially in the field of pharmaceutical analysis. Therefore, the relevance of the research lies in finding the quantity of the composition of the created mixture – ear drops “Melavet” by the HPLC method with mass detection of the initial components. The purpose of the work is to create analytical methods for determining the active components of the mixture when they are present in the sample and interact with each other. Materials and methods: Chromato-mass-spectrometric studies, high-performance liquid chromatography (HPLC, LCMS). LCMS device: Agilent 1200 Quaternary DAD HPLC System (degasser, binary pump, autosampler, column thermostat, diode-matrix detector; single-quadrupole mass spectrometer Agilent 1100 LCMSD SL instrument; OpenLAB CDS Software. For the new combined veterinary test of ear drops “Melavet” 10 ml, containing various components, it is necessary to develop optimal methods of standardization. For this, 6 solutions of the external standard of each component were prepared. Based on the research, a method was developed for the simultaneous determination of the components in the model mixture ear drops “Melavet” 10 ml by the method of high-performance liquid chromatography using the same mobile and stationary phases, as well as similar chromatographic conditions, which were used during the study of individual samples of the external standard. When analyzing the chromatographic data, the presence of dioxidin in the mixture was fully consistent. In comparison with the external standards of each component, there are separate icons of substances on the chromatogram of the mixture, which contain information about the purity of the components and the interaction between them. During the research, a highly sensitive technique for the simultaneous determination of the components of the model mixture “Melavet” ear drops 10 ml by the method of high-performance liquid chromatography was developed. The optimal conditions for the simultaneous determination of the components of the mixture in one sample are selected. It was established that there is no interaction between the components of the mixture when they are simultaneously in the sample solution.

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Section: вступunclassified

Quantitative determination of the components of the mixture – ear drops “Melavet” by the method of high-performance liquid chromatography

Parchenko,

Datsiuk,

Hunchak

et al. 2024

SMLNUVMB

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Rapid Development and Validation of Atoltivimab, Maftivimab and Odesivimab in Pharmaceutical Dosage form by using the RP-HPLC Method

Rao,

Sahoo

et al. 2024

CPA

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Background: The reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed for the quantitative measurement of monoclonal antibodies (Maftivimab, Atoltivimab, and Odesivimab) in the pharmaceutical dosage form. The Food and Drug Administration (FDA) has approved these monoclonal antibodies for the treatment of Zaire ebolavirus infection in adults. Methods: Maftivimab, Atoltivimab, and Odesivimab were separated chromatographically on the Waters Alliance-e2695 platform using the Luna Phenyl Hexyl (250 x 4.6 mm, 5 μm) column and a mobile phase made up of Acetonitrile (ACN) and ortho-phosphoric acid (OPA) buffer in a ratio of 70:30 (v/v). Results: The flow rate was 1.0 ml/min, and a photodiode array (PDA) detector operating at room temperature was used to measure absorption at 282 nm. For Maftivimab, Atoltivimab, and Odesivimab, the theoretical plates were not less than 2000, and the tailing factor shouldn't be greater than 2, accordingly. All measurements have a constant relative standard deviation of peak areas that is less than 2.0. Conclusion: The suggested procedure was approved following the International Conference on Harmonisation (ICH) recommendations. When used for the quantitative analysis of Maftivimab, Atoltivimab, and Odesivimab, the approach was found to be straightforward, affordable, appropriate, exact, accurate, and robust.

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A Review on High Performance Liquid Chromatography (HPLC)

Priya M. Dandekar,

Mayuri G. Zore,

Aasma I. Sheikh

et al. 2024

IJARSCT

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High-performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples. It is the most versatile, safest, dependable, and fastest chromatographic technique for the quality control of drug components. This article was prepared with a review different aspects of HPLC, such as principle types, instrumentation, and application. High- Performance Liquid Chromatography has played a significant role in clinical laboratories for the separation and quantitation of biomarkers in different body fluids. The development of HPLC involves four basic steps; scouting, optimization, robustness testing, and validation. The technique is used to analyze drugs and medicines for their purity and to maintain the highest standards for pharmaceutical products with the end goal of helping patients with medical issues. Method of validation is the process used to confirm that the analytical procedure employed for a specific test is suitable for its intended use. Validation High-Performance Liquid Chromatography method as per ICH guidelines covers all the performance characteristics of validation, like Accuracy, precision, specificity, linearity, range, limit of detection, limit of quantification, robustness, system suitability the testing. The limitation of High-Performance Liquid Chromatography methods of development, public health importance, and validation is the automated process becomes complicated, has low separation power, and is expensive but High-Performance Liquid Chromatography is the modern diagnostic technique is used in all sectors

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A Review on High Performance Liquid Chromatography (HPLC)

Cited by 5 publications

References 47 publications

Quantitative determination of the components of the mixture – ear drops “Melavet” by the method of high-performance liquid chromatography

Quantitative determination of the components of the mixture – ear drops “Melavet” by the method of high-performance liquid chromatography

Rapid Development and Validation of Atoltivimab, Maftivimab and Odesivimab in Pharmaceutical Dosage form by using the RP-HPLC Method

A Review on High Performance Liquid Chromatography (HPLC)

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