Continuous dams may lead to great variation in greenhouse gas (GHG) emissions from rivers, which contribute more uncertainty to regional carbon balance. This study is among the first to determine water–air interface GHGs (CO2, CH4, and N2O) in a river with continuous dams in plateau city. Combined static-chamber gas and meteorological chromatography were utilized to monitor the GHGs emission flux at the water–air interface within four continuous dams in the Huoshaogou River in the Qinghai–Tibet Plateau, China. A variation coefficient (VC) and amplification coefficient (AC) were designed to detect the influence of continuous dams on GHG emissions. Results indicate that (1) cascade dams presented an amplifying effect on GHGs emissions from the water-air interface. The VCs of three types of GHGs are 3.7–6.7 times higher than those of the undammed area. The ACs of three types of GHGs are 2.7–4.1 times larger than environmental factors; (2) the average GHG emission fluxes in some dams are higher than that of the first dam, indicating that an amplifying effect may have been accumulated by some continuous dams; (3) EC, pH, Twater, Tair and TDS are found to be principle influencing factors of GHG emission and light intensity, Twater, TOC (plant), TN (sediment) and TOC (sediment) are found to be associated with accumulative changes in GHG emission.