A revision of the genus<i>Orosius</i>Distant (Hemiptera: Cicadellidae) based on male genitalia and DNA barcoding

Fletcher, Murray J.; Löcker, Holger; Mitchell, Andrew; Gopurenko, David

doi:10.1111/aen.12224

Cited by 13 publications

(10 citation statements)

References 44 publications

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“…We included the mitochondrial DNA from Orosius sp. as the known outgroup for Nesophrosyne ( Bennett and O’Grady, 2012 ; Fletcher et al., 2017 ). We also tested co-cladogenesis between host and symbiont by extracting and aligning all Nasuia and Sulcia genes.…”

Section: Methodsmentioning

confidence: 99%

A complex interplay of evolutionary forces continues to shape ancient co-occurring symbiont genomes

Vasquez

Bennett

2022

iScience

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Section: Methodsmentioning

confidence: 99%

A complex interplay of evolutionary forces continues to shape ancient co-occurring symbiont genomes

Vasquez

Bennett

2022

iScience

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“…A total of 1482 specimens based on DNA barcodes of Nearctic Auchenorrhyncha (Insecta, Hemiptera) were studied by Foottit et al (2014). The boundaries of seven closely related species of the evacanthine leafhopper genus Bundera (Cicadellidae, Evacanthinae) based on DNA barcoding, morphology and hyperspectral reflectance profiling was investigated by Wang et al (2016), and a revision of the genus Orosius (Cicadellidae, Deltocephalinae, Opsiini) based on morphological and DNA barcoding was undertaken by Fletcher et al (2017). Although, DNA barcoding research has been applied to these groups of leafhoppers, until now, a few molecular data are available for Deltocephalus .…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding of Deltocephalus Burmeister leafhoppers (Cicadellidae, Deltocephalinae, Deltocephalini) in China

Zhang

Duan

2019

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We investigated the feasibility of using the DNA barcode region in identifying Deltocephalus from China. Sequences of the barcode region of the mitochondrial COI gene were obtained for 98 specimens (Deltocephalusvulgaris – 88, Deltocephaluspulicaris – 5, Deltocephalusuncinatus – 5). The average genetic distances among morphological and geographical groups of D.vulgaris ranged from 0.9% to 6.3% and among the three species of Deltocephalus ranged from 16.4% to 21.9% without overlap, which effectively reveals the existence of a “DNA barcoding gap”. It is important to assess the status of these morphological variants and explore the genetic variation among Chinese populations of D.vulgaris because the status of this species has led to taxonomic confusion because specimens representing two distinct morphological variants based on the form of the aedeagus are often encountered at a single locality. Forty-five haplotypes (D.vulgaris – 36, D.pulicaris – 5, D.uncinatus – 4) were defined to perform the phylogenetic analyses; they revealed no distinct lineages corresponding either to the two morphotypes of D.vulgaris or to geographical populations. Thus, there is no evidence that these variants represent genetically distinct species.

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“…as potentially of epidemiological significance for spread of this phytoplasma to crops in northern Australia because it grows in close association with sweetpotato and supports high levels of the supposed vector insect O. argentatus . Fletcher, et al 29. provide evidence that O. argentatus is a valid species and distinct from O. orientalis with which it had previously been synonymised by Kwon and Lee30.…”

Section: Discussionmentioning

confidence: 99%

“…PCR for DNA barcoding33 of insects targeted a 667 base pair (bp) portion of the 5′ mitochondrial cytochrome c oxidase I (COI) gene using primers described in Fletcher, et al 29…”

Section: Methodsmentioning

confidence: 99%

Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa)

Gopurenko

Fletcher

Liu

et al. 2016

Sci Rep

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Phytoplasmas are a group of insect-vectored bacteria responsible for disease in many plant species worldwide. Among the crop species affected is the economically valuable forage species lucerne. Here we provide comprehensive molecular evidence for infection in multiple lucerne plants by a phytoplasma not previously known from this plant species. This phytoplasma had a >99% genetic similarity to an unclassified 16S rRNA subgroup previously reported as Stylosanthes little leaf from Stylosanthes spp. and was genetically and symptomatically distinct from a co-occurring but less common 16SrIIA group phytoplasma. Neighbour-joining analyses with publicly available sequence data confirmed the presence of two distinct phytoplasma lineages in the plant population. No PCR detections were made among 38 individuals of 12 co-occurring weed species. Sequence analysis revealed that all nine PCR detections from among 106 individuals of five Hemiptera insect species from the site, three of which had previously been reported as likely vectors, were false positives. This study demonstrates the importance of sequencing to complement PCR detection and avoid potentially inaccurate conclusions regarding vectors, highlights that sampling over a wide spatio-temporal scale is important for vector and alternative host studies, and extends to eight the number of phytoplasma 16 Sr groups known from lucerne.

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A revision of the genusOrosiusDistant (Hemiptera: Cicadellidae) based on male genitalia and DNA barcoding

Cited by 13 publications

References 44 publications

A complex interplay of evolutionary forces continues to shape ancient co-occurring symbiont genomes

A complex interplay of evolutionary forces continues to shape ancient co-occurring symbiont genomes

DNA barcoding of Deltocephalus Burmeister leafhoppers (Cicadellidae, Deltocephalinae, Deltocephalini) in China

Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa)

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