A revisit of mutation induction by gamma rays in rice (Oryza sativa L.): implications of microsatellite markers for quality control

Fu, Haidong; Li, Youfa; Shu, Qingyao

doi:10.1007/s11032-008-9173-7

Cited by 39 publications

(35 citation statements)

References 13 publications

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“…However, these types of events do not appear to be frequent during radiation treatment. 37 Analysis of 10 different SSR loci in 100 different mutant cowpea M1 individuals originating from the same parent also showed in our study no detectable variation in fragment size in our analyzed lines. Results reported here are therefore consistent with previous findings 37 in rice where induced mutants had SSR profiles identical to their parents.…”

supporting

confidence: 73%

“…37 Analysis of 10 different SSR loci in 100 different mutant cowpea M1 individuals originating from the same parent also showed in our study no detectable variation in fragment size in our analyzed lines. Results reported here are therefore consistent with previous findings 37 in rice where induced mutants had SSR profiles identical to their parents. Any possible detectable variations due to outcrossing, which results in SSR polymorphisms, could then have been interpreted as equivalent to the results found for salt-tolerant sorghum, where SSRs detected genetic changes induced by gamma rays 38 but subsequently interpreted as resulting from outcrossing.…”

supporting

confidence: 73%

“…This result is consistent with the known effects of gamma rays on the growth of M1 plants, which include reduction of fertility, seed set, plant height, and abnormal growth forms, such as chlorosis and curly leaves, with a correlation between dose and altered phenotype. 37,40 In summary, our study has shown that in cowpea a large proportion of induced nonlethal mutations might occur within nuclear plastid insertions and a relatively high repair rate occurs in mutated plants in the progeny. The high frequency of alterations in this compartment of the nuclear genome is consistent with the suggestion that the regions in which chloroplast sequences integrate into the genome are particularly labile.…”

mentioning

confidence: 75%

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Analysis of radiation-induced genome alterations in Vigna unguiculata

Vyver¹,

Vorster²,

Kunert³

et al. 2011

RRB

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Abstract:Seeds from an inbred Vigna unguiculata (cowpea) cultivar were gamma-irradiated with a dose of 180 Gy in order to identify and characterize possible mutations. Three techniques, ie, random amplified polymorphic DNA, microsatellites, and representational difference analysis, were used to characterize possible DNA variation among the mutants and nonirradiated control plants both immediately after irradiation and in subsequent generations. A large portion of putative radiation-induced genome changes had significant similarities to chloroplast sequences. The frequency of mutation at three of these isolated polymorphic regions with chloroplast similarity was further determined by polymerase chain reaction screening using a large number of individual parental, M1, and M2 plants. Analysis of these sequences indicated that the rate at which various regions of the genome is mutated in irradiation experiments differs significantly and also that mutations have variable "repair" rates. Furthermore, regions of the nuclear DNA derived from the chloroplast genome are highly susceptible to modification by radiation treatment. Overall, data have provided detailed information on the effects of gamma irradiation on the cowpea genome and about the ability of the plant to repair these genome changes in subsequent plant generations.

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supporting

confidence: 73%

supporting

confidence: 73%

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confidence: 75%

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Analysis of radiation-induced genome alterations in Vigna unguiculata

Vyver¹,

Vorster²,

Kunert³

et al. 2011

RRB

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“…Mutation techniques have been used for generating vast genetic variations and breeding thousands of new crop varieties during the past decades (Fu et al, 2008). According to Sharma (1986), the crop plant characteristics that have been improved by mutation breeding are generally those that have either not found favor with natural selection or were not achieved during previous plant breeding efforts.…”

Section: Introductionmentioning

confidence: 99%

Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation

Ling

Ung

Hussein

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphological and biochemical responses of Oryza sativa toward different doses of carbon ion beam irradiation. Methods: In this study, the dry seeds of O. sativa were irradiated at 0, 20, 40, 60, 80, 100, and 120 Gy, followed by in-vitro germination under controlled conditions. Morphological and biochemical studies were conducted to investigate the morphological and physiological responses of O. sativa towards ion beam irradiation. Results: The study demonstrated that low doses (10 Gy) of ion beam have a stimulating effect on the height, root length, and fresh weight of the plantlets but not on the number of leaves. Meanwhile, doses higher than 10 Gy caused reductions in all the morphological parameters studied as compared to the control samples. The highest total soluble protein content [(2.11±0.47) mg/g FW] was observed in plantlets irradiated at 20 Gy. All irradiated plantlets were found to have 0.85% to 58.32% higher specific activity of peroxidase as compared to the control samples. The present study also revealed that low doses of ion beam (10 and 20 Gy) had negligible effect on the total chlorophyll content of O. sativa plantlets while 40 Gy had a stimulating effect on the chlorophyll content. Plantlets irradiated between 40 to 120 Gy were shown to be 0.38% to 9.98% higher in total soluble nitrogen content which, however, was not significantly different from the control samples. Conclusions: Carbon ion beam irradiation administered at low to moderate doses of 10 to 40 Gy may induce O. sativa mutants with superior characteristics.

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“…According to the IAEA database, there are more than 2500 mutant varieties of more than 170 different species [7]. There are many reports of improved morphological and physiological characteristics in cereals, grain legumes, oil seeds, fiber crops, vegetables and ornamentals following mutation induction, with gamma rays being the preferred agent, and plant type and yield are the traits most commonly reported [8][9][10]. Although the quality of many crops has been improved thru mutation induction [11], to the best of our knowledge, this technique has not been applied in quinoa to improve its agricultural performance in the marginal conditions of the Peruvian highlands but it was applied in barley and Amaranthus [12].…”

Section: Introductionmentioning

confidence: 99%

Developing Genetic Variability of Quinoa (<i>Chenopodium quinoa</i> Willd.) with Gamma Radiation for Use in Breeding Programs

Gómez-Pando¹,

Barra²

2013

AJPS

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Quinoa (Chenopodium quinoa Willd.) is a staple food produced mainly by small-scale subsistence farmers in Peru's highland. Dry seeds (cv. Pasankalla) were irradiated with doses of 150 Gy, 250 Gy and 350 Gy. In the M 1 generation, the germination process was delayed with increasing radiation dose; seedling height, root length and leaf development were most reduced at 250 Gy and at 350 Gy, no plants survived. In M 2 , the maximum spectrum of chlorophyll mutations corresponded to 150 Gy and the maximum frequency to 250 Gy. The chlorine mutation was predominant, followed by xantha. Changes were registered for branch number, pedicel length, plant height, life-cycle duration, stem and foliage colour, and leaf morphology at the two doses, with improvements in plant type. More than one mutation per plant was found, especially at 250 Gy. In M 3 , the same spectrum of mutations was observed, along with a valuable change in grain colour.

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A revisit of mutation induction by gamma rays in rice (Oryza sativa L.): implications of microsatellite markers for quality control

Cited by 39 publications

References 13 publications

Analysis of radiation-induced genome alterations in Vigna unguiculata

Analysis of radiation-induced genome alterations in Vigna unguiculata

Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation

Developing Genetic Variability of Quinoa (<i>Chenopodium quinoa</i> Willd.) with Gamma Radiation for Use in Breeding Programs

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A revisit of mutation induction by gamma rays in rice (Oryza sativa L.): implications of microsatellite markers for quality control

Cited by 39 publications

References 13 publications

Analysis of radiation-induced genome alterations in Vigna unguiculata

Analysis of radiation-induced genome alterations in Vigna unguiculata

Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation

Developing Genetic Variability of Quinoa (&lt;i&gt;Chenopodium quinoa&lt;/i&gt; Willd.) with Gamma Radiation for Use in Breeding Programs

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Product

Resources

About

Developing Genetic Variability of Quinoa (<i>Chenopodium quinoa</i> Willd.) with Gamma Radiation for Use in Breeding Programs