A revisit to the retention mechanism of hydrophilic interaction liquid chromatography using model organic compounds

Karatapanis, Andreas E.; Fiamegos, Yiannis C.; Stalikas, Constantine D.

doi:10.1016/j.chroma.2011.02.069

Cited by 125 publications

(86 citation statements)

References 47 publications

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“…In HILIC, a relatively hydrophobic high organic content mobile phase is used to elute analytes through a hydrophilic column. 42,46,47,50,[53][54][55][56][57] The mechanism is the extent of partitioning of analytes between a pseudostagnant aqua layer formed at the surface of the stationary phase and the mobile phase, as confirmed by McCalley and Neue, and Dinh et al 58,59 The result is that retention favours the more hydrophilic analytes as confirmed by the negative slope of Fig. 4.…”

Section: Ascentis Hilic Columnsupporting

confidence: 52%

“…45,47,56,[60][61][62][63] Studies by Li and Huang 62 showed better separation when MeCN was used as the mobile phase compared to MeOH, THF and isopropanol. Attempts by Dinh et al 58 to admixture THF with the MeCN-containing mobile phase reduced the extent of formation of the water enriched layer on the stationary phase.…”

Section: The Moderately Polar Aprotic Diluent For Analyte Injectionmentioning

confidence: 99%

“…While the recommendations guided by the viscous fingering phenomenon are that the injection solvent must be as close to the mobile phase organic composition as possible with a minimal 50 % MeCN composition, such declarations have been based on comparison of MeCN with polar protic solutions like H 2 O and MeOH. 56,60,64 It was noted that most publications follow this protocol and rarely report or optimize their injection diluents. Controversies arise considering that the polar protic MeOH and H 2 O are the solvents of choice for the extraction of polar compounds from biological matrices.…”

Section: The Moderately Polar Aprotic Diluent For Analyte Injectionmentioning

confidence: 99%

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Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

et al. 2016

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The number of hallucinogenic compounds that have been separated simultaneously by liquid chromatography is limited. This research aimed to identify a column(s) that can allow for separation of several hallucinogens. The extent of separation of seven polar hallucinogenic tryptamine and phenethylamine derived alkaloids containing a basic N atom that becomes protonated at low pH values were investigated on five reverse-based columns and one hydrophilic interaction liquid chromatography (HILIC) column. An RP-phenyl and a negatively charged fused core HILIC were identified and recommended as effective columns in this regard. This research is the first to introduce a HILIC column in separation of hallucinogenic alkaloids and to simultaneously study the separation of the two principal psychoactive agents, muscimol and psilocin. The results of this study showed that better separation in HILIC columns can be achieved if an aprotic diluent of relatively lower polarity is used to introduce analytes into a mobile phase of higher polarity. Tetrahydrofuran was recommended where acetonitrile is used as the organic component of the mobile phase.

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Section: Ascentis Hilic Columnsupporting

confidence: 52%

Section: The Moderately Polar Aprotic Diluent For Analyte Injectionmentioning

confidence: 99%

Section: The Moderately Polar Aprotic Diluent For Analyte Injectionmentioning

confidence: 99%

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Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

et al. 2016

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“…Although both the linear reversed phase (semi-log) (Eq. (1)) and the normal phase (log-log) retention relationships have been used in literature to model HILIC retention [15], the dependency of ln(k) on both ϕ and log(ϕ) in the HILIC separation mode does not follow a strict linear relationship [15,16]. Based on these observations, Jin et al proposed a mixed model combining partitioning and adsorption terms (Eq.…”

Section: Introductionmentioning

confidence: 99%

Use of individual retention modeling for gradient optimization in hydrophilic interaction chromatography: Separation of nucleobases and nucleosides

Tyteca

Guillarme

Desmet

2014

Journal of Chromatography A

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a b s t r a c tIn this study, the separation of twelve nucleobases and nucleosides was optimized via chromatogram simulation (i.e., prediction of individual retention times and estimation of the peak widths) with the use of an empirical (reversed-phase) non-linear model proposed by Neue and Kuss. Retention time prediction errors of less than 2% were observed for all compounds on different stationary phases. As a single HILIC column could not resolve all peaks, the modeling was extended to coupled-column systems (with different stationary phase chemistries) to increase the separation efficiency and selectivity. The analytical expressions for the gradient retention factor on a coupled column system were derived and accurate retention time predictions were obtained (<2% prediction errors in general). The optimized gradient (predicted by the optimization software) included coupling of an amide and an pentahydroxy functionalized silica stationary phases with a gradient profile from 95 to 85%ACN in 6 min and resulted in almost baseline separation of the twelve nucleobases and nucleosides in less than 7 min. The final separation was obtained in less than 4 h of instrument time (including equilibration times) and was fully obtained via computer-based optimization. As such, this study provides an example of a case where individual retention modeling can be used as a way to optimize the gradient conditions in the HILIC mode using a non-linear model such as the Neue and Kuss model.

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“…On the other hand, if the silanol effect of bonded-phase silica gel is eliminated, the retention mechanisms of hydrophilic-interaction liquid chromatography (HILIC) are hydrogen bonding (HB) and Lewis acid-base (charge-transfer) interactions that are dependent upon the chemical structures of the packing materials and analytes [3]. The retention mechanism of HILIC-mode liquid chromatography was reviewed [4][5][6][7][8][9][10]. The chromatographic behavior of selected compounds using silica-based stationary phases was studied, and it was concluded that the description of either partition or adsorption mechanisms were inconclusive in HILIC.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Explanation of Retention Mechanisms of Hydrophobic and Hydrophilic-Interaction Liquid Chromatography-Inductive Effect of Alkyl Chain

Hanai

2017

Separations

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Abstract:The basic retention mechanisms of reversed-phase and hydrophilic-interaction liquid chromatography (HILIC) were quantitatively analyzed using simple molecular mechanics (MM) calculations. The model bonded phases were composed of an alkyl chain bonded to siloxane-like molecules. The head of the alkyl chain was a hydroxyl group, used to study retention mechanisms of HILIC. The standard analytes were benzoic acid, aniline, benzene, water, and acetonitrile. The molecular interaction (MI) energy values of the van der Waals (VW) force were predominant for the reversed-phase chromatography, and those of hydrogen bonding (HB) were predominant for HILIC. Up to 5 units of methylene affected the MI.Keywords: retention mechanism; hydrophobic interaction; hydrophilic interaction; inductive effect of alkyl chain; computational chemistry

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A revisit to the retention mechanism of hydrophilic interaction liquid chromatography using model organic compounds

Cited by 125 publications

References 47 publications

Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

Use of individual retention modeling for gradient optimization in hydrophilic interaction chromatography: Separation of nucleobases and nucleosides

Quantitative Explanation of Retention Mechanisms of Hydrophobic and Hydrophilic-Interaction Liquid Chromatography-Inductive Effect of Alkyl Chain

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