A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer

Hanke, Merle; Hoefig, Kai P.; Merz, Hartmut; Feller, Alfred C.; Kausch, Ingo; Jocham, Dieter; Warnecke, Jens M.; Sczakiel, Georg

doi:10.1016/j.urolonc.2009.01.027

Cited by 581 publications

(371 citation statements)

References 25 publications

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“…Because it is possible to assess miRNA expression patterns using urine (35) or blood serum (36), the identified deregulated miRNAs pose the potential of being valuable tumor markers for the diagnosis of prostate cancer. It is known that the myosin VI expression level is even more predictive for the aggressiveness of prostate cancer (33).…”

Section: Discussionmentioning

confidence: 99%

The MicroRNA Profile of Prostate Carcinoma Obtained by Deep Sequencing

Szczyrba

Löprich²,

Wach³

et al. 2010

Molecular Cancer Research

208

207

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Prostate cancer is a leading cause of tumor mortality. To characterize the underlying molecular mechanisms, we have compared the microRNA (miRNA) profile of primary prostate cancers and noncancer prostate tissues using deep sequencing. MiRNAs are small noncoding RNAs of 21 to 25 nucleotides that regulate gene expression through the inhibition of protein synthesis. We find that 33 miRNAs were upregulated or downregulated >1.5-fold. The deregulation of selected miRNAs was confirmed by both Northern blotting and quantitative reverse transcription-PCR in established prostate cancer cell lines and clinical tissue samples. A computational search indicated the 3′-untranslated region (UTR) of the mRNA for myosin VI (MYO6) as a potential target for both miR-143 and miR-145, the expression of which was reduced in the tumor tissues. Upregulation of myosin VI in prostate cancer was previously shown by immunohistochemistry. The level of MYO6 mRNA was significantly induced in all primary tumor tissues compared with the nontumor tissue from the same patient. This finding was matched to the upregulation of myosin VI in established prostate cancer cell lines. In luciferase reporter analysis, we find a significant negative regulatory effect on the MYO6 3′UTR by both miR-143 and miR-145. Mutation of the potential binding sites for miR-143 and miR-145 in the MYO6 3′ UTR resulted in a loss of responsiveness to the corresponding miRNA. Our data indicate that miR-143 and miR-145 are involved in the regulation of MYO6 expression and possibly in the development of prostate cancer. Mol Cancer Res; 8(4); 529-38. ©2010 AACR.

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Section: Discussionmentioning

confidence: 99%

The MicroRNA Profile of Prostate Carcinoma Obtained by Deep Sequencing

Szczyrba

Löprich²,

Wach³

et al. 2010

Molecular Cancer Research

208

207

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“…Studies conducted by several research groups have confirmed the presence of miRNAs in various body fluids in humans, including serum [49][50][51], plasma [49,50], saliva [52], urine [53], milk [54], cerebrospinal fluid [55] and seminal fluid [56]. In cancer, there is a distinct relationship between the type of biological material and the original location of the neoplasm (e.g.…”

Section: Extracellular Mirnamentioning

confidence: 99%

miRNAs as Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer: Current Perspectives

Florczuk¹,

Szpechcinski²,

Chorostowska‐Wynimko³

2017

Targ Oncol

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Lung cancer is the most common cancer worldwide. Up to 85% of lung cancer cases are diagnosed as nonsmall cell lung cancer (NSCLC). The effectiveness of NSCLC treatment is expected to be improved through the implementation of robust and specific biomarkers. MicroRNAs (miRNAs) are small, non-coding molecules that play a key role in the regulation of basic cellular processes, including differentiation, proliferation and apoptosis, by controlling gene expression at the post-transcriptional level.

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“…Nevertheless, each of the approaches has its own specific advantages and disadvantages. Recently, the findings of miRNAs in body fluids, such as serum, urine and colostrum (Hanke et al, 2010;Link et al, 2010) have strengthened investigations into the use of miRNAs as non-invasive biomarkers of disease and a therapeutic response in a range of cancers including lung cancer, ovarian cancer, colorectal cancer, renal cancer, glioblastoma, prostate cancer and breast cancer (Cortez et al, 2012). In recent times, Lin et al (2014) characterized miRNAs expression profiles in cervical cancer of Uygur women to identify the biomarkers for early diagnosis.…”

Section: Micrornas As Potential Biomarkers For Cancer Diagnosis and Pmentioning

confidence: 99%

MicroRNAs: Biogenesis, Roles for Carcinogenesis and as Potential Biomarkers for Cancer Diagnosis and Prognosis

Kavitha¹,

Vijayarathna²,

Jothy³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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MicroRNAs (miRNAs) are short non-coding RNAs of 20-24 nucleotides that play important roles in carcinogenesis. Accordingly, miRNAs control numerous cancer-relevant biological events such as cell proliferation, cell cycle control, metabolism and apoptosis. In this review, we summarize the current knowledge and concepts concerning the biogenesis of miRNAs, miRNA roles in cancer and their potential as biomarkers for cancer diagnosis and prognosis including the regulation of key cancer-related pathways, such as cell cycle control and miRNA dysregulation. Moreover, microRNA molecules are already receiving the attention of world researchers as therapeutic targets and agents. Therefore, in-depth knowledge of microRNAs has the potential not only to identify their roles in cancer, but also to exploit them as potential biomarkers for cancer diagnosis and identify therapeutic targets for new drug discovery.

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A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer

Cited by 581 publications

References 25 publications

The MicroRNA Profile of Prostate Carcinoma Obtained by Deep Sequencing

The MicroRNA Profile of Prostate Carcinoma Obtained by Deep Sequencing

miRNAs as Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer: Current Perspectives

MicroRNAs: Biogenesis, Roles for Carcinogenesis and as Potential Biomarkers for Cancer Diagnosis and Prognosis

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