A robust OFF-ON fluorescent biosensor for detection and clearance of bacterial endotoxin by specific peptide based aggregation induced emission

Near-Infrared-Fluorescent Probe for Turn-On Lipopolysaccharide Analysis Based on PEG-Modified Gold Nanorods with Plasmon-Enhanced Fluorescence

“…With the as-prepared plasmonenhanced probe, the detection limit was 3.85 ng/mL. Compared with the previously reported immunoassay method 15,16 with an antibody as the recognition component or colorimetry method, 42,43 as well as the fluorescence probe 10,44,45 (Table S1), our as-prepared probe with a peptide as the recognition component showed a shorter detection time, wider linear range, and a lower detection limit. And the antibody in the immunoassay method is easy to denature and showed lower stability than the peptide or aptamer.…”

“…And presence of glucose can lead to false-positive results, greatly limiting its application in the biomedical field. Therefore, biosensors independent of Limulus reagent have been widely studied. − Wang et al developed a colorimetric and fluorometric probe based on 3-phenylthiophene-based water-soluble copolythiophenes, with the emission wavelength of 600 nm, which can detect LPS at the picomolar level . Liedberg et al developed an LPS sensor by assembling tetramethylrhodamine-labeled LPS-binding peptides on graphene oxide, which can be used as a turn-on fluorescence probe .…”

Environmental protection based on the nanobiosensing of bacterial lipopolysaccharides (LPSs): material and method overview

“…The developed platform can achieve LPS sensing in a wide linear range of 0.1 to 1 μM and good LOD (6.97 nM). 101 Ultra-sensitive detection of LPS based on combined amplification of dual enzymes was designated recently. With the effective signal amplification, the bio-system revealed high sensitivity with wide range linearity (2.5–1000 pg mL −1 ) and an acceptable LOD1 (pg mL −1 ).…”

Insights into AIE materials: A focus on biomedical applications of fluorescence

“…Owing to their simple structure, low background noise, excellent photostability, and admirable biocompatibility, AIE molecules are beginning to establish themselves as significant tools in the areas of life science for biosensor ( Zhang L. et al, 2020b ; He et al, 2020 ) and comprehending bioprocesses ( Wang et al, 2015 ; Gu et al, 2017 ; Gao F. et al, 2020 ). To elucidate the biological functions of fluorescent probes or drugs ( Wang Z. et al, 2020 ), a comprehensive understanding of their intracellular division is of vital importance ( Dong Y. et al, 2020 ; Tang et al, 2020 ). The most common pattern for detecting biological macromolecules is functionalizing with small hydrophilic groups such as amine groups, sulfonic acid groups, or hydrophilic peptide chains to greatly improve the water solubility of AIE molecules and then utilizing the electrostatic interaction, hydrophobic interaction, and receptor-assisted interactions between the water-soluble AIE molecules and biological macromolecules to turn on/off the fluorescence and achieve the detection ( Xu et al, 2012 ).…”