A role for biosynthetic CdS quantum dots in extracellular electron transfer of Saccharomyces cerevisiae

Wu, Ranran; Wang, Chao; Shen, Jing; Zhao, Feng

doi:10.1016/j.procbio.2015.10.005

Cited by 30 publications

(16 citation statements)

References 27 publications

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“…CdS nanoparticles produced by this Antarctic strain showed a zeta potential value (lower than −20 mV) indicative of high stability in aqueous solutions, with nanocrystals produced with Met being the most stable (Kuznetsova and Rempel, 2015). The average size of the nanoparticles produced with Cys (∼2 nm) and Met (∼16 nm), determined from TEM images, were consistent with the size of QDs biosynthesized by other microorganisms (Chen et al, 2009; Khachatryan et al, 2009; Syed and Ahmad, 2013; Wu et al, 2015; Al-Shalabi and Doran, 2016; Tandon and Vats, 2016; Wang et al, 2018; Bruna et al, 2019). TEM as a technique for characterizing nanomaterials enables the visualization of the shape and size of the nanoparticles by providing direct images of nanomaterials at a spatial resolution (Lina et al, 2014).…”

Section: Discussionsupporting

confidence: 75%

“…To achieve this, research on the use of natural sources such as biological systems becomes essential. Biological production or biosynthesis of metal nanoparticles has been widely studied through the use of microorganisms, such as fungi, yeast and bacteria (Wu et al, 2015; Chakraborty et al, 2018; Qin et al, 2018). Due to the convenience of this method, a cost-effective, environmentally friendly and highly biocompatible alternative based on the use of bacterial cells has emerged (Monrás et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

et al. 2019

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Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H 2 S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1 T : megL – (unable to produce MeSH from Met) and mddA – (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL – strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA – strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur source. This work represents the first report that directly associates the production of MeSH with the bacterial synthesis of QDs, thus revealing the importance of different VSCs in the biological generation of metal sulfide nanostructures.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

et al. 2019

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“…With the QDs present within the cells, the electrochemical character of the yeast now became dependent on the illumination of the cells and the redox current increased under illumination. 1020 Other examples where cells use semiconductors (though not QDs) to transfer electrons have also been described. 1021 Of special interest in these examples is that the proteome of these cellular systems appears to be modified, which suggests an entryway for bioengineering and optimization of future systems.…”

Section: Electrode Systems Incorporating Quantum Dotsmentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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Luminescent semiconductor quantum dots (QDs) are one of the more popular nanomaterials currently utilized within biological applications. However, what is not widely appreciated is their growing role as versatile energy transfer (ET) donors and acceptors within a similar biological context. The progress made on integrating QDs and ET in biological configurations and applications is reviewed in detail here. The goal is to provide the reader with (1) an appreciation for what QDs are capable of in this context, (2) how this field has grown over a relatively short time span, and, in particular, (3) how QDs are steadily revolutionizing the development of new biosensors along with a myriad of other photonically active nanomaterial-based bioconjugates. An initial discussion of QD materials along with key concepts surrounding their preparation and bioconjugation is provided given the defining role these aspects play in the QDs ability to succeed in subsequent ET applications. The discussion is then divided around the specific roles that QDs provide as either Förster resonance energy transfer (FRET) or charge/electron transfer donor and/or acceptor. For each QD-ET mechanism, a working explanation of the appropriate background theory and formalism is articulated before examining their biosensing and related ET utility. Other configurations such as incorporation of QDs into multistep ET processes or use of initial chemical and bioluminescent excitation are treated similarly. ET processes that are still not fully understood such as QD interactions with gold and other metal nanoparticles along with carbon allotropes are also covered. Given their maturity, some specific applications ranging from in vitro sensing assays to cellular imaging are separated and discussed in more detail. Finally a perspective on how this field will continue to evolve is provided.

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“…Few advantages of using a fungal-mediated green approach to the nanoparticle synthesis are as follows: economic capability and ease in scale-up and handling, thus making it possible to obtain biomass easily for processing, and large-scale production of different extracellular enzymes [15]. It has been reported that five yeast strains, namely, Candida glabrata for hexamers that are intracellularly and extracellularly synthesized [16], Candida glabrata for intracellularly synthesized compounds [17], Schizosaccharomyces pombe [6][7][8]17], Trichosporon jirovecii [18], and Saccharomyces cerevisiae [19], can produce CdSNPs when cultured within the presence of cadmium salts. CdSQD nanoparticles were also extracellularly biosynthesized by Fusarium sp.…”

Section: Introductionmentioning

confidence: 99%

Anticancer and Antibacterial Activity of Cadmium Sulfide Nanoparticles byAspergillus niger

Al-Saggaf

El-Baz

Badawy

et al. 2020

Advances in Polymer Technology

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Cadmium-tolerant (6 mM) Aspergillus niger (RCMB 002002) biomass was challenged with aqueous cadmium chloride (1 mM) followed by sodium sulfide (9 mM) at 37°C for 96 h under shaking conditions (200 rpm), resulting in the formation of highly stable polydispersed cadmium sulfide nanoparticles (CdSNPs). Scanning electron microscopy revealed the presence of spherical particles measuring approximately 5 nm. A light scattering detector (LSD) showed that 100% of the CSNPs measure from 2.7 to 7.5 nm. Structural analyses by both powder X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) confirmed the presence of cubic CdS nanoparticles (CdSNPs) capped with fungal proteins. These CdSNPs showed emission spectra with a broad fluorescence peak at 420 nm and UV absorption onset at 430 nm that shifted to 445 nm after three months of incubation. The CdSNPs showed antimicrobial activity against E. coli, Pseudomonas vulgaris, Staphylococcus aureus, and Bacillus subtilis, and no antimicrobial activity was detected against Candida albicans. The biosynthesized CdSNPs have cytotoxic activity, with 50% inhibitory concentrations (IC50) of 190 μg mL-1 against MCF7, 246 μg mL-1 against PC3, and 149 μg mL-1 against A549 cell lines.

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A role for biosynthetic CdS quantum dots in extracellular electron transfer of Saccharomyces cerevisiae

Cited by 30 publications

References 27 publications

Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Anticancer and Antibacterial Activity of Cadmium Sulfide Nanoparticles byAspergillus niger

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