A role for juvenile hormone in the prepupal development of <i>Drosophila melanogaster</i>

Riddiford, Lynn M.; Truman, James W.; Mirth, Christen K.; Shen, Yannan

doi:10.1242/dev.037218

Cited by 172 publications

(185 citation statements)

References 42 publications

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“…1 A-C). Because the removal of the CA is prepupal lethal (23,24), we used the temperature-sensitive GAL80 (GAL80 ts ) transgene to bypass this critical period for JH during development. At 60 h after puparium formation (APF), we shifted the pupae Significance Juvenile hormone (JH) regulates reproductive maturation in insects.…”

Section: Resultsmentioning

confidence: 99%

“…effects of allatectomy during larval life (23)(24)(25). Therefore, to determine whether gce had any effect on the maturation of mating behavior, we used a null allele of gce (gce 2.5k ), which is a 2.5 kb deletion in the gce locus that causes loss of gce transcripts (25).…”

Section: Resultsmentioning

confidence: 99%

“…Met plays a distinct role in adult optic lobe development during metamorphosis, whereas Gce has no effect (24,38). Met is also the predominant receptor required for the effects of JH on ovarian maturation, both the normal timing and normal fecundity (25).…”

Section: Discussionmentioning

confidence: 99%

“…Loss of function of both receptor genes is necessary to mimic the effect of genetic ablation of the CA in larvae, which causes prepupal lethality (23)(24)(25). In the adult, loss of Met causes both delayed and reduced fecundity, whereas loss of gce has only a slight effect on ovarian maturation (25).…”

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confidence: 99%

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Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Bilen

Atallah

Azanchi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Juvenile hormone (JH) coordinates timing of female reproductive maturation in most insects. In Drosophila melanogaster, JH plays roles in both mating and egg maturation. However, very little is known about the molecular pathways associated with mating. Our behavioral analysis of females genetically lacking the corpora allata, the glands that produce JH, showed that they were courted less by males and mated later than control females. Application of the JH mimic, methoprene, to the allatectomized females just after eclosion rescued both the male courtship and the mating delay. Our studies of the null mutants of the JH receptors, Methoprene tolerant (Met) and germ cell-expressed (gce), showed that lack of Met in Met 27 females delayed the onset of mating, whereas lack of Gce had little effect. The Met 27 females were shown to be more attractive but less behaviorally receptive to copulation attempts. The behavioral but not the attractiveness phenotype was rescued by the Met genomic transgene. Analysis of the female cuticular hydrocarbon profiles showed that corpora allata ablation caused a delay in production of the major female-specific sex pheromones (the 7,11-C27 and -C29 dienes) and a change in the cuticular hydrocarbon blend. In the Met 27 null mutant, by 48 h, the major C27 diene was greatly increased relative to wild type. In contrast, the gce 2.5k null mutant females were courted similarly to control females despite changes in certain cuticular hydrocarbons. Our findings indicate that JH acts primarily via Met to modulate the timing of onset of female sex pheromone production and mating.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Bilen

Atallah

Azanchi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…It is widely considered that this "status quo" action of JH is required to maintain the larval status throughout the larval stage. Interestingly, however, several attempts to deplete JHs using classic and modern techniques have failed to induce precocious metamorphosis during the very early larval instars (6)(7)(8)(9)(10)(11)(12).…”

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confidence: 99%

Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle

Daimon

Uchibori

Nakao

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

133

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Insect juvenile hormones (JHs) prevent precocious metamorphosis and allow larvae to undergo multiple rounds of status quo molts. However, the roles of JHs during the embryonic and very early larval stages have not been fully understood. We generated and characterized knockout silkworms (Bombyx mori) with null mutations in JH biosynthesis or JH receptor genes using genome-editing tools. We found that embryonic growth and morphogenesis are largely independent of JHs in Bombyx and that, even in the absence of JHs or JH signaling, pupal characters are not formed in first-or second-instar larvae, and precocious metamorphosis is induced after the second instar at the earliest. We also show by mosaic analysis that a pupal specifier gene broad, which is dramatically up-regulated in the late stage of the last larval instar, is essential for pupal commitment in the epidermis. Importantly, the mRNA expression level of broad, which is thought to be repressed by JHs, remained at very low basal levels during the early larval instars of JH-deficient or JH signaling-deficient knockouts. Therefore, our study suggests that the long-accepted paradigm that JHs maintain the juvenile status throughout larval life should be revised because the larval status can be maintained by a JHindependent mechanism in very early larval instars. We propose that the lack of competence for metamorphosis during the early larval stages may result from the absence of an unidentified broadinducing factor, i.e., a competence factor. juvenile hormone | metamorphosis | ecdysone | TALEN | Bombyx

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Potential targets for controlling Bactrocera dorsalis using cuticle‐ and hormone‐related genes revealed by a developmental transcriptome analysis

Liu

Xia

Zhang

et al. 2020

Pest Management Science

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BACKGROUND: The oriental fruit fly, Bactrocera dorsalis (Hendel), is an important agricultural pest and has developed resistance to many insecticides. To investigate vital genes participating in metamorphosis for development of additional control strategies, a comprehensive transcriptome analysis covering ten developmental stages of B. dorsalis was performed.RESULTS: There were 2132, 952, 1062, 2301 and 1333 differentially expressed genes identified during hatching, 1st-instar larval molting, 2nd-instar larval molting, pupariation and emergence, respectively. Further expression analyses indicated that genes in hormone-(20-hydroxyecdysone and juvenile hormone) and cuticle-(chitin and cuticle protein) related pathways were essential for metamorphosis in B. dorsalis. Among chitinase (Cht) genes, BdCht-5, −8 and −10 were differentially expressed during larval-larval, larval-pupal and pupal-adult moltings. However, BdCht7 was differentially expressed during egg-larval and larval-larval moltings. Knockdown of BdCht7 at the 1st-instar larval stage disrupted normal development of larvae and was lethal to B. dorsalis. Among cuticle protein (CP) genes, 15 genes (BdCPLCG-1, and BdRR1-41) were differentially expressed during four of five types of moltings. Among hormone-relative genes, BdJHBP-4, −9 and −13 were differentially expressed during all five types of moltings, whereas BdJHBP-5, −12 and BdHR4 were differentially expressed during four of five types of moltings.CONCLUSION: This study reveals critical genes involved in development and metamorphosis of B. dorsaslis, and BdCht7 is dispensable for larval survival. It also provides comprehensive transcriptome information for finding more molecular targets to control this pest.

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A role for juvenile hormone in the prepupal development of Drosophila melanogaster

Cited by 172 publications

References 42 publications

Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle

Potential targets for controlling Bactrocera dorsalis using cuticle‐ and hormone‐related genes revealed by a developmental transcriptome analysis

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