A role for macrophage migration inhibitory factor in protective immunity against Aspergillus fumigatus

Stojanović, Ivana; Mirkov, Ivana; Kataranovski, Milena; Glamočlija, Jasmina; Stošić‐Grujičić, Stanislava

doi:10.1016/j.imbio.2011.03.005

Cited by 25 publications

(17 citation statements)

References 74 publications

(89 reference statements)

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“…Macrophages are essential to the innate immunity and pathology of tissue injury and inflammation [27] through phagocytosis. Macrophages secrete cytokines, such as interleukins, TNF-α, and INFs, as well as inflammatory mediators, such as nitric oxide [28]. Macrophages maintain homeostasis and serve an important function in the host defense against pathogens and attacking cells, such as cancer cells [29].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8

Abood

Fahmi

Abdulla

et al. 2014

BMC Complement Altern Med

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BackgroundImmunomodulators are substances that modify immune system response to a threat. Immunomodulators modulate and potentiate the immune system, keeping it highly prepared for any threat. The immunomodulatory effect of the traditional medicine Tinospora crispa is investigated in this work.MethodsT. crispa ethanol extract was fractionated by using different solvents. The ethanol extract and effective isolated fraction were used to investigate the potential immunomodulatory effect of different T. crispa doses ranging from 25 μg/mL to 1000 μg/mL on RAW 246.7 cells by detecting intracellular INF-γ, IL-6, and IL-8 expressions. The antioxidant activity of T. crispa was evaluated through FRAP and DPPH. The total phenolic and total flavonoid contents were also quantified.ResultsResults show that T. crispa extract has higher antioxidant potential than ascorbic acid. The FRAP value of T. crispa extract is 11011.11 ± 1145.42 μmol Fe+2/g, and its DPPH inhibition percentage is 55.79 ± 7.9, with 22 μg/mL IC50. The results also reveal that the total phenolic content of T. crispa extract is 213.16- ± 1.31 mg GAE/g dry stem weight, and the total flavonoid content is 62.07- ± 39.76 mg QE/g dry stem weight. T. crispa crude extract and its isolated fraction significantly stimulate RAW264.7 cell viability (P ≤ 0.05) and intracellular INF-γ, IL-6, and IL-8 expressions. The results of LC-MS show that four of the active compounds detected in the T. crispa isolated fraction are cordioside, quercetin, eicosenoic acid (paullinic acid), and boldine.ConclusionsThe results of this study obviously indicate that T. crispa has immunomodulatory effects through the stimulation of INF-γ, IL-6, and IL-8 expressions. LC-MS phytochemical analysis showed that the T. crispa fraction has cordioside, quercetin, eicosenoic acid (paullinic acid), and boldine, which may be responsible for the immunostimulator effect of T. crispa.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8

Abood

Fahmi

Abdulla

et al. 2014

BMC Complement Altern Med

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“…Other immunosuppressive medicines were used, like 5-fluorouracil (Hata et al, 1996a,b, 2011; Wallace et al, 1997; Graybill et al, 1998, 2003a; BitMansour et al, 2005; Stojanovic et al, 2011; Salas et al, 2013), tacrolimus (FK506; High and Washburn, 1997; Herbst et al, 2013, 2015; Shirkhani et al, 2015), cyclosporine A (Polak-Wyss, 1991; High and Washburn, 1997), mitotic poisons (Baisakh et al, 1975; Balloy et al, 2005a,b; Loussert et al, 2010; Hein et al, 2015). Tunicamycin was employed once because of its anti-Natural Killer (NK) cell property (Maheshwari et al, 1988), and liposomal dichloromethylene diphosphonate (DMPD) because it decreases macrophages in liver and spleen (Moonis et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

Rodent Models of Invasive Aspergillosis due to Aspergillus fumigatus: Still a Long Path toward Standardization

Désoubeaux

Cray

2017

Front. Microbiol.

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Invasive aspergillosis has been studied in laboratory by the means of plethora of distinct animal models. They were developed to address pathophysiology, therapy, diagnosis, or miscellaneous other concerns associated. However, there are great discrepancies regarding all the experimental variables of animal models, and a thorough focus on them is needed. This systematic review completed a comprehensive bibliographic analysis specifically-based on the technical features of rodent models infected with Aspergillus fumigatus. Out the 800 articles reviewed, it was shown that mice remained the preferred model (85.8% of the referenced reports), above rats (10.8%), and guinea pigs (3.8%). Three quarters of the models involved immunocompromised status, mainly by steroids (44.4%) and/or alkylating drugs (42.9%), but only 27.7% were reported to receive antibiotic prophylaxis to prevent from bacterial infection. Injection of spores (30.0%) and inhalation/deposition into respiratory airways (66.9%) were the most used routes for experimental inoculation. Overall, more than 230 distinct A. fumigatus strains were used in models. Of all the published studies, 18.4% did not mention usage of any diagnostic tool, like histopathology or mycological culture, to control correct implementation of the disease and to measure outcome. In light of these findings, a consensus discussion should be engaged to establish a minimum standardization, although this may not be consistently suitable for addressing all the specific aspects of invasive aspergillosis.

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“…Previous studies investigating these cytokines and chemokines have provided critical insight into their role in lung defence against A. fumigatus 1,19 . IL‐12 and IL‐2 can enhance the IFN‐γ expression in the pulmonary tissue, reduce the colony load in the pulmonary tissue and increase the survival rate of mouse, compared with saline solution control group.…”

Section: Discussionmentioning

confidence: 99%

“…Epidemiological issues of fungal infection, mainly caused by Candida species and Aspergillus fumigatus , are dramatically increasing in the past decade 1 . Invasive pulmonery aspergillosis (IPA) is a leading cause of mortality in solid organ transplant recipients for haematological malignancies and aplastic anaemia patients 1 . The immunosuppressants used to maintain transplanted organ grafts often increase susceptibility to this infection.…”

Section: Introductionmentioning

confidence: 99%

Interleukin‐12 and interleukin‐2 alone or in combination against the infection in invasive pulmonary aspergillosis mouse model

Zhang

Lin

et al. 2012

Mycoses

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Summary Aspergillus fumigatus is an intracellular opportunistic fungus causing invasive pulmonary mycosis, characterised by hyphal invasion and destruction of pulmonary tissue. Th1 cytokines could enhance fungicidal activity. The effects from the combination of interleukin‐12 (IL‐12) and IL‐2 are rarely known in invasive pulmonary aspergillosis infection. To assess the cleaning of A. fumigatus infection in the pulmonary tissues by IL‐12 and IL‐2, interferon‐γ (IFN‐γ) was detected in the sera using ELISA, quantification of IFN‐γ mRNA using real‐time RT‐PCR and lung Colony‐forming unit was assayed by cultivation. Morphology was analysed by histopathological examination. Our results showed that IL‐12 and/or IL‐2 could enhance the IFN‐γ expression in the pulmonary tissue, reduce the colony load in the pulmonary tissue and increase the survival rate of mouse. The combination of IL‐12 and IL‐2 could assist in increasing the IFN‐γ expression in the pulmonary tissue, but neither reduce colony load in the pulmonary tissue nor increase the survival rate of mouse significantly. It was demonstrated that IL‐12 and IL‐2 were strong immunomodulatory cytokines as a prerequisite for protecting the host from infectious agents.

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A role for macrophage migration inhibitory factor in protective immunity against Aspergillus fumigatus

Cited by 25 publications

References 74 publications

Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8

Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8

Rodent Models of Invasive Aspergillosis due to Aspergillus fumigatus: Still a Long Path toward Standardization

Interleukin‐12 and interleukin‐2 alone or in combination against the infection in invasive pulmonary aspergillosis mouse model

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