1996
DOI: 10.1126/science.274.5288.780
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A Role for Phosphoinositide 3-Kinase in Bacterial Invasion

Abstract: Listeria monocytogenes is a bacterial pathogen that invades cultured nonphagocytic cells. Inhibitors and a dominant negative mutation were used to demonstrate that efficient entry requires the phosphoinositide (PI) 3-kinase p85alpha-p110. Infection with L. monocytogenes caused rapid increases in cellular amounts of PI(3, 4)P2 and PI(3,4,5)P3, indicating that invading bacteria stimulated PI 3-kinase activity. This stimulation required the bacterial protein InlB, host cell tyrosine phosphorylation, and associati… Show more

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Cited by 320 publications
(350 citation statements)
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“…As well established (17,18), inhibition of actin even by low concentrations of cytochalasin D or latrunculin B was found to have potent negative effects on bacterial invasion of HeLa cells (supplemental Fig. S5A).…”
Section: Discussionmentioning
confidence: 98%
See 1 more Smart Citation
“…As well established (17,18), inhibition of actin even by low concentrations of cytochalasin D or latrunculin B was found to have potent negative effects on bacterial invasion of HeLa cells (supplemental Fig. S5A).…”
Section: Discussionmentioning
confidence: 98%
“…Survival assays involving drug treatments were performed as described for other Listeria in vitro systems (17,18), where cells were treated with various concentrations of drugs 30 min prior to infection. Upon infection, host cells were incubated in drugs (at molar concentrations varying from 200 to 0.01 M) throughout the gentamicin survival assays as detailed above.…”
Section: Methodsmentioning
confidence: 99%
“…1B). Stimulation by InlB triggers the classical phosphoinositide (PI) 3-kinase pathway [Ireton et al, 1996], via recruitment of Gab1, Cbl, and Shc on phosphotyrosine residues of the intracytoplasmic portion of Met. How this pathway then activates the small G proteins Rac and Cdc42 is currently unknown, although the organization of the membrane in micro-domains is critical for Rac activation probably by controlling the distribution of 3 0 -PIs generated by PI 3-kinase [Seveau et al, 2007].…”
Section: The Inlb/met Pathway Of Listeria Entrymentioning
confidence: 99%
“…81,82 Although InlB has several receptors, it is well accepted that Met is the major InlB signaling receptor. InlB has the ability to induce Met autophosphorylation and the recruitment of adaptor proteins, such as Cbl, Shc and Gab1, [83][84][85] that lead to the activation of PI3-kinase 86 and small GTPase Rac1. 87 Upon interaction with Met, InlB induces Met ubiquitination and bacterial internalization via a clathrin-mediated endocytosis mechanism.…”
Section: O N O T D I S T R I B U T Ementioning
confidence: 99%