2000
DOI: 10.1095/biolreprod62.6.1647
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A Role for Phosphorylation of Glycogen Synthase Kinase-3α in Bovine Sperm Motility Regulation1

Abstract: The long-term goal of our work is to understand biochemical mechanisms underlying sperm motility and fertility. In a recent study we showed that tyrosine phosphorylation of a 55-kDa protein varied in direct proportion to motility. Tyrosine phosphorylation of the protein was low in immotile compared to motile epididymal sperm. Inhibition or stimulation of motility by high calcium levels or cAMP, respectively, results in a corresponding decrease or increase in tyrosine phosphorylation of the 55-kDa protein. Here… Show more

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Cited by 75 publications
(66 citation statements)
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“…Moreover, these authors describe the presence of both the a and b GSK3 isoforms in bovine epididymal spermatozoa (Vijayaraghavan et al , 1997 and discovered a 55 kDa protein whose tyrosine phosphorylation was closely correlated to the motility state of epididymal bovine spermatozoa and the treatment of these cells with motility activators, like isobutylmethylxanthine or 8Br-cAMP, resulted in increased tyrosine phosphorylation of this protein (Vijayaraghavan et al 1997). The same authors showed a correlation between the 55 kDa tyrosine phosphorylation motility-associated protein and the GSK3 and suggested that GSK3, which is also regulated by tyrosine phosphorylation, could be an important key underlying motility initiation in the epididymis (Vijayaraghavan et al 2000). Finally, Somanath et al (2004) showed that serine phosphorylation of GSK3 increases significantly in bovine spermatozoa during their passage through the epididymis with a parallel decrease in GSK3 activity (Somanath et al 2004).…”
Section: Introductionmentioning
confidence: 94%
“…Moreover, these authors describe the presence of both the a and b GSK3 isoforms in bovine epididymal spermatozoa (Vijayaraghavan et al , 1997 and discovered a 55 kDa protein whose tyrosine phosphorylation was closely correlated to the motility state of epididymal bovine spermatozoa and the treatment of these cells with motility activators, like isobutylmethylxanthine or 8Br-cAMP, resulted in increased tyrosine phosphorylation of this protein (Vijayaraghavan et al 1997). The same authors showed a correlation between the 55 kDa tyrosine phosphorylation motility-associated protein and the GSK3 and suggested that GSK3, which is also regulated by tyrosine phosphorylation, could be an important key underlying motility initiation in the epididymis (Vijayaraghavan et al 2000). Finally, Somanath et al (2004) showed that serine phosphorylation of GSK3 increases significantly in bovine spermatozoa during their passage through the epididymis with a parallel decrease in GSK3 activity (Somanath et al 2004).…”
Section: Introductionmentioning
confidence: 94%
“…2). Some specific protein targets of tyrosine phosphorylation in sperm have been suggested and phosphorylation of these proteins has been closely linked to the onset of motility in bovine sperm (Vijayaraghavan et al 1997b(Vijayaraghavan et al , 2000.…”
Section: Motilitymentioning
confidence: 99%
“…In addition, there is increasing evidence suggesting a role for different kinases and phosphatases regulating sperm motility (Tash & Bracho 1994, Vijayaraghavan et al 2000, Luconi et al 2001, NagDas et al 2002. Recently, the presence of the phosphatidylinositol 3-kinase (PI3-K) has been demonstrated in mouse, hamster and human spermatozoa (Feng et al 1998, NagDas et al 2002.…”
Section: Introductionmentioning
confidence: 99%