A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function

Lang, Patricia; Christie, Michael; Dogan, Ezgi Sueheyla; Schwab, Rebecca; Hagmann, Joerg; Weyer, Anna-Lena Van de; Scacchi, Emanuele; Weigel, Detlef

doi:10.1104/pp.17.01313

Cited by 28 publications

(40 citation statements)

References 80 publications

(143 reference statements)

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“…Failed degradation of non‐optimal RISC would likely increase the pool of corresponding normal RISC through RISC recycling (Figure E) (Jo et al ). This model does not contradict the results from Lang et al (), although they favored a role of HWS upstream of miRNA action.…”

supporting

confidence: 63%

“…Nevertheless, small RNA‐seq analysis revealed that only a few miRNAs, including miR164, miR168, miR319 and miR398, were upregulated in the hws‐6 mutant and/or down‐regulated in the p35S :: HWS plants, which were subsequently validated by Northern blot (Figure G–I, S4 and Table S3). These results suggested that HWS may only affect a subset of miRNAs, although we cannot exclude the possibility that it may have broader functions with respect to the miRNA pathway (Lang et al ).…”

mentioning

confidence: 86%

“…Interestingly, HWS has recently been recovered from an independent screen system based on the miRNA target mimicry (MIM) system (Lang et al ). Thus, HWS is critical for efficient block of different miRNA functions in different target mimicry systems.…”

mentioning

confidence: 99%

“…Since HWS dysfunction also suppresses multiple MIM induced developmental defects (Lang et al ), it is likely that the effect of HWS is magnified in the presence of mimicry targets. This is in agreement with only minor developmental defects observed in hws mutant, as a general over‐accumulation of miRNAs would result in drastic developmental abnormalities (Ramachandran and Chen ).…”

mentioning

confidence: 99%

“…F‐box proteins provide E3 ubiquitin ligases with target specificity via recognition of substrates for ubiquitination followed by degradation through the ubiquitin proteasome system or selective autophagy pathways (Gonzalez‐Carranza et al ; Kraft et al ). However, yeast two hybrid and Western blot assays suggested that HWS may neither interact with the core miRNA biogenesis pathway proteins nor affect their expression levels (Figure S2) (Lang et al ). It is possible that an unknown protein(s) may bridge HWS‐mediated miRNA regulation, or that HWS‐mediated substrate recognition and degradation require specific conformational changes and/or post‐translational modifications.…”

mentioning

confidence: 99%

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The F‐box protein HAWAIIAN SKIRT is required for mimicry target‐induced microRNA degradation in Arabidopsis

Mei

Jiang

Ren

2019

JIPB

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Summary Mimicry target‐directed microRNA degradation is widespread and highly conserved among eukaryotes. However, little is known about its mechanism of action. In this letter, by using STTM160 (target mimic of miR160) as a reporter, we show that dysfunction of HAWAIIAN SKIRT (HWS) suppresses the pleiotropic phenotype of STTM160. Small RNA sequencing and Northern blot analyses suggested that HWS only affects a subset of microRNAs. Intriguingly, we identified a stable coexistence of miR160/miR399 and their mimicry targets within the AGO1 complex when HWS is compromised, pointing to a possible role of HWS in the clearance of RNA‐induced silencing complexes associated with mimicry target.

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supporting

confidence: 63%

mentioning

confidence: 86%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The F‐box protein HAWAIIAN SKIRT is required for mimicry target‐induced microRNA degradation in Arabidopsis

Mei

Jiang

Ren

2019

JIPB

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miRNAs in Plant Development

Swarup

Denyer

2019

Annual Plant Reviews Online

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Since the discovery of the first microRNA (miRNA) in Caenorhabditis elegans about 25 years ago, small RNAs have emerged as key players in the broad regulation of gene expression in most developmental processes in both plants and animals. miRNAs are small (20–24 nt) noncoding RNA molecules involved in post‐transcriptional regulation of gene expression. miRNAs regulate gene expression by cleavage of the target transcript and/or through translational repression. The former appears to be a more prevalent mechanism in plants whereas the later appears to be predominant in animals. The level of miRNA‐target sequence complementarity typically dictates whether mRNA repression or transcript cleavage occurs. This article will focus on our current understanding of the roles of miRNAs in plant development with a key focus on developmentally important miRNAs of the model plant, Arabidopsis thaliana , their modes of action, and the networks to which they belong. This article will also briefly discuss conservation of microRNAs across other plant species and directions for future research on miRNA.

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Fraternity of old‐timers: How ubiquitin regulates miRNA functions

Ryazansky

Akulenko

2023

BioEssays

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miRNA‐mediated gene repression and ubiquitin‐dependent processes are among the oldest and most versatile mechanisms that control multiple molecular pathways, rather than just protein turnover. These systems were discovered decades ago and have become among the most studied. All systems within cells are interconnected, and these two are no exception: the plethora of studies have demonstrated that the activity of the miRNAs system depends on players of the ubiquitin‐centered universe of processes, and vice versa. This review focuses on recent progress that highlights that very similar mechanisms of regulation of miRNAs by ubiquitin‐related processes are likely to be found in distantly related species, including animals, plants, and viruses. Most of them occur through the ubiquitination of Argonaute proteins, but some of the other miRNA system factors are also regulated. This suggests that their regulatory relationships are either ancient evolutionary acquisitions or have arisen independently in different kingdoms.

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A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function

Cited by 28 publications

References 80 publications

The F‐box protein HAWAIIAN SKIRT is required for mimicry target‐induced microRNA degradation in Arabidopsis

The F‐box protein HAWAIIAN SKIRT is required for mimicry target‐induced microRNA degradation in Arabidopsis

miRNAs in Plant Development

Fraternity of old‐timers: How ubiquitin regulates miRNA functions

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