A role for the tetraspanin proteins in Salmonella infection of human macrophages

Hassuna, Noha A.; Monk, Peter N.; Ali, Fawwaz; Read, Robert C.; Partridge, Lynda J.

doi:10.1016/j.jinf.2017.06.003

Cited by 10 publications

(15 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further study is required to investigate the role of CD9 as well as other tetraspanins for B. pseudomallei internalisation and to identify their partner proteins for the organisation of "entry platforms" for B. pseudomallei infection. Our data cannot suggest that overexpression of CD9 in A549 leads to a decrease in internalisation during B. pseudomallei infection but data from Hassuna NA et al suggest that overexpression of CD63 in HEK 293 cells is not sufficient to promote S. Typhimurium binding to non-phagocytic cells 30 .…”

Section: Discussioncontrasting

confidence: 71%

“…There is also growing evidence for roles for tetraspanins in bacterial infection; for example, CD81 has been demonstrated to be involved in Listeria monocygenes invasion into human epithelial cells 28 . TEMs appear to act as "adhesion platforms" for a wide range of bacteria, and that agents that disrupt TEM can prevent bacterial attachment to host cells [29][30][31] . B. pseudomallei employs several virulence factors for adhesion and invasion to host cells such as PilA and adhesion proteins; BoaA and BoaB 32 .…”

Section: Discussionmentioning

confidence: 99%

“…MAbs that recognised mouse tetraspanins, including MAbs specific to CD9 (clone MF1, Bio-Rad), CD63 (clone NVG-2, BioLegend Inc., San Diego, CA, USA), CD81 (clone Eat2, Bio-Rad), and rat IgG2b, rat IgG2a, and hamster IgG1 isotype controls were used to treat J774A.1 cells. The production of recombinant glutathione S-transferase (GST) fusion proteins with CD9, CD63, and CD81 tetraspanin EC2 extracellular domains were previously described 30 .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

Sangsri

Saiprom

Tubsuwan

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Tetraspanins are four-span transmembrane proteins of host cells that facilitate infections by many pathogens. Burkholderia pseudomallei is an intracellular bacterium and the causative agent of melioidosis, a severe disease in tropical regions. This study investigated the role of tetraspanins in B. pseudomallei infection. We used flow cytometry to determine tetraspanins CD9, CD63, and CD81 expression on A549 and J774A.1 cells. Their roles in B. pseudomallei infection were investigated in vitro using monoclonal antibodies (MAbs) and recombinant large extracellular loop (EC2) proteins to pretreat cells before infection. Knockout of CD9 and CD81 in cells was performed using CRISPR Cas9 to confirm the role of tetraspanins. Pretreatment of A549 cells with MAb against CD9 and CD9-EC2 significantly enhanced B. pseudomallei internalization, but MAb against CD81 and CD81-EC2 inhibited MNGC formation. Reduction of MNGC formation was consistently observed in J774.A1 cells pretreated with MAbs specific to CD9 and CD81 and with CD9-EC2 and CD81-EC2. Data from knockout experiments confirmed that CD9 enhanced bacterial internalization and that CD81 inhibited MNGC formation. Our data indicate that tetraspanins are host cellular factors that mediated internalization and membrane fusion during B. pseudomallei infection. Tetraspanins may be the potential therapeutic targets for melioidosis.

show abstract

Section: Discussioncontrasting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

Sangsri

Saiprom

Tubsuwan

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the current study, we determined that CD22 -like abundantly expressed in resting and activated macrophages. In mammals, it has been shown that tetraspanins were expressed differentially in monocyte subsets, with CD63 exhibiting higher expression level; what is more, CD63 could bind with typhimurium, which confirmed its importance [ 49 ]. We speculate that CD63 also plays an important role in bacterial infection in C. idella .…”

Section: Discussionmentioning

confidence: 98%

Transcriptome Analysis Provides Insights into the Markers of Resting and LPS-Activated Macrophages in Grass Carp (Ctenopharyngodon idella)

Wei

Liao

et al. 2018

IJMS

View full text Add to dashboard Cite

Macrophages are very versatile immune cells, with the characteristics of a proinflammatory phenotype in response to pathogen-associated molecular patterns. However, the specific activation marker genes of macrophages have not been systematically investigated in teleosts. In this work, leukocytes (WBC) were isolated using the Percoll gradient method. Macrophages were enriched by the adherent culture of WBC, then stimulated with lipopolysaccharide (LPS). Macrophages were identified by morphological features, functional activity and authorized cytokine expression. Subsequently, we collected samples, constructed and sequenced transcriptomic libraries including WBC, resting macrophage (Mø) and activated macrophage (M(LPS)) groups. We gained a total of 20.36 Gb of clean data including 149.24 million reads with an average length of 146 bp. Transcriptome analysis showed 708 differential genes between WBC and Mø, 83 differentially expressed genes between Mø and M(LPS). Combined with RT-qPCR, we proposed that four novel cell surface marker genes (CD22-like, CD63, CD48 and CD276) and two chemokines (CXCL-like and CCL39.3) would be emerging potential marker genes of macrophage in grass carp. Furthermore, CD69, CD180, CD27, XCL32a.2 and CXCL8a genes can be used as marker genes to confirm whether macrophages are activated. Transcriptome profiling reveals novel molecules associated with macrophages in C. Idella, which may represent a potential target for macrophages activation.

show abstract

“…While recombinant proteins reduced binding of S. typhimurium to macrophages by 50%, monoclonal antibodies showed varying results, with some enhancing (CD37) and others inhibiting (CD63 and CD81) adhesion. Knockdown of CD63 by siRNA treatment or using monoclonal antibodies against CD63 greatly reduced bacterial adhesion to macrophages, emphasising the importance of CD63 in Salmonella pathogenesis (Figure 2F) (Hassuna, Monk, Ali, Read, & Partridge, 2017).…”

Section: Tetraspanin‐dependent Invasion and Colonisation Of Host Cellmentioning

confidence: 89%

The roles of tetraspanins in bacterial infections

Karam

Méresse

Kremer

et al. 2020

Cellular Microbiology

View full text Add to dashboard Cite

Tetraspanins, a wide family composed of 33 transmembrane proteins, are associated with different types of proteins through which they arbitrate important cellular processes such as fusion, adhesion, invasion, tissue differentiation and immunological responses. Tetraspanins share a comparable structural design, which consists of four hydrophobic transmembrane domains with cytoplasmic and extracellular loops. They cooperate with different proteins, including other tetraspanins, receptors or signalling proteins to compose functional complexes at the cell surface, designated tetraspanin‐enriched microdomains (TEM). Increasing evidences establish that tetraspanins are exploited by numerous intracellular pathogens as a doorway for entering and replicating within human cells. Although previous surveys focused mainly on viruses and parasites, it is now becoming clear that bacteria interact with tetraspanins, using TEM as a “gateway” to infection. In this review, we examine the biological functions of tetraspanins that are relevant to bacterial infective procedures and consider the available data that reveal how different bacteria benefit from host cell tetraspanins in infection and in the pathogenesis of diseases. We will also emphasise the stimulating potentials of targeting tetraspanins for preventing bacterial infectious diseases, using specific neutralising antibodies or anti‐adhesion peptide‐based therapies. Such innovative therapeutic opportunities may deliver alternatives for fighting difficult‐to‐manage and drug‐resistant bacterial pathogens.

show abstract

A role for the tetraspanin proteins in Salmonella infection of human macrophages

Cited by 10 publications

References 47 publications

Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

Transcriptome Analysis Provides Insights into the Markers of Resting and LPS-Activated Macrophages in Grass Carp (Ctenopharyngodon idella)

The roles of tetraspanins in bacterial infections

Contact Info

Product

Resources

About