A Role of Cyclic AMP in the Gastrointestinal Tract: Receptor Control of Hydrogen Ion Secretion by Mammalian Gastric Mucosa

Thompson, W. J.; Jacobson, Eugene D.; Rosenfeld, Gary C.

doi:10.1007/978-3-642-68393-0_16

Cited by 6 publications

(5 citation statements)

References 134 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GU, and BII patients. The site of the histamine-sensitive enzyme is the parietal cell [1,11,13], and the distinctly shaped concentration-response curves un doubtedly reflect the different parietal cell concentrations as found in the preceding study. Thus, the number of parietal cells per biopsy and assay tube determine the degree of activation.…”

Section: Discussionmentioning

confidence: 82%

“…As with histamine, PGE2 stimulates another gastric mucosal AC-cAMP system which is not lo calized in the parietal cells, and its function is still unknown. From studies with isolated cells of rat [1,8,9], dog [10], and investiga tions in human gastric mucosa [11][12][13], it is now known that the PGE2-sensitive enzyme originates from nonparietal cells. Thus, the present data are interpreted according to the number of parietal and nonparietal cells, as evaluated in the preceeding paper.…”

Section: Discussionmentioning

confidence: 99%

“…Histamine binds to H2 receptors on the surface of the parietal cell, and it is generally agreed in most species including man that the ligand receptor complex causes activation of the AC-cAMP system which is part of the mechanism stimulating H+ secretion [1], al though recently partly contradictory results have been reported in guinea pigs [7]. As with histamine, PGE2 stimulates another gastric mucosal AC-cAMP system which is not lo calized in the parietal cells, and its function is still unknown.…”

Section: Discussionmentioning

confidence: 99%

“…There is evidence that the histamine H2-receptor-sensitive AC is involved in the process of H+ secretion [re view in ref. 1], whereas the role of the prosta glandin E? (PGE2)-sensitivc enzyme is un known, e.g.. if it is connected with cytoprotection [review in ref.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

Leyhe

et al. 1986

Pharmacology

View full text Add to dashboard Cite

Adenylate cyclase (AC) in response to prostaglandin E₂ (PGE₂) and histamine was studied in morphologically different biopsy specimens from human gastric mucosa. The activities of the enzyme were log-normally distributed and did not differ between males and females. PGE₂ activated AC in a concentration-dependent manner in normal gastric mucosa (n = 57), chronic superficial gastritis (GI, 18), chronic gastritis with beginning atrophy (GII, 10), chronic atrophic gastritis (GUI, 24), gastric ulcer (GU, 39), duodenal ulcer (DU, 32), and biopsies of patients operated according to Billroth II (BII, 20) and was most efficacious in GUI and BII. Histamine, which was studied in normal gastric mucosa (n = 27), DU (n = 20), GU (n= 13), and BII (n = 18), stimulated AC most efficaciously and potently in DU, was less effective in normal gastric mucosa and GU, and had no effect at all in BII. Cimetidine treatment of DU patients did not change the PGE₂ action, while the degree of stimulation by histamine was reduced. The data indicate characteristic differences of the PGE₂- and histamine-sensitive AC in the mucosal samples of these patients.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

Leyhe

et al. 1986

Pharmacology

View full text Add to dashboard Cite

show abstract

“…Activation of adenylate cyclase is supposed to be an essential intraceUular step in parietal cell H + production [20]. We investigated indirectly by dbcAMP if the same is valid for IF secretion from rat chief cells.…”

Section: Discussionmentioning

confidence: 99%

Intrinsic factor secretion from isolated gastric mucosal cells of rat and man — two different patterns of secretagogue control

Schepp¹,

Miederer²,

Ruoff

1984

Agents and Actions

View full text Add to dashboard Cite

Intrinsic Factor (IF) secretion was studied using isolated gastric mucosal cells from rat and man. In the rat, IF was localized to the chief cells and its secretion responded most efficaciously to carbachol. DbcAMP and hexoprenaline were less powerful, whereas histamine and pentagastrin lacked any effect. In man, IF secretion derived from the parietal cells and was increasingly enhanced by hexoprenaline, pentagastrin, carbachol, histamine and dbcAMP. In both species, IF secretion differs with respect to its cellular origin and the pattern of secretagogue control: IF release from rat chief cells is due to muscarinic receptor excitation, whereas IF release from human parietal cells responds predominantly to histamine-H2-receptor activation and seems to be mediated by the cAMP system.

show abstract

Fura 2 analysis of cytosolic calcium regulation in elutriated rat gastric parietal cells

Black

Cornwell

Lincoln

et al. 1989

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

The calcium probe, Fura 2, is used to establish and partially characterize histamine-, carbachol-, and forskolin-induced calcium transients in enriched parietal cell populations prepared by centrifugal elutriation of dispersed rat fundic mucosa cell isolates. The magnitude of the maximal carbachol response, which is blocked by atropine but not cimetidine, is nearly five times that of histamine or forskolin. Time to peak responses for carbachol, forskolin, and histamine are approximately 7, 17, and 28 sec, respectively. Carbachol-, histamine-, and forskolin-induced increases in Fura 2 fluorescence appear dependent upon extracellular calcium, since these responses are attenuated in low calcium media and blocked by EGTA in low-calcium media or by lanthanum in high- or low-calcium medium. Trifluoperazine and fenoctimine, at concentrations that inhibit secretion, have no effect on either carbachol- or histamine-induced increases in cytosolic calcium. Seven major calcium/EGTA-sensitive phosphoproteins are identified by SDS-PAGE electrophoresis of ATP 32P-labeled cell sonicates. We conclude that cytosolic calcium in enriched rat gastric parietal cell populations is regulated by secretagogue receptor-controlled calcium channels. We postulate that these channels may be controlled by cyclic AMP-dependent phosphorylation, since neither changes in cyclic AMP nor calcium alone mediate the effects of secretagogues entirely, but the interplay between these two second-messenger systems potentiates the actions of these agents. The role of cytosolic calcium as a second messenger in secretagogue action appears similar to that of cyclic AMP in that a specific cellular concentration must be reached to initiate acid secretion.

show abstract

A Role of Cyclic AMP in the Gastrointestinal Tract: Receptor Control of Hydrogen Ion Secretion by Mammalian Gastric Mucosa

Cited by 6 publications

References 134 publications

Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

Intrinsic factor secretion from isolated gastric mucosal cells of rat and man — two different patterns of secretagogue control

Fura 2 analysis of cytosolic calcium regulation in elutriated rat gastric parietal cells

Contact Info

Product

Resources

About