A ROLE OF PROTEINS OF THE OUTER MEMBRANE OF Shewanella oneidensis MR-1 BACTERIA IN FORMATION AND STABILIZATION OF SILVER SULFIDE NANOPARTICLES

Воейкова, А.; Shebanova, S. T.; Ivanov, Danila; Кайшева, Л.; Новикова, М. А.; Zhuravleva, A. I.; Шумянцева, В. В.; Шайтан, В.; Kirpichnikov, P. A.; Дебабов, Г.

doi:10.21519/0234-2758-2015-5-41-48

Cited by 3 publications

(1 citation statement)

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“…[27] However, MR-1 can survive in environments containing a certain concentration of Cu(II) and Ag(I), and produce corresponding NPs. [30,34,36] The growth of MR-1 cultured in 30 mM 3-(N-morpholino)propanesulfonic acid buffer containing 0.01 mM Cu(II) was 100 % compared to the buffer without Cu(II), while the growth decreased to 20 % when Cu(II) increased to 0.10 mM. [30] The resistance of MR-1 to Cu(II) and Ag (I) may derive from the EET, which can be a defense mechanism.…”

Section: Concurrent Reduction Of Cu and Pd By Mr-1mentioning

confidence: 99%

Effect of Copper and Phosphate on the Biosynthesis of Palladium Nanoparticles by Shewanella oneidensis MR‐1

et al. 2020

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The biosynthesis of metallic nanomaterials, especially noble metal nanomaterials, has attracted extensive attention due to the multifarious potential applications of these materials. In this study we have explored the effects of Cu(II) on Pd(II) removal, and of Cu(II) as well as phosphate on the morphologies and composition of biogenic nanoparticles (NPs) from aqueous Pd (II) systems by Shewanella oneidensis MR-1 (MR-1). Cu(II) causes a slight efficiency decrease of Pd(II) removal by MR-1, but a high removal efficiency of 89.8 % is still achieved, which contrasts sharply with the significant Cu(II) inhibition to other microbes. Without phosphate buffer in the medium, 5-nm PdNPs densely envelop MR-1 and accumulate significantly in the periplasm in the absence of Cu(II), while 4-nm PdNPs are evenly distributed in the periplasm, and the cellular surface is smooth in the presence of Cu(II). The following effect of Cu(II) on PdNP synthesis by MR-1 is therefore proposed: Cu(II) significantly affects PdNPs morphologies by inhibiting Pd(0) transfer across the outer membrane and PdNP crystal growth in the periplasm, but not the sorption of Pd(II). Phosphate addition only slightly increases Pd harvest but also precipitates Cu(II) and poses difficulties for the biosynthesis. The study highlights the effect of co-existing metals during the biosynthesis of metallic nanomaterials and offers new insight into the mechanism of PdNP formation and the electron transfer during Pd(II) reduction by MR-1.

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Section: Concurrent Reduction Of Cu and Pd By Mr-1mentioning

confidence: 99%

Effect of Copper and Phosphate on the Biosynthesis of Palladium Nanoparticles by Shewanella oneidensis MR‐1

et al. 2020

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Biotechnological method of obtaining nanoparticles of silver, cadmium and zinc sulfides. Physico-chemical characteristics. Creation of polymeric nanocomposites

Zhuravliova

Воейкова

Bulushova

et al. 2019

Vopr. materialoved.

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A simple and environmentally safe method for obtaining stable nanoparticles of metal sulfides nanoparticles – NpAg2S, NpCdS and NpZnS was developed using different strains of microorganisms in an aqueous solution of metal salts and sulfur sources at the Research Center "Kurchatov Institute" – GosNIIgenetika. The concentration of nanoparticles is 1–4 mg/ml in aqueous suspensions. Using of the methods of electron microscopy, spectrofluorimetry, dynamic light scattering determined the main characteristics of biogenic nanoparticles: shape, size distribution, crystal structure, effective diameter, luminescent spectrum, zeta potential. According to its characteristics, these nanoparticles are referred to quantum dots. It is established that the stability of nanoparticles in aqueous suspensions is due to protein molecules adsorbed on the surface of nanoparticles, which are supplied by cells of microorganisms. Effective immobilization of biogenic nanoparticles on the surface of various polymer supports has been carried out. Biogenic nanoparticles along with nanoparticles obtained by physico-chemical methods can be used as fluorophores for imaging of biological processes, also as photocatalysts, solar cells and for new nanocomposite materials.

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Microbial synthesis and evaluation of bactericidal properties of cadmium sulfide nanoparticles

Журавлева

Воейкова

Кулигин

et al. 2021

Journal of microbiology, epidemiology and immunobiology

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Introduction. The productivity of microbial synthesis of stable nanoparticles is determined by the growth stage of the populations of bacterial cultures used to obtain nanostructures. The study of the biocidal activity of biogenic nanoparticles of cadmium sulfide (NPsCdS), comparable in properties with nanomaterials obtained by physicochemical methods, is promising.The aim of this work was to evaluate the effect of the cell growth phase of the bacterial strains Bacillus subtilis 168 and Shewanella oneidensis MR-1 on the efficiency of biosynthesis of NPsCdS and to study their bactericidal properties against a number of gram-positive and gram-negative strains of microorganisms.Material and methods. Nanoparticles were obtained by introducing Na2 S and CdCl2 salts to a final concentration of 2 mM : 2 mM in liquid bacterial cultures with cells in different phases of growth. The efficiency of NPsCdS biosynthesis was evaluated by the optical density of aqueous nanoparticles solutions. The bactericidal properties of NPsCdS were determined by the diameter of zone of inhibition growth of gram-positive bacteria B. subtilis 168, B. amyloliquefaciens, Streptococcus salivarius, Rhodococcus rhodochrous and gram-negative S. oneidensis MR-1, Escherichia coli K-12, Pseudomonas putida.Results. It was found that the use of cells in the stationary phase of growth (18–24 hours) contributes to obtaining the maximum amount of NPsCdS corresponding to concentrations of 1.0–1.2 mg/ml. The high antimicrobial activity of NPsCdS was shown against gram-positive microorganisms, among gram-negative bacteria, P. putida strain showed insignificant sensitivity.Discussion. The experimental results expand scientific data about the effect of the phase of bacterial growth cycle on biosynthesis of nanoparticles. The stationary phase of growth of B. subtilis 168, S. oneidensis MR-1 is optimal for obtaining of NPsCdS. For the first time, the cytotoxicity of NPsCdS/Shewanella against bacteria of various taxonomic groups was demonstrated. Conclusion. An effective method for obtaining extracellular NPsCdS using bacteria B. subtilis 168, S. oneidensis MR-1 in the stationary phase of growth has been developed. The biocidal activity of biogenic NPsCdS was shown, which allows to consider them as a new class of antimicrobial agents.

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A ROLE OF PROTEINS OF THE OUTER MEMBRANE OF Shewanella oneidensis MR-1 BACTERIA IN FORMATION AND STABILIZATION OF SILVER SULFIDE NANOPARTICLES

Cited by 3 publications

References 0 publications

Effect of Copper and Phosphate on the Biosynthesis of Palladium Nanoparticles by Shewanella oneidensis MR‐1

Effect of Copper and Phosphate on the Biosynthesis of Palladium Nanoparticles by Shewanella oneidensis MR‐1

Biotechnological method of obtaining nanoparticles of silver, cadmium and zinc sulfides. Physico-chemical characteristics. Creation of polymeric nanocomposites

Microbial synthesis and evaluation of bactericidal properties of cadmium sulfide nanoparticles

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