A Routine Diagnostic Test for IgA and IgM Antibodies to Rubella Vims: Absorption of IgG with Staphylococcus aureus

Ankerst, Jaro; Christensen, Poul; Kjellén, Lars; Kronvall, Göran

doi:10.1093/infdis/130.3.268

Cited by 111 publications

(35 citation statements)

References 18 publications

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“…In some patients the IgA antibody response was transient, resembling the IgM antibody response; however, in most of the patients the IgA antibodies persisted at least several months. These results disagree with those obtained by Burgin-Wolff et al (1971) and Cradock-Watson et al (1972), who found the IgA antibodies to persist only 1-2 months after the onset of rash, but strongly support the results of Hornsleth et al (1975) (Ankerst et al 1974) or following removal of serum IgG by precipitation with anti-human-gamma serum (Schmitz et al 1975), a control procedure such as treatment with 2-mercaptoethanol (Roggendorf, Schneweis & Wolff, 1976) should be included.…”

Section: Discussioncontrasting

confidence: 89%

IgA antibody response in acute rubella determined by solid-phase radioimmunoassay

Halonen

Meurman

Matikainen

et al. 1979

J. Hyg.

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SUMMARYA solid-phase radioimmunoassay (RIA) for detecting rubella virus IgA serum antibodies was developed. Purified rubella virus grown in roller cultures of Vero cells was adsorbed onto polystyrene beads. The coated beads were then incubated with dilutions of serum, and rubella JgA antibodies which attached to the virus antigen on the solid-phase were subsequently detected with 125I-labelled antihuman-alpha antibodies. The specificity of the iodinated anti-human immunoglobulins was confirmed by RIA analysis of fractions obtained by chromatography of an early convalescent serum on an agarose column. A complete separation of IgM, IgA, and IgG was observed.A total of 144 serial serum specimens from 31 adult patients with an acute rubella infection were tested for rubella IgA antibodies, and the results were compared with the RIA IgG and IgM titres reported earlier from the same specimens. The RIA IgA response was detected in each of the 31 patients and the IgA antibodies appeared almost simultaneously with the IgG and IgM antibodies. The maximum titres, which were lower than the IgG and IgM titres, were reached in about 1 week after the onset of rash. In 6 patients out of 31 the IgA antibody response was transient and persisted approximately two months, while in the remaining 25 patients the IgA antibodies persisted throughout the study period of more than 5 months. The results obtained indicate that the presence of rubella IgA antibodies in serum is not an indication for a recent rubella infection.

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Section: Discussioncontrasting

confidence: 89%

IgA antibody response in acute rubella determined by solid-phase radioimmunoassay

Halonen

Meurman

Matikainen

et al. 1979

J. Hyg.

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“…The absorption procedure was performed according to the method of Ankerst et al (1). In brief, 20 g of a washed pellet of formalin-killed S. aureus organisms was suspended in 25 ml of the serum diluted 1: 2 with 0.01 M phosphate buffered saline (PBS, pH 7.2) and left at 24 C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, we analyzed the role of the humoral immune factor by adoptive immunization with the antiserum, its immunoglobulin (Ig) M-rich (IgM) and IgG-rich (IgG) fractions, the antiserum absorbed with killed Staphylococcus aureus organisms for reduction of IgG (1), the antiserum absorbed with killed M. pneumoniae organisms, or the antiserum treated with 2-mercaptoethanol (2-ME) for denaturation of IgM.…”

mentioning

confidence: 99%

Role of Humoral Antibodies in Resistance to Mycoplasma pneumoniae Pneumonia in Hamsters

Hayatsu

Kawakubo

Yayoshi

et al. 1980

Microbiology and Immunology

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Golden Syrian hamsters adoptively immunized with hyperimmune Mycoplasma pneumoniae rabbit antiserum, immunoglobulin (Ig) M-rich (IgM) fraction, IgG-rich (IgG) fraction, antiserum absorbed with either killed M. pneumoniae or killed Staphylococcus aureus organisms, or antiserum treated with 2-mercaptoethanol (2-ME) were examined for resistance against aerosol infection with virulent M. pneumoniae. Significant resistance to the establishment of infection in the respiratory tract was shown in hamsters pretreated with the untreated antiserum, IgG fraction or 2-ME-treated antiserum, whereas animals pretreated with the IgM fraction and the antisera absorbed with M. pneumoniae or S. aureus organisms were not significantly resistant. Histopathologically, lung lesions were markedly suppressed in animals with high resistance, but were typically pneumonic in animals with low or no resistance. The efficacy of adoptively administered serum preparations was closely related to their antibody titers. The results indicate that humoral antibody plays an important role in protection against experimental M. pneumoniae pneumonia in hamsters, although the participation of the cell-mediated immune response was not determined.We (8) reported earlier that an inactivated adjuvant vaccine prepared with Mycoplasma pneumoniae strain FH-P24, passaged 24 times in vivo, induced a high level of serum antibody in hamsters and showed a very high protective potency. The results support those of Chanock and his co-workers (2, 11, 13) and McCormick et al (10), who stated that resistance of humans to M. pneumoniae pneumonia was correlated with the presence of naturally acquired or vaccine-induced antibody in serum. In our previous study, also, resistance against M. pneumoniae infection in hamsters corresponded to serum antibody titers, especially those of complement-fixing (CF) antibodies (8).In the present study, we analyzed the role of the humoral immune factor by adoptive immunization with the antiserum, its immunoglobulin (Ig) M-rich (IgM) and IgG-rich (IgG) fractions, the antiserum absorbed with killed Staphylococcus 585

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“…For one experiment serum Stas was absorbed with protein A-containing Staphylococcus aureus strain Cowan I to remove IgG of the subclasses 1, 2 and 4 [2]. 5 ml of a 10% suspension of well washed, formalm-killed S. aureus Cowan I was pelleted, the supernatant discharged and 400 pi of serum Stas diluted 1:3 was added.…”

mentioning

confidence: 99%

A Comparison between Fc Receptors for IgGl and IgG3 on Human Monocytes and Lymphocytes Using Anti-Rh Antibodies

Zupańska¹,

Brojer²,

Maślanka³

et al. 1985

Vox Sanguinis

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In a rosette assay with human erythrocytes equally sensitized with anti-Rh antibodies, the Fc receptor-bearing (FcR) cells are much more numerous among monocytes than among lymphocytes. FcR monocytes could be detected using IgGl and/or IgG3 antibodies although a higher percentage of FcR cells is observed with sera containing IgG3. FcR lymphocytes are detectable almost only with sera containing IgG3 antibodies. If the anti-Rh antibodies are restricted to IgG1 none or single FcR cells could be found. The application of mixed rosette assay with fluorescein-stained erythrocytes sensitized with IgG3 and unstained red cells sensitized with IgGl allowed documentation of the presence of receptors for both subclasses on monocytes as well as on lymphocytes although some differences between these cells were observed. Almost all FcR monocytes bind both IgG subclasses, although in different proportions. On the contrary, most FcR lymphocytes bind only or mainly IgG3, but receptors for IgGl can also be detected.

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A Routine Diagnostic Test for IgA and IgM Antibodies to Rubella Vims: Absorption of IgG with Staphylococcus aureus

Cited by 111 publications

References 18 publications

IgA antibody response in acute rubella determined by solid-phase radioimmunoassay

IgA antibody response in acute rubella determined by solid-phase radioimmunoassay

Role of Humoral Antibodies in Resistance to Mycoplasma pneumoniae Pneumonia in Hamsters

A Comparison between Fc Receptors for IgGl and IgG3 on Human Monocytes and Lymphocytes Using Anti-Rh Antibodies

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