A Saccharomyces cerevisiae-based bioassay for assessing pesticide toxicity

Abstract

This study evaluates the toxic effect of three pesticides (Azoxystrobin, Cymoxanil, and Diuron) on the yeast Saccharomyces cerevisiae for the development of a new bioassay based on inhibition of S. cerevisiae metabolic activity at the level of adenosine-5-triphosphate (ATP) synthesis, as compared with two different toxicity tests based on inhibition of Daphnia magna mobility (NF EN ISO 6341) and inhibition of Vibrio fisheri activity (NF EN ISO 11348). The S. cerevisiae bioassay is cheaper and 96 times faster than the D. magna toxicity bioassay, but has lower sensitivity. It is as fast as the V. fisheri bioassay and more sensitive. Thus, this new toxicity test can be proposed for rapid detection of pesticide residues in environmental samples as a complement to the more expensive and time-consuming D. magna toxicity test.

Photocatalytic degradation and toxicity evaluation of diclofenac by nanotubular titanium dioxide–PES membrane in a static and continuous setup

“…Yeast is a suitable test organism to monitor the toxicity of substances in water with a high sensitivity comparable to standard test systems like Daphnia magna and Vibrio sheri. [27][28][29][30][31] 2. Experimental…”

Influence of microbial and chemical contaminants on the yield and quality of ethanol from wheat grains

“…The significance between all factors analyzed (contamination with F. sporotrichioides , pesticides, and wheat variety) on ethanol yield was found statistically nonsignificant, whereas a significant correlation was found between ethanol yield and wheat samples contamination with pesticides ( r = 0.522; P = 0.01). The toxic effect of pesticides on S. cerevisiae is based on inhibition of yeast metabolic activity at the level of adenosine 5′‐triphosphate synthesis . S. cerevisiae shows affinity for different pesticides, and in particular can partially degrade and significantly adsorb the fungicides .…”

A new biological test of water toxicity–yeast Saccharomyces cerevisiae conductometric test

“…Several papers inform about using of S. cerevisiae to water toxicity testing. For example Esteve et al (2009) presented a bioassay based on inhibition of S. cerevisiae metabolic activity at level of adenosine-5-triphosphate synthesis, as compared with standard toxicity tests with D. magna (EN ISO 6341) and V. fischeri (EN ISO 11348). Three pesticides were tested.…”