A safflower oil based high‐fat/high‐sucrose diet modulates the gut microbiota and liver phospholipid profiles associated with early glucose intolerance in the absence of tissue inflammation

Danneskiold‐Samsøe, Niels Banhos; Andersen, Daniel S.; Radulescu, Ilinca Daria; Normann-Hansen, Ann; Brejnrod, Asker; Kragh, Marie; Madsen, Tobias; Nielsen, Christian; Josefsen, Knud; Fretté, Xavier; Fjære, Even; Madsen, Lise; Hellgren, Lars; Brix, Susanne; Kristiansen, Karsten

doi:10.1002/mnfr.201600528

Cited by 20 publications

(18 citation statements)

References 47 publications

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“…Blautia is a Gram‐positive, anaerobe bacterium belonging to the family Lachnospiraceae , which was thought to take part in the development of glucose metabolism disturbances . In addition, a safflower oil based high‐fat/high‐sucrose diet resulted in an increased abundance of Blautia . In the current study, Blautia was much higher in DM compared with NC, but in the gut of low, middle, and high dose perilla oil‐fed KKAy mice, Blautia was lower than DM.…”

Section: Discussionmentioning

confidence: 40%

Perilla Oil Supplementation Improves Hypertriglyceridemia and Gut Dysbiosis in Diabetic KKAy Mice

Wang

Zhu

et al. 2018

Molecular Nutrition Food Res

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Scope The aim of this study is to examine whether perilla oil supplementation improves glucolipid metabolism and modulates gut microbiota in diabetic KKAy mice. Methods and results The successfully established diabetic KKAy mice are randomized into four groups: diabetic model (DM), low‐dose perilla oil (LPO), middle‐dose perilla oil (MPO), and high‐dose perilla oil (HPO). C57BL/6J mice are fed a chow diet as normal control (NC). At the end of 12 weeks, mice are euthanized and glucolipid indications are analyzed. Gut microbiota analysis is carried out based on the sequencing results on V4 region of 16S rRNA. Although serum glucose, insulin, total cholesterol, low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol, abundance‐based coverage estimator, and shannon are unchanged, serum triglyceride significantly decreases in LPO compared with DM. The histopathological changes of hepatocellular macrovesicular steatosis and adipocyte hypertrophy are ameliorated by perilla oil supplementation. Blautia is significantly decreased in LPO, MPO, and HPO, compared with DM. Nonmetric multidimensional scaling analysis shows NC and LPO are relatively coherent. Conclusion These findings indicate that dietary supplementation with perilla oil can improve hypertriglyceridemia and gut dysbiosis in diabetic KKAy mice, which can be associated with potential benefits to human health.

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Section: Discussionmentioning

confidence: 40%

Perilla Oil Supplementation Improves Hypertriglyceridemia and Gut Dysbiosis in Diabetic KKAy Mice

Wang

Zhu

et al. 2018

Molecular Nutrition Food Res

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“…We also found that the relative abundance of Parasutterella in Adenine Group was significantly lower than that in Sham group, while α -ketoacid intervention tended to increase the abundance of Parasutterella . In a variety of animal models and human studies, a marked decrease of Parasutterella has been observed after high-fat diet (HFD) treatment, indicating a negative correlation was existed between the abundance of Parasutterella and HFD-induced metabolic abnormality ( Zhang et al, 2012 ; Danneskiold-Samsoe et al, 2017 ). As for Anaerovorax , it has been approved as butyrate producers ( Liu et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiome-Metabolomics Analysis Reveals the Protection Mechanism of α-Ketoacid on Adenine-Induced Chronic Kidney Disease in Rats

Huang

Zhang

et al. 2021

Front. Pharmacol.

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Objectives: As nitrogen-free precursors of corresponding essential amino, α-ketoacid have been widely prescribed to end-stage renal disease patients together with a low protein diet However, the impact of α-ketoacid on intestinal microbiota in chronic kidney disease (CKD) individuals is unknown. The study aims at investigating the variation in the intestinal microbiota and metabolic profile in response to α-ketoacid treatment in an adenine-induced CKD rat model.Design: Rats in the treatment groups were given solution of compound α-ketoacid tablets. At the end of the study, blood, feces, colon tissues and kidney tissues were collected and processed for biochemical analyses, histological and western blot analyses, 16S rRNA sequence and untargeted metabolomic analyses.Results:α-Ketoacid treatment reduced serum creatinine, blood urea nitrogen and 24 h urine protein, and alleviated tubular atrophy, glomerulosclerosis and interstitial fibrosis in adenine-induced CKD rats. Moreover, α-ketoacid significantly improved intestinal barrier and increased the abundance of Methanobrevibacter, Akkermansia, Blautia and Anaerositipes while reduced the abundance of Anaerovorax and Coprococcus_3 at the genus level. In addition, our results also demonstrated that α-ketoacid significantly reduced the concentrations of indoxyl sulfate, betaine, choline and cholesterol. Spearman’s correlation analysis revealed that the abundance of Coprococcus_3 was positively correlated with serum level of betaine, trimethylamine N-oxide, indoxyl sulfate, cholic acid and deoxycholic acid.Conclusion:α-Ketoacid has a reno-protective effect against adenine-induced CKD, which may be mediated regulation of serum metabolic profiles via affecting intestinal microbial community.

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“…The genus Parasutterella (Proteobacteria) has been defined as a core component of the healthy human and mouse gut microbiota and has been correlated with various health outcomes (33), including inflammatory bowel disease (IBD) (34), obesity (35,36), diabetes (37), fatty liver disease (38), chronic kidney disease (39), major depression (40), Henoch-Schönlein purpura in children (41), cholestasis in infants (42), and Hashimoto's thyroiditis in patients (43). A reduction of Parasutterella in response to an HFD has been observed in both animal models and human studies, indicating a positive correlation between Parasutterella abundance and HFD-induced metabolic phenotypes (44)(45)(46). Our results also showed that the abundance of Parasutterella increased significantly after the induction of the HFD, but ALA reversed this change.…”

Section: Discussionmentioning

confidence: 99%

Correlations between α-Linolenic Acid-Improved Multitissue Homeostasis and Gut Microbiota in Mice Fed a High-Fat Diet

et al. 2020

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Previous studies have shown that α-linolenic acid (ALA) has a significant regulatory effect on related disorders induced by high-fat diets (HFDs), but little is known regarding the correlation between the gut microbiota and disease-related multitissue homeostasis. We systematically investigated the effects of ALA on the body composition, glucose homeostasis, hyperlipidemia, metabolic endotoxemia and systemic inflammation, white adipose tissue (WAT) homeostasis, liver homeostasis, intestinal homeostasis, and gut microbiota of mice fed an HFD (HFD mice). We found that ALA improved HFD-induced multitissue metabolic disorders and gut microbiota disorders to various degrees. Importantly, we established a complex but clear network between the gut microbiota and host parameters. Several specific differential bacteria were significantly associated with improved host parameters. Rikenellaceae_RC9_gut_group and Parasutterella were positively correlated with HFD-induced “harmful indicators” and negatively correlated with “beneficial indicators.” Intriguingly, Bilophila showed a strong negative correlation with HFD-induced multitissue metabolic disorders and a significant positive correlation with most beneficial indicators, which is different from its previous characterization as a “potentially harmful genus.” Turicibacter might be the key beneficial bacterium for ALA-improved metabolic endotoxemia, while Blautia might play an important role in ALA-improved gut barrier integrity and anti-inflammatory effects. The results suggested that the gut microbiota, especially some specific bacteria, played an important role in the process of ALA-improved multitissue homeostasis in HFD mice, and different bacteria might have different divisions of regulation. IMPORTANCE Insufficient intake of n-3 polyunsaturated fatty acids is an important issue in modern Western-style diets. A large amount of evidence now suggests that a balanced intestinal microecology is considered an important part of health. Our results show that α-linolenic acid administration significantly improved the host metabolic phenotype and gut microbiota of mice fed a high-fat diet, and there was a correlation between the improved gut microbiota and metabolic phenotype. Some specific bacteria may play a unique regulatory role. Here, we have established correlation networks between gut microbiota and multitissue homeostasis, which may provide a new basis for further elucidating the relationship between the gut microbiota and host metabolism.

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A safflower oil based high‐fat/high‐sucrose diet modulates the gut microbiota and liver phospholipid profiles associated with early glucose intolerance in the absence of tissue inflammation

Abstract: We suggest that a diet-dependent increase in the n-6 to omega-3 (n-3) PUFA ratio in hepatic phospholipids together with gut microbiota changes contributed to early development of glucose intolerance without signs of inflammation.

Cited by 20 publications

References 47 publications

Perilla Oil Supplementation Improves Hypertriglyceridemia and Gut Dysbiosis in Diabetic KKAy Mice

Perilla Oil Supplementation Improves Hypertriglyceridemia and Gut Dysbiosis in Diabetic KKAy Mice

Microbiome-Metabolomics Analysis Reveals the Protection Mechanism of α-Ketoacid on Adenine-Induced Chronic Kidney Disease in Rats

Correlations between α-Linolenic Acid-Improved Multitissue Homeostasis and Gut Microbiota in Mice Fed a High-Fat Diet

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