A sandwich-hybridization assay for simultaneous determination of HIV and tuberculosis DNA targets based on signal amplification by quantum dots-PowerVision ™ polymer coding nanotracers

Yan, Zhongdan; Gan, Ning; Zhang, Huairong; De, Wang; Qiao, Lingling; Cao, Yuting; Li, Tianhua; Hu, Futao

doi:10.1016/j.bios.2015.04.010

Cited by 27 publications

(10 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to traditional PCR and hybridization based methods, there have been numerous reports of alternative technologies for ultrasensitive detection of nucleic acids (Lapitan et al 2015; Sun et al 2014). These have exploited innovative DNA nanotechnology schemes and a variety of readouts including electrochemical detection, quantum dots, and derivatized nanoparticles (Bernacka-Wojcik et al 2013; Hwang et al 2014; Torati et al 2016; Yan et al 2015). However, the clinical utility of these methods has not been established and few of these studies focus on drug resistance detection in Mtb.…”

Section: Discussionmentioning

confidence: 99%

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Bengtson

Homolka

Niemann

et al. 2017

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Current diagnostic tools for Mycobacterium tuberculosis (Mtb) have many disadvantages including low sensitivity, slow turnaround times, or high cost. Accurate, easy to use, and inexpensive point of care molecular diagnostic tests are urgently needed for the analysis of multidrug resistant (MDR) and extensively drug resistant (XDR) Mtb strains that emerge globally as a public health threat. In this study, we established proof-of-concept for a novel diagnostic platform (TB-DzT) for Mtb detection and the identification of drug resistant mutants using binary deoxyribozyme sensors (BiDz). TB-DzT combines a multiplex PCR with single nucleotide polymorphism (SNP) detection using highly selective BiDz sensors targeting loci associated with species typing and resistance to rifampin, isoniazid and fluoroquinolone antibiotics. Using the TB-DzT assay, we demonstrated accurate detection of Mtb and 5 mutations associated with resistance to three anti-TB drugs in clinical isolates. The assay also enables detection of a minority population of drug resistant Mtb, a clinically relevant scenario referred to as heteroresistance. Additionally, we show that TB-DzT can detect the presence of unknown mutations at target loci using combinatorial BiDz sensors. This diagnostic platform provides the foundation for the development of cost-effective, accurate and sensitive alternatives for molecular diagnostics of MDR- and XDR-TB.

show abstract

Section: Discussionmentioning

confidence: 99%

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Bengtson

Homolka

Niemann

et al. 2017

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…The next generation of techniques based on polymer complexes is the PowerVision detection system. It uses small, multifunctional polymeric connectors for the better tissue permeability than dextran and, at the same time, shows higher detection sensitivity [53].…”

Section: Enhanced Polymer One-step Staining (Epos)mentioning

confidence: 99%

“…Moreover, the method can be used in case of infectious disease such as HIV and tuberculosis for detection of DNA targets, which is possible at the level of fM (10 −15 ). Still, signal amplification techniques may also be applied, e.g., quantum dots, which yet increase the levels of detection [53].…”

Section: Enhanced Polymer One-step Staining (Epos)mentioning

confidence: 99%

Review: immunoassays in DNA damage and instability detection

Boguszewska

Szewczuk

Urbaniak

et al. 2019

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

The review includes information on the current state of knowledge of immunometric methods with emphasis on the possibility of deoxyribonucleic acid (DNA) damage detection. Beginning with basic immunoassay enzyme-linked immunosorbent assay (ELISA), this review describes methods such as tyramide signal amplification (TSA), enhanced polymer one-step staining (EPOS), and time resolved amplified cryptate emission (TRACE) as improvements of ELISA's developed over time to obtain more accurate results. In the second part of the review, surface plasmon resonance (SPR) and quantum dots (QDs) are presented as the newest outlooks in the context of immunoanalysis of biological material and molecular studies. The aim of this review is to briefly present immunoassays with emphasis on DNA damage detection; therefore, the types of methods are listed and described, types of signal indicators, basic definitions such as antigen and antibody are given. Every method is considered with an exemplary application focusing on DNA studies, DNA damage and instability detection.

show abstract

“…Magnetic gold nanoparticles (MGPs) were synthesized with our previously reported method (Yan et al, 2015). Briefly, 5 mg MGPs was transferred into 1 mL of PBS buffer that containing about 50 μL (1.0 Â 10 À 6 M) of CAP and PCB72 aptamers.…”

Section: Preparation Of the Capture Probesmentioning

confidence: 99%

A “signal-on’’ aptasensor for simultaneous detection of chloramphenicol and polychlorinated biphenyls using multi-metal ions encoded nanospherical brushes as tracers

Yan

Gan

et al. 2015

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

A sandwich-hybridization assay for simultaneous determination of HIV and tuberculosis DNA targets based on signal amplification by quantum dots-PowerVision ™ polymer coding nanotracers

Cited by 27 publications

References 33 publications

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Review: immunoassays in DNA damage and instability detection

A “signal-on’’ aptasensor for simultaneous detection of chloramphenicol and polychlorinated biphenyls using multi-metal ions encoded nanospherical brushes as tracers

Contact Info

Product

Resources

About