A scalable hyperthermic intravesical chemotherapy (HIVEC) setup for rat models of bladder cancer

Abstract

Hyperthermic intravesical chemotherapy (HIVEC)—whereby the bladder is heated to ± 43 °C during a chemotherapy instillation—can improve outcomes of non-muscle invasive bladder cancer (NMIBC) treatments. Experiments in animal models are required to explore new hyperthermia based treatments. Existing HIVEC devices are not suitable for rodents or large-scale animal trials. We present a HIVEC setup compatible with orthotopic rat models. An externally heated chemotherapeutic solution is circulated in the bladder through a double-lumen catheter with flow rates controlled using a peristaltic pump. Temperature sensors in the inflow channel, bladder and outflow channel allow temperature monitoring and adjustments in real-time. At a constant flow rate of 2.5 mL/min the system rapidly reaches the desired bladder temperature of 42–43 °C with minimal variability throughout a one-hour treatment in a rat bladder phantom, as well as in euthanised and live rats. Mean intraluminal bladder temperatures were 42.92 °C (SD = 0.15 °C), 42.45 °C (SD = 0.37 °C) and 42.52 °C (SD = 0.09 °C) in the bladder phantom, euthanised, and live rats respectively. Thermal camera measurements showed homogenous heat distributions over the bladder wall. The setup provides well-controlled thermal dose and the upscaling needed for performing large scale HIVEC experiments in rats.

Perturbation of Copper Homeostasis Sensitizes Cancer Cells to Elevated Temperature

“…Three hyperthermia-based treatments match these requirements: the already-discussed bladder CHT, hyperthermic intraperitoneal chemotherapy (HIPEC)—used for the treatment of peritoneal metastases of gastrointestinal, ovarian, and other tumors [ 70 , 71 ]—and hyperthermic isolated limb perfusion (HILP) [ 72 , 73 ]. Animal experiments that assess the efficacy of elesclomol, potentially in combination with varying copper ratios, in a bladder CHT setting [ 74 ] could pave the way for a phase I study exploring therapy-related toxicities in the short-term. Combined with more mechanistic studies, these insights will shed light on the potential of elesclomol to improve hyperthermia-based treatments in clinical cancer care.…”

Structure and magnetic properties of SiO2−FeOoskoui1, rezvani2 2023Heliyon9000View full textAdd to dashboardCite

“…But the most important problem in using these ways are the spinosity of controlling local tumor heating without damage a normal tissue [ 6 ]. In addition, in most of these treatment methods, invasive heat is used [ 7 ]. It is been reported that magnetic bioactive glass-ceramics can heat the cancer cell without damaging the normal tissue [ 8 ].…”

Cold Nanozyme for Precise Enzymatic Antitumor Immunity

“…Our recent work demonstrates the potential of external stimuli (e.g., light or ultrasound) in precise tumor therapy. 7,8 Temperature as an external stimulus has been widely applied in clinics due to its facile operation, such as hyperthermic perfusion therapy 9 or radiofrequency thermotherapy. 10 For artificial enzymes, the temperature is directly associated with their enzymatic kinetics, 11,12 thus providing a feasible means to control artificial enzymes for precise catalytic therapy.…”

“…9 NSs as a typical iron-based nanomaterial would possess multiple enzyme-like activites. The catalytic decomposition of H 2 O 2 by…”

“…9 NSs is preferably displayed in an alkalescent environment. Under temperature variation, the catalytic kinetics for POD-mimic and CATmimic activities are virtually unaffected when compared with the Bi 2 Fe 4 O 9 NS itself (FigureS10).…”