A scale-down cross-flow filtration technology for biopharmaceuticals and the associated theory

Adoptive T‐cell therapies (ATCTs) are increasingly important for the treatment of cancer, where patient immune cells are engineered to target and eradicate diseased cells. The biomanufacturing of ATCTs involves a series of time‐intensive, lab‐scale steps, including isolation, activation, genetic modification, and expansion of a patient's T‐cells prior to achieving a final product. Innovative modular technologies are needed to produce cell therapies at improved scale and enhanced efficacy. In this work, well‐defined, bioinspired soft materials are integrated within flow‐based membrane devices for improving the activation and transduction of T‐cells. Hydrogel coated membranes (HCM) functionalized with cell‐activating antibodies are produced as a tunable biomaterial for the activation of primary human T‐cells. T‐cell activation utilizing HCMs lead to highly proliferative T‐cells that express a memory phenotype. Further, transduction efficiency is improved by several folds over static conditions by using a tangential flow filtration (TFF) flow‐cell, commonly used in the production of protein therapeutics, to transduce T‐cells under flow. The combination of HCMs and TFF technology leads to increased cell activation, proliferation, and transduction compared to current industrial biomanufacturing processes. The combined power of biomaterials with scalable flow‐through transduction techniques provides future opportunities for improving the biomanufacturing of ATCTs.

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Cell Therapy Biomanufacturing: Integrating Biomaterial and Flow‐Based Membrane Technologies for Production of Engineered T‐Cells

Bomb

LeValley

Woodward

et al. 2023

Adv Materials Technologies

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Nanocomposite membrane integrated phage enrichment process for the enhancement of high rate phage infection and productivity

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Yadav

Ayyaru

et al. 2020

Biochemical Engineering Journal

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Mycobacteriophages: therapeutic approach for mycobacterial infections

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Siva Reddy,

Jain

et al. 2024

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A scale-down cross-flow filtration technology for biopharmaceuticals and the associated theory

Cited by 5 publications

References 14 publications

Cell Therapy Biomanufacturing: Integrating Biomaterial and Flow‐Based Membrane Technologies for Production of Engineered T‐Cells

Cell Therapy Biomanufacturing: Integrating Biomaterial and Flow‐Based Membrane Technologies for Production of Engineered T‐Cells

Nanocomposite membrane integrated phage enrichment process for the enhancement of high rate phage infection and productivity

Mycobacteriophages: therapeutic approach for mycobacterial infections

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