A search for novel tumour suppressor genes for adult acute leukaemia by allelotyping at sub-telomeric chromosomal regions

Giphart-Gassler, Micheline; Dalen, Arnolda De Nooij-Van; Seggelen, Vera Van Buuren-Van; Morolli, Bruno; Lohman, P.H.M.; Kluin-Nelemans, Hanneke C.

doi:10.1016/s0027-5107(98)00022-0

Cited by 4 publications

(1 citation statement)

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“…Subtelomeres are emerging as sites of active recombination and localization of specific genes (National Institute of Health and Institute of Molecular Medicine Collaboration, 1996; Van Deutekom et al , 1996; Horsley et al , 1998). In leukaemic cells, Giphart‐Gassler et al (1998) recently showed the occurrence of loss of heterozygosity in the subtelomeric regions of 4q and 21q. From present results the subtelomere of 12p emerges as the site of a recurrent breakpoint in AML with unbalanced translocations and multiple karyotypic changes.…”

Section: Discussionmentioning

confidence: 99%

Characterization of 12p molecular events outside ETV6 in complex karyotypes of acute myeloid malignancies

et al. 1999

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Summary. Acute myeloid disorders with rearrangements of 12p outside the ETV6 gene were characterized bȳ uorescence in situ hybridization (FISH) with a panel of DNA probes. Seven patients with de novo acute myeloid leukaemia (AML), one with secondary acute myeloid leukaemia (sAML), and one in the blast phase of chronic myeloid leukaemia (CML-BP) were enrolled in the study. All AML cases showed multiple karyotypic changes. Chromosome 5 and/or 7 deletions were the most frequent accompanying changes. FISH revealed ampli®cation, cryptic translocation, and fragmentation of chromosome 12, not discernible at karyotypic level. Different karyotypic rearrangements of 12p showed a common molecular event. Among the seven cases in which breakpoints could be determined, six were telomeric and one centromeric to ETV6. In three AML cases a new recurrent breakpoint in the telomeric region was identi®ed distally to locus D12S158 and to pac 922B22 which is the most telomeric probe available for 12p. Accompanying cryptic deletions were also detected in ®ve patients and the commonly deleted region, of around 700 kb, included the ETV6 gene and the D12S391 locus.

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Section: Discussionmentioning

confidence: 99%