A second-generationBacilluscell factory for rare inositol production

Abstract

Landes Bioscience aDDeNDUM S ome rare inositol stereoisomers are known to exert specific healthpromoting effects, including scylloinositol (SI), which is a promising therapeutic agent for Alzheimer disease. We recently reported a Bacillus subtilis cell factory that performed the efficient production of SI from the cheapest and most abundant isomer myo-inositol (MI). In the cell factory all "useless" genes involved in MI and SI metabolism were deleted and overexpression of the key enzymes, IolG and IolW, was appended. It converted 10 g/L MI into the same amount of SI in 48 h of cultivation. In this addendum, we discuss further improvement in the cell factory and its possible applications.

Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives

“…has been used for production of food grade amylase, glucoamylase, protease, pectinase and cellulase in varying food stuffs ( Ghani et al, 2013 ; Ouattara et al, 2017 ). Different species of Bacillus has also been used for the production of additional nutraceuticals including vitamins (e.g., riboflavin, cobalamin, inositol) and carotenoids for the synthesis of several health supplements for human consumption ( Mohammed et al, 2014 ; Tanaka et al, 2014 ; Takano, 2016 ). Nevertheless, despite above benefits, these strains have not gained much importance and attention in current functional food industry due to their relatedness with few human pathogens.…”

Two decades of new drug discovery and development for Alzheimer's disease

“…125,126 Scyllo-inositol, an endogenous inositol stereoisomer, is another antiaggregation compound, exerting specic health-promoting effects for Alzheimer disease. 127 It stabilized a small conformer of Ab42 in vitro, and neutralized cell-derived Ab oligomers in vivo. Moreover, scyllo-inositol can decrease neuronal toxicity and abate cognitive decits in multiple mouse models of AD.…”

Permeabilized Escherichia coli Whole Cells Containing Co‐Expressed Two Thermophilic Enzymes Facilitate the Synthesis of scyllo‐Inositol from myo‐Inositol

“…Compared to chemical methods, biological strategies are preferable for the synthesis of SI, even on an industrial scale, since they are environmentally benign and reaction conditions are generally mild. Bacillus subtilis cell factories were recently developed for the production of SI by modifications of inositol metabolism and culture conditions including knockout of several genes related toinositol metabolism, overexpression of key enzymes, and addition of nutrients (bacto soytone) in the medium . However, the conversion efficiency of these cell factories was limited by some factors such as metabolic factors and the efflux system of SI.…”