A Seed Treatment for Eliminating Non-hybrid Plants when using Atrazine Resistance as a Genetic Marker for Hybrid Seed Production

Conner, A. J.; Christey, M. C.

doi:10.1006/anbo.1997.0464

Cited by 6 publications

(6 citation statements)

References 7 publications

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“…Other seeds were rare selfed seed set from the male sterile parents (YA3530ms, 920S) or rare selfed seed harvested from the herbicide-sensitive pollen parents (Ilpumbyeo and Hwayeongbyeo) that were not completely eliminated by the herbicide application in the field (Table 3). This result implied that the seed harvested from these mixed population would consist of hybrid seed from the 'female' parents, which is herbicide-resistant, and non-hybrid seed resulting from self pollination of 'male' parent, which is herbicide-sensitive (Conner and Christey 1997). A common requirement for the commercial production of hybrid crops is seed purity of hybrids.…”

Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 95%

“…In fact, various innovative methods for hybrid seed production using herbicide resistance as genetic markers have been designed (Conner and Field 1995). Conner and Christey (1997) also successfully developed new approach for hybrid seed production in Brassica by using triazine-resistant, CMS lines for 'female' parents of F 1 hybrid seed, showing that non-hybrids of triazine-sensitive broccoli (Brassica oleraea L. var. italica) progeny could easily be eliminated by a seed treatment with triazine application.…”

Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 99%

“…Alternatively, we can overcome these issues by treating the F 1 seed with the herbicide to eliminate one source of contaminating seed. Conner and Christey (1997) demonstrated a simple seed treatment for the effective elimination of triazinesensitive brassica progeny.…”

Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 99%

“…If the 'female' parent has a genetic marker for herbicide resistance, the 'male' and 'female' parents could be grown as a random mixture, rather than as separate alternating blocks in the field, resulting more efficient pollen transfer, and therefore higher yield and more economical production of hybrid seed. As mentioned in the previous study (Conner and Christey 1997), the atriazine resistant, CMS Brassica plants could be grown as a random mixture with a male parent, thereby allowing The seeds harvested from the experimental plots of different treatments and the control in 2002 were grown to test hybrid seed purity. Judging from the frequency of herbicide resistance and the morphological traits of the F 1 plants, on an average, 96 and 95% of the seeds from HRPGMS mixture cultivation plots (combinations of YA3530ms/Ilpumbyeo and YA3530ms/Hwayeongbyeo) and 95% from PGMS plants in the control plot (920S/Hwayeongbyeo) were hybrid seeds.…”

Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 99%

“…The ease with which theses genetic manipulations can be made opened up a range of new applications for herbicide resistance in crop protection, agronomic practice and seed production (Conner and Field 1995). It was demonstrated that using triazine resistance as a genetic markers offered new opportunity for hybrid seed production in Brassica (Conner and Christey 1997). Currently, the transgenic or naturally mutated herbicide-resistant rice were applied for hybrid rice seed production.…”

Section: Introductionmentioning

confidence: 99%

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Use of herbicide-resistant genic male sterility in hybrid rice seed production

et al. 2007

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One of the difficult problems in hybrid rice seed production is the low outcrossing frequency and requirement for much labor to produce hybrid seeds. In order to simplify the process of hybrid rice seed production, herbicide-resistant photoperiod sensitive genic male sterile (HRPGMS) rice was utilized in this study. The herbicide resistance gene bar was transferred into the photoperiod sensitive genic male sterile (PGMS) rice 920S by Agrobacterium-mediated transformation and the HRPGMS line YA3530ms with good agronomic characteristics was bred by applying conventional pedigree breeding technique. The seeds of HRPGMS and pollen parent were mixed with the ratio of 4:1 in weight, and were sowed in seedling box. The mixed seedlings of HRPGMS and pollen parent grown for 30 days were transplanted by the small transplanting machine in the field. The herbicide glufosinate ammonium was sprayed at 7 days after flowering to kill all the plants of pollen parent, whereas hybrid seeds were harvested from the survived HRPGMS parent at maturity. The outcrossing frequency of HRPGMS line from two combinations in 2002 and from five combinations in 2004 were compared with a control cultivated by the conventional 2-line system. As the result, the mean outcrossing frequency in HRPGMS of the treatments were 10.6-24.5% compared with 5.5% in PGMS of the control in 2002, and that were 24.7-32.0% compared with 7.5% in the control in 2004. Consequently, using HRPGMS in two-line system was proved to be a new method that would simplify the process of hybrid rice seed production and to increase outcrossing frequency without any artificial supplementary pollination processes.

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Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 95%

Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 99%

Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 99%

Section: Elimination Of Pollen Parent By Herbicide Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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