A selective and differential medium for Vibrio harveyi

Harris, Lachlan; Owens, Leigh; Smith, Shawn

doi:10.1128/aem.62.9.3548-3550.1996

Cited by 51 publications

(28 citation statements)

References 13 publications

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“…After 75 days of feeding trial, shrimps were challenged with virulent strain of Vibrio harveyi isolated on TCBS agar from disease affected shrimp pond. It was further characterized on Vibrio harveyi agar and by biochemical tests (Harris, Owens & Smith ). Bacterium was inoculated in tryptic soya broth (1% w/v NaCl) for 18 h at 28°C.…”

Section: Methodsmentioning

confidence: 99%

Effects of biofloc under different carbon sources and protein levels on water quality, growth performance and immune responses in black tiger shrimpPenaeus monodon(Fabricius, 1978)

Kumar

Anand

et al. 2015

Aquac Res

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A 75-day 2 9 3 factorial experiment was conducted to evaluate the effect of two levels of dietary protein (32 and 40%) and two different carbon sources (rice flour-R and molasses-M), and without carbohydrate (control-C) in black tiger shrimp Penaeus monodon juveniles (3.37 AE 0.04 g) at 100 nos. m À3 in 100 L fibre reinforced plastic tanks. Biofloc volume and total suspended solid were higher in molasses added groups (32 + M and 40 + M) followed by rice flour (32 + R and 40 + R) and controls (32C and 40C). Molasses and rice flour addition significantly reduced (P < 0.01) the total ammonia-N compared to controls. The highest Vibrio, Bacillus and Lactobacillus counts were recorded in 40 + M, 32 + M and 32 + R respectively. Among the treatments, significantly higher (P < 0.01) final body weight was recorded in 40 + R (8.5 AE 0.3 g), 40 + M (7.8 AE 0.3 g) and 32 + R (7.5 AE 0.4 g) compared to control groups, 32C (6.1 AE 0.3 g), 40C (6.4 AE 0.3 g) and molasses added group, 32 + M (5.7 AE 0.4 g). Rice flour supplementation significantly increased (P < 0.01) the total haemocyte count (910 6 cells mL À1 ) in 32 + R (45.7 AE 3.7) and 40 + R (44.3 AE 3.1) compared to controls, 32C (27.3 AE 3.4) and 40C (25.8 AE 0.9). Similarly, higher superoxide dismutase, catalase, serum protein and glucose were recorded in the rice flour added groups, 40 + R followed by 32 + R. Among the treatments, the highest level of prophenoloxidase (OD 490 nm, 0.3 AE 0.0) and survival after challenge with Vibrio harveyi (55.6%) was observed in 32 + R. The study elucidates that rice flour addition produces optimum level of biofloc with better growth and immune responses compared to molasses and control. Furthermore, rice flour addition at 32% protein level could replace 40% protein feed.

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Section: Methodsmentioning

confidence: 99%

Effects of biofloc under different carbon sources and protein levels on water quality, growth performance and immune responses in black tiger shrimpPenaeus monodon(Fabricius, 1978)

Kumar

Anand

et al. 2015

Aquac Res

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“…Shrimp larvae affected with luminescent bacterial disease, commercial shrimp hatchery water samples, seabass fingerlings, lobster and rotifers were processed for occurrence of V. harveyi using tryptose soy agar (TSA) fortified with 1% sodium chloride and 0AE3% glycerol and a selective medium (Harris et al 1996). Characteristic colonies on Harris' medium (small light green colonies with dark green centres and yellow halo) and luminescent colonies on TSA were phenotypically characterized according to the protocols of Smibert and Krieg (1991) and identified (Bauman and Schubert 1984;Alsina and Blanch 1994).…”

Section: Harveyi Isolatesmentioning

confidence: 99%

Phenotypic and molecular typing ofVibrio harveyiisolates and their pathogenicity to tiger shrimp larvae

Alavandi

Manoranjita

Vijayan

et al. 2006

Letters in Applied Microbiology

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Aims: The objective of the present study was to identify the biotype(s) and molecular type(s) of Vibrio harveyi associated with pathogenicity in tiger shrimp (Penaeus monodon) larvae. Methods and Results: Five luminescent and four nonluminescent V. harveyi isolates were subjected to phenotyping and random amplified polymorphic DNA (RAPD) fingerprinting, and pathogenicity testing to P. monodon mysis. Four isolates induced 34–41% mortality of P. monodon mysis when challenged at the rate of 106 CFU ml−1 within 60 h. Sucrose‐fermenting biotypes of V. harveyi appeared to be associated with pathogenicity to larval shrimp. Higher temperature and salinity appeared to play a role on the onset of vibriosis and mortality in the challenged larval shrimp. Pathogenic isolates of V. harveyi could be demarcated as revealed by their clustering in the dendrogram constructed based on the RAPD fingerprints. Conclusions: Nonluminescent V. harveyi also appear to be important aetiological agents of vibriosis of shrimp larvae. Sucrose‐fermenting biotypes are likely to be pathogenic. High temperature may trigger onset of vibriosis. Significance and Impact of the Study: Biotyping of V. harveyi isolates and looking for traits, such as ability to ferment sucrose may be helpful in identifying the pathogenic forms, and such approach requires to be investigated further with larger number of isolates.

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“…Normal aerobic metabolism generates large quantities of reactive oxygen species (ROS) including superoxide anions (O 3 2 ), peroxide (O 23 2 ) and hydroxyl radicals ( OH) [4]. Furthermore, ROS are an important component of the active defense response mediated against in-vading microorganisms by crustacean hemocytes [5].…”

Section: Introductionmentioning

confidence: 99%

Unusual adaptive, cross protection responses and growth phase resistance against peroxide killing in a bacterial shrimp pathogen,Vibrio harveyi

Vattanaviboon¹,

Mongkolsuk²

2001

FEMS Microbiology Letters

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Oxidant induced protection against peroxide killing was investigated in a prawn bacterial pathogen, Vibrio harveyi. Exposure to 250 microM H(2)O(2) induced adaptive protection against subsequent exposure to killing concentrations of H(2)O(2). In addition, 200 microM t-butyl hydroperoxide (tBOOH) induced cross protection to H(2)O(2) killing. On the other hand, peroxide pretreatment did not induce protection against tBOOH killing. Peroxide induced adaptive and cross protection responses required new protein synthesis and were abolished by addition of a protein synthesis inhibitor. Pretreatments of V. harveyi with 250 microM H(2)O(2) and 200 microM tBOOH induced an increase in peroxide scavenging enzymes, catalase and alkyl hydroperoxide reductase subunit C. In addition, stationary phase cells of V. harveyi were more resistant to H(2)O(2) and iodoacetamide killing but highly susceptible to tBOOH killing compared to exponential phase cells. Many aspects of the oxidative stress response of V. harveyi are different from those of other bacteria and these factors may be important for bacterial survival in the environment and during interactions with host shrimp.

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A selective and differential medium for Vibrio harveyi

Cited by 51 publications

References 13 publications

Effects of biofloc under different carbon sources and protein levels on water quality, growth performance and immune responses in black tiger shrimpPenaeus monodon(Fabricius, 1978)

Effects of biofloc under different carbon sources and protein levels on water quality, growth performance and immune responses in black tiger shrimpPenaeus monodon(Fabricius, 1978)

Phenotypic and molecular typing ofVibrio harveyiisolates and their pathogenicity to tiger shrimp larvae

Unusual adaptive, cross protection responses and growth phase resistance against peroxide killing in a bacterial shrimp pathogen,Vibrio harveyi

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