A Selective Medium for the Isolation of Vibrio parahaemolyticus II. BTB-TTGA Agar

Fujiwara, Kikuo; Tsuchiya, Yasuko; Kikuchi, Terukichi; Mori, Yoshiji

doi:10.3358/shokueishi.6.437

J. Food Hyg. Soc. Jpn

1965

DOI: 10.3358/shokueishi.6.437

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A Selective Medium for the Isolation of Vibrio parahaemolyticus II. BTB-TTGA Agar

Kikuo Fujiwara

Yasuko Tsuchiya

Terukichi Kikuchi

et al.

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1971

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“…The growth of competitive organisms, however, made isolation and identification of V. parahaemolyticus from sea fish and sea fish products difficult. Consequently, new selective media were proposed and used by Japanese investigators (2,(6)(7)(8)10). It is not apparent that these new media resulted from systematic studies of the effect of each of their constitutents on the growth of V. parahaemolyticus or competitive species typical of the marine environment.…”

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Modified Selective and Differential Isolation Medium for Vibrio parahaemolyticus

Twedt

Novelli

1971

Applied Microbiology

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The semiselective salt-starch-agar formulation of Baross and Liston was modified as the result of a systematic study of the effect of each constituent on the growth of Vibrio parahaemolyticus and competitive species characteristic of the marine environment. The selection of nutrient constituents depended on an analysis of their effect on generation time. The addition of inhibitors depended on an analysis of minimal inhibitory concentrations. The modified formulation included: peptone, 2.0%; yeast extract, 0.2%; corn starch, 0.5%; NaCl, 3.0%; agar, 1.5%c (pH 8.0). Penicillin at 2 to 5 units/ml increased selectivity without significantly inhibiting Vibrio in pure suspensions. Over 62% of the most sensitive strain (YM-K33) was recovered at a concentration of 5 units of penicillin per ml. The per cent recovery of V. parahaemolyticus from fish homogenate compared favorably with other selective formulations. At an initial concentration of 105 cells/ml, recovery varied with the strain used from 60 to 119%, whereas at 102 cells/ml the range was 36 to 94%. Applications of the medium for Vibrio quantification are discussed.

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Modified Selective and Differential Isolation Medium for Vibrio parahaemolyticus

Twedt

Novelli

1971

Applied Microbiology

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A Selective Medium for the Isolation of Vibrio parahaemolyticus II. BTB-TTGA Agar

Cited by 1 publication

References 0 publications

Modified Selective and Differential Isolation Medium for Vibrio parahaemolyticus

Modified Selective and Differential Isolation Medium for Vibrio parahaemolyticus

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