A SELECTIVE MEDIUM FOR THE ISOLATION OF VIBRIO FETUS AND RELATED VIBRIOS

Abstract

report there were some which needed adjustment of pH to the optimal value (7.2) with sodium bicarbonate after being added into the basal medium. These were L-glutamic acid, DL-aspartic acid, L-arginine hydrochloride, DL-lysine dihydrochloride, L-histidine hydrochloride and DL-ornithine hydrochloride. To correct the errors the experiments have been done using sodium hydroxide as a neutralizing substance in place of sodium bicarbonate. The results are shown in table 3. In each case the rate of the germination in the media containing caramel from glucose is higher than that reported in the previous report except in the case of L-glutamic acid. Hence conclusions mentioned in the previous report have partially to be corrected. DL-Aspartic acid, L-arginine and DL-ornithine may belong to the group which has a stimulative effect on both the growth and the germination.