A self-indicating cellulose-based gel with tunable performance for bioactive agent delivery

Lai, Wing‐Fu; Gui, Dayong; Wong, Mangin; Döring, Aaron; Rogach, Andrey L.; He, Tiancheng; Wong, Wing Tak

doi:10.1016/j.jddst.2021.102428

Cited by 47 publications

(23 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These cells are selected because their viable rates are substratedependent, making them extensively used in the literature to evaluate the cytotoxicity of an agent of interest. 50,51 After treatment with the nanocomposites for 24 h at a concentration as high as 100 µg/mL, over 80% of the cells are still viable. This suggests that the toxicity of nanocomposites varies with the cell type, being highly toxic to MCF-7 cells but showing good biocompatibility with 3T3 fibroblasts.…”

Section: Cytotoxic Effects and Ros-generating Capacitymentioning

confidence: 99%

ROS-Generating Amine-Functionalized Magnetic Nanoparticles Coupled with Carboxymethyl Chitosan for pH-Responsive Release of Doxorubicin

Obireddy¹,

Lai²

2022

IJN

Self Cite

View full text Add to dashboard Cite

Magnetic nanoparticles have been used in diverse pharmaceutical applications because they can potentially be used to target specific sites. In the present work, a new type of nanocomposites is designed as a carrier of controlled bioactive agent delivery. Methods: Amine-functionalized magnetic nanoparticles (amine-MNPs) are coupled with carboxymethyl chitosan (CMC) to generate the nanocomposites, namely MNPs-CMC, which can be further loaded with doxorubicin (DOX) to produce MNPs-CMC-DOX. The generated nanocomposites are characterized by using various techniques (including FTIR, 1 H-NMR, DSC, TGA, SEM, TEM and XRD). In vitro drug release studies are conducted in PBS with different pH values (1.2 and 6.8) at different temperatures (25°C and 37°C). The toxicity of the nanocomposites is tested in MCF-7 and 3T3 cells. The ROS-generating capacity of the nanocomposites is determined in treated cells using 2',7'-dichlorodihydrofluorescein diacetate. Results: The structures of MNPs, CMC, and nanocomposites are confirmed by FTIR, XRD, and 1 H-NMR data reveals the formation of CMC from chitosan (CS). The size of MNPs is estimated by TEM to be around 25 nm. After conjugation with CMC, the size of the nanocomposites increases to 46-57 nm. Based on the release profiles of MNPs-CMC-DOX, our nanocomposites are pH-responsive. In addition, our nanocomposites show reactive oxygen species (ROS)-generating capacity and cell type-dependent toxicity. Conclusion: Our nanocomposites show high potential for use in bioactive agent delivery. Along with their ROS-generating capacity, they warrant further development as pH-responsive carriers for therapeutic applications.

show abstract

Section: Cytotoxic Effects and Ros-generating Capacitymentioning

confidence: 99%

ROS-Generating Amine-Functionalized Magnetic Nanoparticles Coupled with Carboxymethyl Chitosan for pH-Responsive Release of Doxorubicin

Obireddy¹,

Lai²

2022

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…In tissue engineering and regenerative medicine, they have been used as an excellent option for developing and creating innovative medical textile and nanoarchitecture tissue scaffolds and grafts [ 83 ]. Studies have shown that plant polymers, particularly carbohydrates, made into gel systems, serve as drug carriers for a variety of medicinal applications [ 84 , 85 , 86 , 87 , 88 ]. Besides their use as drug carriers, these carbohydrate polymers serve also as reporters that monitor drug delivery performance [ 84 ].…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that plant polymers, particularly carbohydrates, made into gel systems, serve as drug carriers for a variety of medicinal applications [ 84 , 85 , 86 , 87 , 88 ]. Besides their use as drug carriers, these carbohydrate polymers serve also as reporters that monitor drug delivery performance [ 84 ]. Other plant-based materials such as chiral protein superparticles now show promises in immunotherapy and tumor suppression [ 89 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Virulence Activity of 3,3′-Diindolylmethane (DIM): A Bioactive Cruciferous Phytochemical with Accelerated Wound Healing Benefits

et al. 2022

View full text Add to dashboard Cite

Antimicrobial resistance is among the top global health problems with antibacterial resistance currently representing the major threat both in terms of occurrence and complexity. One reason current treatments of bacterial diseases are ineffective is the occurrence of protective and resistant biofilm structures. Phytochemicals are currently being reviewed for newer anti-virulence agents. In the present study, we aimed to investigate the anti-virulence activity of 3,3′-diindolylmethane (DIM), a bioactive cruciferous phytochemical. Using a series of in vitro assays on major Gram-negative pathogens, including transcriptomic analysis, and in vivo porcine wound studies as well as in silico experiments, we show that DIM has anti-biofilm activity. Following DIM treatment, our findings show that biofilm formation of two of the most prioritized bacterial pathogens Acinetobacter baumannii and Pseudomonas aeruginosa was inhibited respectively by 65% and 70%. Combining the antibiotic tobramycin with DIM enabled a high inhibition (94%) of P. aeruginosa biofilm. A DIM-based formulation, evaluated for its wound-healing efficacy on P. aeruginosa-infected wounds, showed a reduction in its bacterial bioburden, and wound size. RNA-seq was used to evaluate the molecular mechanism underlying the bacterial response to DIM. The gene expression profile encompassed shifts in virulence and biofilm-associated genes. A network regulation analysis showed the downregulation of 14 virulence-associated super-regulators. Quantitative real-time PCR verified and supported the transcriptomic results. Molecular docking and interaction profiling indicate that DIM can be accommodated in the autoinducer- or DNA-binding pockets of the virulence regulators making multiple non-covalent interactions with the key residues that are involved in ligand binding. DIM treatment prevented biofilm formation and destroyed existing biofilm without affecting microbial death rates. This study provides evidence for bacterial virulence attenuation by DIM.

show abstract

“…Nanotechnology development has provided a wide range of nanomaterial designs that are useful and necessary in modern people’s lives, with applications in both technical and biomedical fields. In the sanitary domain, gelatin-based nanofiber dressings were found to be very useful for wound healing applications [ 1 ]; in biomedicine, therapeutic nanoparticles loaded with pharmaceutical agents have been used, such as, for example, gel beads and fibers for co-delivery of multiple agents, [ 2 ] or super molecule nanoparticles for tumor suppression and synergistic immunotherapy [ 3 ], and exosome-nanoparticles for treating brain disorders [ 4 ]. However, our knowledge about the interactions of nanomaterials with living cells hasn’t kept up with this development and is still not understood well enough.…”

Section: Introductionmentioning

confidence: 99%

Aspartic Acid Stabilized Iron Oxide Nanoparticles for Biomedical Applications

et al. 2022

View full text Add to dashboard Cite

Aspartic acid stabilized iron oxide nanoparticles (A-IONPs) with globular shape and narrow size distribution were prepared by the co-precipitation method in aqueous medium. A quantum-mechanical approach to aspartic acid optimized structure displayed negative charged sites, relatively high dipole moment, and hydrophilicity, which recommended it for interaction with iron cations and surrounding water electrical dipoles. A-IONPs were characterized by TEM, XRD, ATR-FTIR, EDS, DSC, TG, DLS, NTA, and VSM techniques. Theoretical study carried out by applying Hartree-Fock and density functional algorithms suggested that some aspartic acid properties related to the interaction can develop with nanoparticles and water molecules. The results of experimental investigation showed that the mean value of particle physical diameters was 9.17 ± 2.2 nm according to TEM image analysis, the crystallite size was about 8.9 nm according to XRD data, while the magnetic diameter was about 8.8 nm, as was determined from VSM data interpretation with Langevin’s theory. The A-IONP suspension was characterized by zeta-potential of about −11.7 mV, while the NTA investigation revealed a hydrodynamic diameter of 153.9 nm. These results recommend the A-IONP suspension for biomedical applications.

show abstract

A self-indicating cellulose-based gel with tunable performance for bioactive agent delivery

Cited by 47 publications

References 37 publications

ROS-Generating Amine-Functionalized Magnetic Nanoparticles Coupled with Carboxymethyl Chitosan for pH-Responsive Release of Doxorubicin

ROS-Generating Amine-Functionalized Magnetic Nanoparticles Coupled with Carboxymethyl Chitosan for pH-Responsive Release of Doxorubicin

Anti-Virulence Activity of 3,3′-Diindolylmethane (DIM): A Bioactive Cruciferous Phytochemical with Accelerated Wound Healing Benefits

Aspartic Acid Stabilized Iron Oxide Nanoparticles for Biomedical Applications

Contact Info

Product

Resources

About